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Effects of modulators of phosphoryla...

O'Beirne, Carole P.

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Effects of modulators of phosphorylation on P-glycoprotein in Multi-Drug Resistant Chinese Hamster Ovary cells.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Effects of modulators of phosphorylation on P-glycoprotein in Multi-Drug Resistant Chinese Hamster Ovary cells./
Author:	O'Beirne, Carole P.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 1992,
Description:	98 p.
Notes:	Source: Masters Abstracts International, Volume: 31-03, page: 1223.
Contained By:	Masters Abstracts International31-03.
Subject:	Pharmacology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MM73981
ISBN:	9780315739819

Effects of modulators of phosphorylation on P-glycoprotein in Multi-Drug Resistant Chinese Hamster Ovary cells.
O'Beirne, Carole P.

Effects of modulators of phosphorylation on P-glycoprotein in Multi-Drug Resistant Chinese Hamster Ovary cells. - Ann Arbor : ProQuest Dissertations & Theses, 1992 - 98 p.

Source: Masters Abstracts International, Volume: 31-03, page: 1223.

Thesis (M.Sc.)--University of Toronto (Canada), 1992.

It was the objective of this study to determine the pattern of phosphorylation of P-glycoprotein in Multi-Drug Resistant (MDR) Chinese Hamster Ovary (CHO) cells selected for colchicine resistance upon modulation with TPA, a protein kinase C inhibitor, staurosporine, a protein kinase inhibitor and okadaic acid, a phosphatase 1 and 2A inhibitor and to investigate whether such modulation, if present, effects IC50 values and drug transport profiles in these MDR cells. The use of TPA, staurosporine and okadaic acid in intact cell phosphorylation experiments failed to result in any significant changes in the level of phosphorylation of P-glycoprotein when compared to the control level. Results suggest that protein kinase C activity is unrelated to MDR in these cells. TPA and okadaic acid did not elicit changes in the MDR cells' response to the cytotoxic drug daunomycin and similarly failed to affect the efflux pump action of P-glycoprotein, in accumulation experiments. Staurosporine's effects on these parameters, however, were markedly different. Results of phosphorylation experiments with the calcium channel blocker verapamil suggest that this compound's sensitizing effect on MDR CHO cells is not the result of phosphorylation modulation. (Abstract shortened by UMI.).

ISBN: 9780315739819Subjects--Topical Terms:

634543
Pharmacology.

Effects of modulators of phosphorylation on P-glycoprotein in Multi-Drug Resistant Chinese Hamster Ovary cells.
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100 1 $a O'Beirne, Carole P. $3 3285331
245 1 0 $a Effects of modulators of phosphorylation on P-glycoprotein in Multi-Drug Resistant Chinese Hamster Ovary cells.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 1992
300 $a 98 p.
500 $a Source: Masters Abstracts International, Volume: 31-03, page: 1223.
502 $a Thesis (M.Sc.)--University of Toronto (Canada), 1992.
520 $a It was the objective of this study to determine the pattern of phosphorylation of P-glycoprotein in Multi-Drug Resistant (MDR) Chinese Hamster Ovary (CHO) cells selected for colchicine resistance upon modulation with TPA, a protein kinase C inhibitor, staurosporine, a protein kinase inhibitor and okadaic acid, a phosphatase 1 and 2A inhibitor and to investigate whether such modulation, if present, effects IC50 values and drug transport profiles in these MDR cells. The use of TPA, staurosporine and okadaic acid in intact cell phosphorylation experiments failed to result in any significant changes in the level of phosphorylation of P-glycoprotein when compared to the control level. Results suggest that protein kinase C activity is unrelated to MDR in these cells. TPA and okadaic acid did not elicit changes in the MDR cells' response to the cytotoxic drug daunomycin and similarly failed to affect the efflux pump action of P-glycoprotein, in accumulation experiments. Staurosporine's effects on these parameters, however, were markedly different. Results of phosphorylation experiments with the calcium channel blocker verapamil suggest that this compound's sensitizing effect on MDR CHO cells is not the result of phosphorylation modulation. (Abstract shortened by UMI.).
590 $a School code: 0779.
650 4 $a Pharmacology. $3 634543
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