東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

The ecology of Vibrio vulnificus and...

Drake, Stephenie Lynn.

FindBook

Google Book

Amazon

博客來

The ecology of Vibrio vulnificus and Vibrio parahaemolyticus from oyster harvest sites in the Gulf of Mexico.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The ecology of Vibrio vulnificus and Vibrio parahaemolyticus from oyster harvest sites in the Gulf of Mexico./
作者:	Drake, Stephenie Lynn.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2008,
面頁冊數:	262 p.
附註:	Source: Dissertation Abstracts International, Volume: 70-01, Section: B, page: 1350.
Contained By:	Dissertation Abstracts International70-01B.
標題:	Biological oceanography. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3345358
ISBN:	9781109005493

The ecology of Vibrio vulnificus and Vibrio parahaemolyticus from oyster harvest sites in the Gulf of Mexico.
Drake, Stephenie Lynn.

The ecology of Vibrio vulnificus and Vibrio parahaemolyticus from oyster harvest sites in the Gulf of Mexico. - Ann Arbor : ProQuest Dissertations & Theses, 2008 - 262 p.

Source: Dissertation Abstracts International, Volume: 70-01, Section: B, page: 1350.

Thesis (Ph.D.)--North Carolina State University, 2008.

The Vibrionaceae are environmentally ubiquitous to estuarine waters. Two species in particular, V. vulnificus and V. parahaemolyticus, are important human pathogens that are transmitted by the consumption of contaminated molluscan shellfish. There is limited information available for the recent risk assessments; accordingly, the purpose of this study was to address some of these data gaps in the V. vulnificus and V. parahaemolyticus risk assessments. The objectives of this study were to (i) quantify the levels of total estuarine bacteria, total Vibrio spp., and specific levels of non-pathogenic and pathogenic V. vulnificus and V. parahaemolyticus over the harvest period; and (ii) determine if length of harvest time affects the levels of V. vulnificus.

ISBN: 9781109005493Subjects--Topical Terms:

2122748
Biological oceanography.

The ecology of Vibrio vulnificus and Vibrio parahaemolyticus from oyster harvest sites in the Gulf of Mexico.
LDR:04615nmm a2200361 4500 001 2116884
005 20170508094349.5
008 180830s2008 ||||||||||||||||| ||eng d
020 $a 9781109005493
035 $a (MiAaPQ)AAI3345358
035 $a AAI3345358
040 $a MiAaPQ $c MiAaPQ
100 1 $a Drake, Stephenie Lynn. $3 3278632
245 1 4 $a The ecology of Vibrio vulnificus and Vibrio parahaemolyticus from oyster harvest sites in the Gulf of Mexico.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2008
300 $a 262 p.
500 $a Source: Dissertation Abstracts International, Volume: 70-01, Section: B, page: 1350.
500 $a Adviser: Lee-Ann Jaykus.
502 $a Thesis (Ph.D.)--North Carolina State University, 2008.
520 $a The Vibrionaceae are environmentally ubiquitous to estuarine waters. Two species in particular, V. vulnificus and V. parahaemolyticus, are important human pathogens that are transmitted by the consumption of contaminated molluscan shellfish. There is limited information available for the recent risk assessments; accordingly, the purpose of this study was to address some of these data gaps in the V. vulnificus and V. parahaemolyticus risk assessments. The objectives of this study were to (i) quantify the levels of total estuarine bacteria, total Vibrio spp., and specific levels of non-pathogenic and pathogenic V. vulnificus and V. parahaemolyticus over the harvest period; and (ii) determine if length of harvest time affects the levels of V. vulnificus.
520 $a Oyster and water samples were harvested seasonally from 3 U.S. Gulf Coast sites over 2 years. Environmental parameters were monitored during harvesting. Both surface and bottom water samples (1 L) were taken at the beginning of harvesting and at the end of harvesting. Oyster samples (15 specimens for each time point) were taken at 0, 2.5, 5.0, 7.5, and 10 hrs intervals after being held at ambient temperature during harvesting. Samples were processed for many different bacteria. For enumeration of total V. parahaemolyticus , pathogenic V. parahaemolyticus, and V. vulnificus was done using colony lift hybridization (tlh, tdh+ and/or trh+, and vvhA gene targets, respectively).
520 $a MPN methods were also used to obtain estimates of pathogenic V. parahaemolyticus (tdh+ and/or trh+) counts. Representative V. parahaemolyticus and V. vulnificus isolates were subjected to phenotyping; V. vulnificus isolates were also subjected to genotyping. Different statistical analysis were used to establish relationships where appropriate.
520 $a The first manuscript describes the field study which examined the distribution and variation in the levels of V. parahaemolyticus and V. vulnificus (both total and pathogenic strains) in shellfish and their overlay waters, and established the effect of seasonal and environmental/ecological factors on these distributions. The best estimate of growth for V. parahaemolyticus (tlh) in oysters based on water temperature was 0.054 log10 per °C, while the best estimate for growth for V. vulnificus (vvhA) in oysters based on water temperature was 0.068 log10 per °C. Estimated relationships between tlh and vvhA growth rates and air temperature were consistent with the risk assessments. No statistical relationship could be established between pathogenic V. parahaemolyticus ( tdh+ and/or trh+) growth rates and air temperatures.
520 $a In the second study, we specifically looked at the effect of extended boat deck storage of commercially harvested oysters held at ambient air temperature on the levels of V. vulnificus. During summer, increases in V. vulnificus were as high as 1.4 log10 (CFU/g) after 10 h storage at ambient air temperatures; for spring/fall oysters, a 1.0 log10 increase was observed; and in winter, increases were <0.5 log10. Statistically significant (p<0.05) correlations between ambient air temperature and increases in V. vulnificus counts were noted.
520 $a Taken together, this research provides information which can be used to fill key data gaps in the current risk assessments for V. vulnificus and V. parahaemolyticus. These data can be used in future iterations of the risk assessments to help better predict risk and inform policy as risk managers seek to reduce the disease burden associated with these important foodborne pathogens.
590 $a School code: 0155.
650 4 $a Biological oceanography. $3 2122748
650 4 $a Microbiology. $3 536250
650 4 $a Public health. $3 534748
690 $a 0416
690 $a 0410
690 $a 0573
710 2 $a North Carolina State University. $3 1018772
773 0 $t Dissertation Abstracts International $g 70-01B.
790 $a 0155
791 $a Ph.D.
792 $a 2008
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3345358