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Design and Development of Electroche...

Liu, Xiaobo.

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Design and Development of Electrochemical Biosensors for Real-time Monitoring of Oxidative Stress and Efficient Bacteria Detection.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Design and Development of Electrochemical Biosensors for Real-time Monitoring of Oxidative Stress and Efficient Bacteria Detection./
Author:	Liu, Xiaobo.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
Description:	159 p.
Notes:	Source: Dissertation Abstracts International, Volume: 78-09(E), Section: B.
Contained By:	Dissertation Abstracts International78-09B(E).
Subject:	Analytical chemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10269659
ISBN:	9781369748123

Design and Development of Electrochemical Biosensors for Real-time Monitoring of Oxidative Stress and Efficient Bacteria Detection.
Liu, Xiaobo.

Design and Development of Electrochemical Biosensors for Real-time Monitoring of Oxidative Stress and Efficient Bacteria Detection. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 159 p.

Source: Dissertation Abstracts International, Volume: 78-09(E), Section: B.

Thesis (Ph.D.)--Clarkson University, 2017.

The need for simple, rapid, cost-effective and field-portable screening methods has boosted the development of biosensors in various fields, ranging from medical diagnosis to food safety and environmental monitoring applications. The current analytical methodologies rely on the use of laboratory-based instrumentation which are complex, expensive and require skilled personnel. In addition to the broad range of applications, biosensors can be used to study biologically-relevant molecules, their quantitation, kinetics and release in biological models. This thesis describes design and development of electrochemical biosensors for the detection of reactive oxygen and nitrogen species (ROS/RNS) that play a major role in oxidative stress. Performance characteristics and applicability of these biosensors to the study of oxidative stress-related mechanisms in bacterial cells, zebrafish embryos, and in a mouse-model of the mouse model of intestinal ischemia-reperfusion injury is demonstrated. A label-free electrochemical biosensor that utilizes the recognition properties of synthetic antimicrobial peptides for bacteria is also described.

ISBN: 9781369748123Subjects--Topical Terms:

3168300
Analytical chemistry.

Design and Development of Electrochemical Biosensors for Real-time Monitoring of Oxidative Stress and Efficient Bacteria Detection.
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245 1 0 $a Design and Development of Electrochemical Biosensors for Real-time Monitoring of Oxidative Stress and Efficient Bacteria Detection.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2017
300 $a 159 p.
500 $a Source: Dissertation Abstracts International, Volume: 78-09(E), Section: B.
500 $a Adviser: Silvana Andreescu.
502 $a Thesis (Ph.D.)--Clarkson University, 2017.
520 $a The need for simple, rapid, cost-effective and field-portable screening methods has boosted the development of biosensors in various fields, ranging from medical diagnosis to food safety and environmental monitoring applications. The current analytical methodologies rely on the use of laboratory-based instrumentation which are complex, expensive and require skilled personnel. In addition to the broad range of applications, biosensors can be used to study biologically-relevant molecules, their quantitation, kinetics and release in biological models. This thesis describes design and development of electrochemical biosensors for the detection of reactive oxygen and nitrogen species (ROS/RNS) that play a major role in oxidative stress. Performance characteristics and applicability of these biosensors to the study of oxidative stress-related mechanisms in bacterial cells, zebrafish embryos, and in a mouse-model of the mouse model of intestinal ischemia-reperfusion injury is demonstrated. A label-free electrochemical biosensor that utilizes the recognition properties of synthetic antimicrobial peptides for bacteria is also described.
520 $a The first section of the thesis discusses the development and characterization of a cytochrome c --based superoxide biosensor to study the mechanism of antibiotics-meditated oxidative stress in bacteria. This work provides a quantitative methodology and fundamental knowledge to further explore the role of oxidative stress in antibiotics-meditated bacterial death and assessment of physiological changes associated with the complex metabolic events related to oxidative stress and bacterial resistance. The second sectiondescribes miniaturization and application of the cytochrome c biosensor for real time in vivo monitoring of superoxide radicals in a mice intestine model of ischemia and reperfusion. The third part describes design and development of an implantable electrochemical sensor for measuring nitric oxide (NO) release in embryonic zebrafish. This sensor was further used to establish the involvement of nitrosative stress in the mechanisms of nanosilver toxicity in a zebrafish model. Lastly, a label free electrochemical biosensor was developed for the rapid and sensitive detection of bacterial pathogens. This part introduces the use of modularly designed and site-specifically oriented synthetic antimicrobial peptides (sAMP) as novel recognition agents for detection of bacterial pathogens. The approach also enabled differentiation between live and dead bacteria. The proposed methodology provides a universal platform for the detection of bacterial pathogens based on engineered peptides, as alternative to the most commonly used immunological and gene based assays. The method can also be used to fabricate antimicrobial coatings and surfaces for inactivation and screening of viable bacteria.
520 $a The outcome of the thesis is the development of electrochemical biosensors for rapid real-time detection of ROS/RNS species in biological settings, and for monitoring pathogenic bacteria. The availability of sensitive, specific and robust biosensors could enable future developments of portable technology for field monitoring and methods that could facilitate fundamental research to answer mechanistic questions in complex biological systems.
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650 4 $a Biochemistry. $3 518028
650 4 $a Microbiology. $3 536250
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710 2 $a Clarkson University. $b Chemistry. $3 3177158
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