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A Phenomic Assessment of Subcellular...

Styles, Erin B.

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A Phenomic Assessment of Subcellular Morphology in Saccharomyces cerevisiae Using Synthetic Genetic Array Analysis and High-Content Screening.

Record Type:	Electronic resources : Monograph/item
Title/Author:	A Phenomic Assessment of Subcellular Morphology in Saccharomyces cerevisiae Using Synthetic Genetic Array Analysis and High-Content Screening./
Author:	Styles, Erin B.
Description:	310 p.
Notes:	Source: Dissertation Abstracts International, Volume: 77-06(E), Section: B.
Contained By:	Dissertation Abstracts International77-06B(E).
Subject:	Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3744180
ISBN:	9781339369280

A Phenomic Assessment of Subcellular Morphology in Saccharomyces cerevisiae Using Synthetic Genetic Array Analysis and High-Content Screening.
Styles, Erin B.

A Phenomic Assessment of Subcellular Morphology in Saccharomyces cerevisiae Using Synthetic Genetic Array Analysis and High-Content Screening. - 310 p.

Source: Dissertation Abstracts International, Volume: 77-06(E), Section: B.

Thesis (Ph.D.)--University of Toronto (Canada), 2015.

In this thesis, I describe the optimization and adaptation of an integrated SGA-HCS pipeline complete with a robust panel of downstream statistical metrics, to analyze subcellular phenotypes in yeast cells in the context of the yeast deletion collection (Giaever et al., 2002a; Winzeler, 1999) and the ts collection of essential gene mutants (Li et al., 2011a). I have used this experimental pipeline to closely examine the utility of both single and double mutant screening strategies for the identification of subtle subcellular phenotypes, with a special focus on the formation of DNA damage foci, and have subsequently characterized Vid22, a protein with a previously unappreciated role in the DNA damage response. I have additionally endeavored to curate known cellular and subcellular mutant morphologies into a database, using a systematic rhetoric to describe conditions and observable phenotypes in order to provide a resource and gold standard set of mutants with which to assess ongoing Marker Project screens.

ISBN: 9781339369280Subjects--Topical Terms:

530508
Genetics.

A Phenomic Assessment of Subcellular Morphology in Saccharomyces cerevisiae Using Synthetic Genetic Array Analysis and High-Content Screening.
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245 1 2 $a A Phenomic Assessment of Subcellular Morphology in Saccharomyces cerevisiae Using Synthetic Genetic Array Analysis and High-Content Screening.
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500 $a Source: Dissertation Abstracts International, Volume: 77-06(E), Section: B.
502 $a Thesis (Ph.D.)--University of Toronto (Canada), 2015.
520 $a In this thesis, I describe the optimization and adaptation of an integrated SGA-HCS pipeline complete with a robust panel of downstream statistical metrics, to analyze subcellular phenotypes in yeast cells in the context of the yeast deletion collection (Giaever et al., 2002a; Winzeler, 1999) and the ts collection of essential gene mutants (Li et al., 2011a). I have used this experimental pipeline to closely examine the utility of both single and double mutant screening strategies for the identification of subtle subcellular phenotypes, with a special focus on the formation of DNA damage foci, and have subsequently characterized Vid22, a protein with a previously unappreciated role in the DNA damage response. I have additionally endeavored to curate known cellular and subcellular mutant morphologies into a database, using a systematic rhetoric to describe conditions and observable phenotypes in order to provide a resource and gold standard set of mutants with which to assess ongoing Marker Project screens.
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