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Nuclear PTEN controls DNA repair and...

Bassi, Christian.

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Nuclear PTEN controls DNA repair and sensitivity to genotoxic stress.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Nuclear PTEN controls DNA repair and sensitivity to genotoxic stress./
Author:	Bassi, Christian.
Description:	195 p.
Notes:	Source: Dissertation Abstracts International, Volume: 77-06(E), Section: B.
Contained By:	Dissertation Abstracts International77-06B(E).
Subject:	Molecular biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3743214
ISBN:	9781339358840

Nuclear PTEN controls DNA repair and sensitivity to genotoxic stress.
Bassi, Christian.

Nuclear PTEN controls DNA repair and sensitivity to genotoxic stress. - 195 p.

Source: Dissertation Abstracts International, Volume: 77-06(E), Section: B.

Thesis (Ph.D.)--University of Toronto (Canada), 2015.

Loss of function of the phosphatase and tensin homolog (PTEN) tumor suppressor is frequently found in many human malignancies. PTEN antagonizes the Phosphatidylinositide 3-kinase (PI3K) pathway (1) by dephosphorylating the 3 position of phosphatidylinositol (3,4,5)-trisphosphate [PtdIns(3,4,5)P3] (PIP3). PIP3 serves as a second messenger whose levels in the plasma membrane are elevated following cell stimulation with growth factors, mediated by the activity of PI3Ks. Proteins containing pleckstrin homology (PH) domains physically interact with PIP3, bringing them into close proximity with other PH domain-containing proteins and facilitating further functional interactions that serve to propagate the membranous signal.

ISBN: 9781339358840Subjects--Topical Terms:

517296
Molecular biology.

Nuclear PTEN controls DNA repair and sensitivity to genotoxic stress.
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100 1 $a Bassi, Christian. $3 3193211
245 1 0 $a Nuclear PTEN controls DNA repair and sensitivity to genotoxic stress.
300 $a 195 p.
500 $a Source: Dissertation Abstracts International, Volume: 77-06(E), Section: B.
502 $a Thesis (Ph.D.)--University of Toronto (Canada), 2015.
520 $a Loss of function of the phosphatase and tensin homolog (PTEN) tumor suppressor is frequently found in many human malignancies. PTEN antagonizes the Phosphatidylinositide 3-kinase (PI3K) pathway (1) by dephosphorylating the 3 position of phosphatidylinositol (3,4,5)-trisphosphate [PtdIns(3,4,5)P3] (PIP3). PIP3 serves as a second messenger whose levels in the plasma membrane are elevated following cell stimulation with growth factors, mediated by the activity of PI3Ks. Proteins containing pleckstrin homology (PH) domains physically interact with PIP3, bringing them into close proximity with other PH domain-containing proteins and facilitating further functional interactions that serve to propagate the membranous signal.
520 $a Although the cytoplasmic role of PTEN in antagonizing PI3K signaling has been well studied, PTEN also resides in the nucleus, where its function remains poorly understood. Here, I demonstrate that SUMOylation (SUMO) of PTEN controls its nuclear localization. Consistent with its restricted cytoplasmic localization, a SUMO-deficient PTEN mutant is fully competent for regulating PI3K signaling. Upon exposure to genotoxic stress, SUMO-PTEN is rapidly excluded from the nucleus in an ATM-dependent manner, identifying a connection between these two major tumor suppressors. Further, ATM phosphorylated PTEN on threonine 398, and a PTEN mutant that cannot be phosphorylated at this position (PTEN T398A) resist nuclear exclusion following genotoxic stress. Judging by various readouts of the DNA damage response, cells lacking nuclear PTEN are hypersensitive to DNA damage and display impaired homologous recombination-mediated repair of double-strand DNA breaks. Moreover, unlike cells with Wt PTEN, PTEN-deficient cells are susceptible to killing by a combination of genotoxic stress and a small molecule inhibitor of PI3K both in vitro and in vivo. The synergistc effect of genotoxic stress and PI3K inhibition on PTEN-null cells is dependent on the simultaneous inhibition of both p110alpha and p110beta isoform. Further in vivo studies revealed that effective inhibition of PI3K pathway can be achieved with a discontinuous administration schedule in order to reduce the adverse effects associated with this treatment. My findings have considerable implications for individualizing therapy for patients with PTEN-deficient tumors.
590 $a School code: 0779.
650 4 $a Molecular biology. $3 517296
650 4 $a Cellular biology. $3 3172791
650 4 $a Biochemistry. $3 518028
650 4 $a Genetics. $3 530508
650 4 $a Oncology. $3 751006
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710 2 $a University of Toronto (Canada). $3 1017674
773 0 $t Dissertation Abstracts International $g 77-06B(E).
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791 $a Ph.D.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3743214