東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Synthetic surfaces to probe human pl...

Wrighton, Paul J.

FindBook

Google Book

Amazon

博客來

Synthetic surfaces to probe human pluripotent stem cell fate decisions.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Synthetic surfaces to probe human pluripotent stem cell fate decisions./
作者:	Wrighton, Paul J.
面頁冊數:	188 p.
附註:	Source: Dissertation Abstracts International, Volume: 76-09(E), Section: B.
Contained By:	Dissertation Abstracts International76-09B(E).
標題:	Biochemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3689867
ISBN:	9781321695496

Synthetic surfaces to probe human pluripotent stem cell fate decisions.
Wrighton, Paul J.

Synthetic surfaces to probe human pluripotent stem cell fate decisions. - 188 p.

Source: Dissertation Abstracts International, Volume: 76-09(E), Section: B.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2015.

The field of regenerative medicine promises to restore function to failing organs and tissues in patients with debilitating degenerative diseases or injuries. One branch of the field focuses on discovering methods to induce endogenous tissues to regenerate or repair themselves. The other branch seeks to produce replacement tissues and organs in vitro to serve as a limitless source for transplants. Human pluripotent stem cells---with the ability to self-renew indefinitely in culture and differentiated into virtually any functional cell type---hold promise as source material for regenerative medicine. In order to achieve this promise, however, we must develop methods to control the proliferation and differentiation of the cells and the methods must keep them free of contaminative immunogens or pathogens. This thesis focuses on the juncture of these two goals. Initially, we developed of the first synthetic surface capable of supporting human pluripotent stem cells. This synthetic surface interacts with cell surface glycosaminoglycans, and I aimed to determine how insoluble cues influence the signaling mechanisms involved in cell fate determination. First, I developed peptide-presenting surfaces tailored to the changing adhesion needs of pluripotent stem cells as they differentiated into neural progenitor cells and motor neurons (Chapter 2). I demonstrated that cells differentiate more robustly toward endoderm and mesoderm lineages when cultured on GAG-binding surfaces. Further, I probed the molecular mechanisms involved and showed that integrin activation of Akt via integrin-linked kinase inhibits mesendoderm differentiation (Chapter 3). We also analyzed the effects of mechanical cues on stem cell fate. Human pluripotent stem cells require a surface of sufficient stiffness to maintain pluripotency. When cultured on softer surfaces, they rapidly differentiate into neurons, and we demonstrated that this process is governed by the mechanosensitive transcriptional coactivator Yes-associated protein (Chapter 4). Cumulatively, this thesis dissects and reveals mechanisms by which insoluble cues control human fate decisions. The continued research into the crosstalk between insoluble and soluble signaling mechanisms will facilitate the goals of regenerative medicine.

ISBN: 9781321695496Subjects--Topical Terms:

518028
Biochemistry.

Synthetic surfaces to probe human pluripotent stem cell fate decisions.
LDR:03202nmm a2200289 4500 001 2077426
005 20161114130312.5
008 170521s2015 ||||||||||||||||| ||eng d
020 $a 9781321695496
035 $a (MiAaPQ)AAI3689867
035 $a AAI3689867
040 $a MiAaPQ $c MiAaPQ
100 1 $a Wrighton, Paul J. $3 3192930
245 1 0 $a Synthetic surfaces to probe human pluripotent stem cell fate decisions.
300 $a 188 p.
500 $a Source: Dissertation Abstracts International, Volume: 76-09(E), Section: B.
500 $a Adviser: Laura L. Kiessling.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2015.
520 $a The field of regenerative medicine promises to restore function to failing organs and tissues in patients with debilitating degenerative diseases or injuries. One branch of the field focuses on discovering methods to induce endogenous tissues to regenerate or repair themselves. The other branch seeks to produce replacement tissues and organs in vitro to serve as a limitless source for transplants. Human pluripotent stem cells---with the ability to self-renew indefinitely in culture and differentiated into virtually any functional cell type---hold promise as source material for regenerative medicine. In order to achieve this promise, however, we must develop methods to control the proliferation and differentiation of the cells and the methods must keep them free of contaminative immunogens or pathogens. This thesis focuses on the juncture of these two goals. Initially, we developed of the first synthetic surface capable of supporting human pluripotent stem cells. This synthetic surface interacts with cell surface glycosaminoglycans, and I aimed to determine how insoluble cues influence the signaling mechanisms involved in cell fate determination. First, I developed peptide-presenting surfaces tailored to the changing adhesion needs of pluripotent stem cells as they differentiated into neural progenitor cells and motor neurons (Chapter 2). I demonstrated that cells differentiate more robustly toward endoderm and mesoderm lineages when cultured on GAG-binding surfaces. Further, I probed the molecular mechanisms involved and showed that integrin activation of Akt via integrin-linked kinase inhibits mesendoderm differentiation (Chapter 3). We also analyzed the effects of mechanical cues on stem cell fate. Human pluripotent stem cells require a surface of sufficient stiffness to maintain pluripotency. When cultured on softer surfaces, they rapidly differentiate into neurons, and we demonstrated that this process is governed by the mechanosensitive transcriptional coactivator Yes-associated protein (Chapter 4). Cumulatively, this thesis dissects and reveals mechanisms by which insoluble cues control human fate decisions. The continued research into the crosstalk between insoluble and soluble signaling mechanisms will facilitate the goals of regenerative medicine.
590 $a School code: 0262.
650 4 $a Biochemistry. $3 518028
650 4 $a Cellular biology. $3 3172791
650 4 $a Chemistry. $3 516420
690 $a 0487
690 $a 0379
690 $a 0485
710 2 $a The University of Wisconsin - Madison. $b Biochemistry-ALS. $3 2100500
773 0 $t Dissertation Abstracts International $g 76-09B(E).
790 $a 0262
791 $a Ph.D.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3689867