東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Characterization of primary murine a...

Kebaabetswe, Lemme P.

Linked to FindBook

Google Book

Amazon

博客來

Characterization of primary murine alveolar epithelial cells and their responses to respiratory viral infections.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterization of primary murine alveolar epithelial cells and their responses to respiratory viral infections./
Author:	Kebaabetswe, Lemme P.
Description:	158 p.
Notes:	Source: Dissertation Abstracts International, Volume: 75-09(E), Section: B.
Contained By:	Dissertation Abstracts International75-09B(E).
Subject:	Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3623190
ISBN:	9781303955150

Characterization of primary murine alveolar epithelial cells and their responses to respiratory viral infections.
Kebaabetswe, Lemme P.

Characterization of primary murine alveolar epithelial cells and their responses to respiratory viral infections. - 158 p.

Source: Dissertation Abstracts International, Volume: 75-09(E), Section: B.

Thesis (Ph.D.)--University of Idaho, 2014.

This item is not available from ProQuest Dissertations & Theses.

Alveolar epithelial cells are important targets of respiratory viral infections that cause severe diseases in the lung. The alveolar epithelium, which covers more than 99% of the large internal surface area of the lung, is composed of two morphologically distinct cell types. The alveolar type I (ATI) cells are large thin cells that function in gas exchange. The alveolar type II (ATII) cells are cuboidal and produce surfactant that is required to prevent collapse of the air spaces. ATII cells divide and trans-differentiate into ATI cells to repair damaged epithelium. Both ATI and ATII cells are infected by viruses and contribute to cytokine production during infection. The goal of this dissertation was to establish an in vitro model of murine ATI and ATII cells and determine their susceptibility and responses to infection by respiratory viruses used in murine models: influenza A virus strain PR8 and murine coronavirus, MHV-1. Isolated ATII cells were cultured to maintain an ATII phenotype as determined by expression of LBP180 or trans-differentiate into an ATI-like cell phenotype determined by expression of T1alpha. Primary cultures of either phenotype supported replication of PR8 and MHV-1. Further, both viruses induced cytokine expression by ATI cells. Expression of several cytokines and chemokines were induced by both viruses, while MHV-1 infection resulted in expression of an additional set of cytokines that were not expressed by PR8-infected cells. To help better understand the dynamics of PR8 and cellular proteins interactions, proteomics analysis using liquid chromatography-tandem mass spectrometry was examined on ATII cells. Protein profiles in PR8 infected and uninfected ATII cells were compared. Influenza disrupted various functional classes of proteins that may allow productive infection of ATII cells, altering their homeostatic functions. These data will help identify cellular proteins whose functions in the virus life cycle could be targeted for antiviral strategies. Moreover, differentiated cultures of murine alveolar epithelial cells will be critical for identifying mechanisms of viral damage to the alveolar epithelium during respiratory infections, by providing an in vitro model to perform mechanistic studies that can be correlated with in vivo studies in well-established murine models.

ISBN: 9781303955150Subjects--Topical Terms:

536250
Microbiology.

Characterization of primary murine alveolar epithelial cells and their responses to respiratory viral infections.
LDR:03351nmm a2200301 4500 001 2078440
005 20161122122605.5
008 170521s2014 ||||||||||||||||| ||eng d
020 $a 9781303955150
035 $a (MiAaPQ)AAI3623190
035 $a AAI3623190
040 $a MiAaPQ $c MiAaPQ
100 1 $a Kebaabetswe, Lemme P. $3 3194024
245 1 0 $a Characterization of primary murine alveolar epithelial cells and their responses to respiratory viral infections.
300 $a 158 p.
500 $a Source: Dissertation Abstracts International, Volume: 75-09(E), Section: B.
500 $a Adviser: Tanya Miura.
502 $a Thesis (Ph.D.)--University of Idaho, 2014.
506 $a This item is not available from ProQuest Dissertations & Theses.
520 $a Alveolar epithelial cells are important targets of respiratory viral infections that cause severe diseases in the lung. The alveolar epithelium, which covers more than 99% of the large internal surface area of the lung, is composed of two morphologically distinct cell types. The alveolar type I (ATI) cells are large thin cells that function in gas exchange. The alveolar type II (ATII) cells are cuboidal and produce surfactant that is required to prevent collapse of the air spaces. ATII cells divide and trans-differentiate into ATI cells to repair damaged epithelium. Both ATI and ATII cells are infected by viruses and contribute to cytokine production during infection. The goal of this dissertation was to establish an in vitro model of murine ATI and ATII cells and determine their susceptibility and responses to infection by respiratory viruses used in murine models: influenza A virus strain PR8 and murine coronavirus, MHV-1. Isolated ATII cells were cultured to maintain an ATII phenotype as determined by expression of LBP180 or trans-differentiate into an ATI-like cell phenotype determined by expression of T1alpha. Primary cultures of either phenotype supported replication of PR8 and MHV-1. Further, both viruses induced cytokine expression by ATI cells. Expression of several cytokines and chemokines were induced by both viruses, while MHV-1 infection resulted in expression of an additional set of cytokines that were not expressed by PR8-infected cells. To help better understand the dynamics of PR8 and cellular proteins interactions, proteomics analysis using liquid chromatography-tandem mass spectrometry was examined on ATII cells. Protein profiles in PR8 infected and uninfected ATII cells were compared. Influenza disrupted various functional classes of proteins that may allow productive infection of ATII cells, altering their homeostatic functions. These data will help identify cellular proteins whose functions in the virus life cycle could be targeted for antiviral strategies. Moreover, differentiated cultures of murine alveolar epithelial cells will be critical for identifying mechanisms of viral damage to the alveolar epithelium during respiratory infections, by providing an in vitro model to perform mechanistic studies that can be correlated with in vivo studies in well-established murine models.
590 $a School code: 0089.
650 4 $a Microbiology. $3 536250
650 4 $a Immunology. $3 611031
650 4 $a Virology. $3 642304
690 $a 0410
690 $a 0982
690 $a 0720
710 2 $a University of Idaho. $b Biology. $3 3194025
773 0 $t Dissertation Abstracts International $g 75-09B(E).
790 $a 0089
791 $a Ph.D.
792 $a 2014
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3623190