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Living in a digital world: features and applications of FPGA in photon detection.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Living in a digital world: features and applications of FPGA in photon detection./
作者:	Arnesano, Cosimo.
面頁冊數:	103 p.
附註:	Source: Dissertation Abstracts International, Volume: 74-09(E), Section: B.
Contained By:	Dissertation Abstracts International74-09B(E).
標題:	Biomedical engineering. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3564565
ISBN:	9781303135989

Living in a digital world: features and applications of FPGA in photon detection.
Arnesano, Cosimo.

Living in a digital world: features and applications of FPGA in photon detection. - 103 p.

Source: Dissertation Abstracts International, Volume: 74-09(E), Section: B.

Thesis (Ph.D.)--University of California, Irvine, 2013.

Optical spectroscopy and imaging outcomes rely upon many factors; one of the most critical is the photon acquisition and processing method employed. For some types of measurements it may be crucial to acquire every single photon quickly with temporal resolution, but in other cases it is important to acquire as many photons as possible, regardless of the time information about each of them. Fluorescence Lifetime Imaging Microscopy belongs to the first case, where the information of the time of arrival of every single photon in every single pixel is fundamental in obtaining the desired information. Spectral tissue imaging belongs to the second case, where high photon density is needed in order to calculate the optical parameters necessary to build the spectral image. In both cases, the current instrumentation suffers from limitations in terms of acquisition time, duty cycle, cost, and radio-frequency interference and emission. We developed the Digital Frequency-Domain approach for photon acquisition and processing purpose using new digital technology. This approach is based on the use of photon detectors in photon counting mode, and the digital heterodyning method to acquire data which is analyzed in the frequency domain to provide the information of the time of arrival of the photons . In conjunction with the use of pulsed laser sources, this method allows the determination of the time of arrival of the photons using the harmonic content of the frequency domain analysis. The parallel digital FD design is a powerful approach that others the possibility to implement a variety of different applications in fluorescence spectroscopy and microscopy. It can be applied to fluorometry, Fluorescence Lifetime Imaging (FLIM), and Fluorescence Correlation Spectroscopy (FCS), as well as multi frequency and multi wavelength tissue imaging in compact portable medical devices. It dramatically reduces the acquisition time from the several minutes scale to the seconds scale, performs signal processing in a digital fashion avoiding RF emission and it is extremely inexpensive. This development is the result of a systematic study carried on a previous design known as the FLIMBox developed as part of a thesis of another graduate student. The extensive work done in maximizing the performance of the original FLIMBox led us to develop a new hardware solution with exciting and promising results and potential that were not possible in the previous hardware realization, where the signal harmonic content was limited by the FPGA technology. The new design permits acquisition of a much larger harmonic content of the sample response when it is excited with a pulsed light source in one single measurement using the digital mixing principle that was developed in the original design. Furthermore, we used the parallel digital FD principle to perform tissue imaging through Diffuse Optical Spectroscopy (DOS) measurements. We integrated the FLIMBox in a new system that uses a supercontinuum white laser with high brightness as a single light source and photomultipliers with large detection area, both allowing a high penetration depth with extremely low power at the sample. The parallel acquisition, achieved by using the FlimBox, decreases the time required for standard serial systems that scan through all modulation frequencies. Furthermore, the all-digital acquisition avoids analog noise, removes the analog mixer of the conventional frequency domain approach, and it does not generate radio-frequencies, normally present in current analog systems. We are able to obtain a very sensitive acquisition due to the high signal to noise ratio (S/N). The successful results obtained by utilizing digital technology in photon acquisition and processing, prompted us to extend the use of FPGA to other applications, such as phosphorescence detection. Using the FPGA concept we proposed possible solutions to outstanding problems with the current technology. In this thesis I discuss new possible scenarios where new FPGA chips are applied to spectral tissue imaging.

ISBN: 9781303135989Subjects--Topical Terms:

535387
Biomedical engineering.

Living in a digital world: features and applications of FPGA in photon detection.
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502 $a Thesis (Ph.D.)--University of California, Irvine, 2013.
520 $a Optical spectroscopy and imaging outcomes rely upon many factors; one of the most critical is the photon acquisition and processing method employed. For some types of measurements it may be crucial to acquire every single photon quickly with temporal resolution, but in other cases it is important to acquire as many photons as possible, regardless of the time information about each of them. Fluorescence Lifetime Imaging Microscopy belongs to the first case, where the information of the time of arrival of every single photon in every single pixel is fundamental in obtaining the desired information. Spectral tissue imaging belongs to the second case, where high photon density is needed in order to calculate the optical parameters necessary to build the spectral image. In both cases, the current instrumentation suffers from limitations in terms of acquisition time, duty cycle, cost, and radio-frequency interference and emission. We developed the Digital Frequency-Domain approach for photon acquisition and processing purpose using new digital technology. This approach is based on the use of photon detectors in photon counting mode, and the digital heterodyning method to acquire data which is analyzed in the frequency domain to provide the information of the time of arrival of the photons . In conjunction with the use of pulsed laser sources, this method allows the determination of the time of arrival of the photons using the harmonic content of the frequency domain analysis. The parallel digital FD design is a powerful approach that others the possibility to implement a variety of different applications in fluorescence spectroscopy and microscopy. It can be applied to fluorometry, Fluorescence Lifetime Imaging (FLIM), and Fluorescence Correlation Spectroscopy (FCS), as well as multi frequency and multi wavelength tissue imaging in compact portable medical devices. It dramatically reduces the acquisition time from the several minutes scale to the seconds scale, performs signal processing in a digital fashion avoiding RF emission and it is extremely inexpensive. This development is the result of a systematic study carried on a previous design known as the FLIMBox developed as part of a thesis of another graduate student. The extensive work done in maximizing the performance of the original FLIMBox led us to develop a new hardware solution with exciting and promising results and potential that were not possible in the previous hardware realization, where the signal harmonic content was limited by the FPGA technology. The new design permits acquisition of a much larger harmonic content of the sample response when it is excited with a pulsed light source in one single measurement using the digital mixing principle that was developed in the original design. Furthermore, we used the parallel digital FD principle to perform tissue imaging through Diffuse Optical Spectroscopy (DOS) measurements. We integrated the FLIMBox in a new system that uses a supercontinuum white laser with high brightness as a single light source and photomultipliers with large detection area, both allowing a high penetration depth with extremely low power at the sample. The parallel acquisition, achieved by using the FlimBox, decreases the time required for standard serial systems that scan through all modulation frequencies. Furthermore, the all-digital acquisition avoids analog noise, removes the analog mixer of the conventional frequency domain approach, and it does not generate radio-frequencies, normally present in current analog systems. We are able to obtain a very sensitive acquisition due to the high signal to noise ratio (S/N). The successful results obtained by utilizing digital technology in photon acquisition and processing, prompted us to extend the use of FPGA to other applications, such as phosphorescence detection. Using the FPGA concept we proposed possible solutions to outstanding problems with the current technology. In this thesis I discuss new possible scenarios where new FPGA chips are applied to spectral tissue imaging.
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