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MicroRNAs in the Regulation of Cellu...

Li, Haoran.

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MicroRNAs in the Regulation of Cellular Stress Responses.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	MicroRNAs in the Regulation of Cellular Stress Responses./
作者:	Li, Haoran.
面頁冊數:	202 p.
附註:	Source: Dissertation Abstracts International, Volume: 76-11(E), Section: B.
Contained By:	Dissertation Abstracts International76-11B(E).
標題:	Cellular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3709151
ISBN:	9781321846621

MicroRNAs in the Regulation of Cellular Stress Responses.
Li, Haoran.

MicroRNAs in the Regulation of Cellular Stress Responses. - 202 p.

Source: Dissertation Abstracts International, Volume: 76-11(E), Section: B.

Thesis (Ph.D.)--University of Toronto (Canada), 2015.

MicroRNAs are key regulators of cellular functions at post-transcriptional level. There is an emerging concept that microRNAs are involved in the regulation of how cells respond to changes in environment and stress conditions. Understanding the consequence of microRNA regulatory network has the potential to answer a multitude of fundamental questions. Dysregulation of these processes is associated with cancer development and drug resistance. In this dissertation, four studies are presented. The first study demonstrated that microRNA-17 targets both oncogene MDM2 and tumor suppressor gene PTEN: it suppresses glioblastoma tumor cell proliferation in favorable condition. However, when challenged by starvation or chemotherapy, it induced angiogenesis and the generation of tumor stem-like cells, and helping tumor cells survive in metabolic stress. The second study revealed the role of microRNA-17 in the regulation of chemotherapy sensitivity. By analyzing tissue samples from colorectal cancer patients, we found that microRNA-17 serves as a predictive factor of chemotherapy and prognostic factor of overall survival. Overexpression of microRNA-17 in colorectal cancer cells increased drug resistance and cell motility. The third study focused on the impact of microRNA on antitumor immune response. We found vigorous anti-melanoma immune response in microRNA-17 transgenic mice, which is characterized by CD8+ T lymphocytes infiltration. We further demonstrated the potential role of microRNA in the coordination of interaction between tumor cells and microenvironment. The fourth study examined the function of microRNA in tissue regeneration. We found an enhanced wound healing process in anti-microRNA-378 transgenic mice that have otherwise normal phenotype. Knocking down microRNA increased the expression of its targets Vimentin and Integrin beta-3, helping wound healing. Conjugated gold nanoparticle treatment delivered antisense oligos to the wound area and achieved therapeutic effect. Taken together, these studies illustrate the multiplicity of microRNA in the coordination of cellular stress responses.

ISBN: 9781321846621Subjects--Topical Terms:

3172791
Cellular biology.

MicroRNAs in the Regulation of Cellular Stress Responses.
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