東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

The Role of Cardiac Neural Crest Cel...

Cavanaugh, Ann Milada.

Linked to FindBook

Google Book

Amazon

博客來

The Role of Cardiac Neural Crest Cells in Zebrafish Heart Development.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The Role of Cardiac Neural Crest Cells in Zebrafish Heart Development./
Author:	Cavanaugh, Ann Milada.
Description:	126 p.
Notes:	Source: Dissertation Abstracts International, Volume: 76-08(E), Section: B.
Contained By:	Dissertation Abstracts International76-08B(E).
Subject:	Developmental biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3688000
ISBN:	9781321660425

The Role of Cardiac Neural Crest Cells in Zebrafish Heart Development.
Cavanaugh, Ann Milada.

The Role of Cardiac Neural Crest Cells in Zebrafish Heart Development. - 126 p.

Source: Dissertation Abstracts International, Volume: 76-08(E), Section: B.

Thesis (Ph.D.)--University of California, Los Angeles, 2015.

Early stages of cardiac development are well conserved among vertebrates. However, later morphological development results in a more complicated 4 chambered heart in mammals, while fish retain a simple two chambered heart. The onset of these morphological changes coincide with contribution of cells to the heart from the second heart field (SHF). Interestingly, despite the great morphological differences in the structures derived from precursors in the SHF in mammals and zebrafish, the signals regulating SHF development are conserved. Cardiac neural crest cells (CNCCs), which also contribute to later stages of cardiac development are well studied in chick and mouse models, but very little is known about the role of CNCCs in zebrafish heart development. It is possible that as with the SHF the molecular mechanisms of CNCC development is conserved among all vertebrates. In this study I aim to better define the role of CNCCs in zebrafish and determine how well the role for CNCCs is conserved. I show that there are two waves of CNCCs which contribute to the heart in zebrafish. The first invades the developing heart tube between 24 hpf and 30 hpf and gives rise to CNC derived cardiomyocytes. CNCCs with a myocardial fate have an invasive morphology as they enter the heart, and disrupt local adhesion molecules in neighboring cells. The second wave of CNCCs migrates along aortic arch artery 6, onto the ventral aorta by 80 hpf, and ultimately invades the BA. I find that both populations of CNC derived cells persist to adulthood. These two populations are separated not only by developmental time, but also by their response to FGF signaling as they migrate to the heart. The first wave is independent of Tbx1, and FGF signaling, and the second wave relies on FGF signaling for migration to the ventral aorta and BA. Ablation of NC leads to a variety of cardiac defects including reduced heart rate, defects in myocardial maturation, development of the BA and aorta, and defects in SHF contribution to the heart, as well as a dramatic increase of bmp4 expression, and a reduction of tbx1 expression. Many of the cardiac phenotypes I observe in NC ablated embryos are similar to those reported in other species, making zebrafish an ideal model to study signaling which may be important for CNC development in vertebrates.

ISBN: 9781321660425Subjects--Topical Terms:

592588
Developmental biology.

The Role of Cardiac Neural Crest Cells in Zebrafish Heart Development.
LDR:03193nmm a2200265 4500 001 2077413
005 20161114130310.5
008 170521s2015 ||||||||||||||||| ||eng d
020 $a 9781321660425
035 $a (MiAaPQ)AAI3688000
035 $a AAI3688000
040 $a MiAaPQ $c MiAaPQ
100 1 $a Cavanaugh, Ann Milada. $3 3192916
245 1 4 $a The Role of Cardiac Neural Crest Cells in Zebrafish Heart Development.
300 $a 126 p.
500 $a Source: Dissertation Abstracts International, Volume: 76-08(E), Section: B.
500 $a Adviser: Jau-Nian Chen.
502 $a Thesis (Ph.D.)--University of California, Los Angeles, 2015.
520 $a Early stages of cardiac development are well conserved among vertebrates. However, later morphological development results in a more complicated 4 chambered heart in mammals, while fish retain a simple two chambered heart. The onset of these morphological changes coincide with contribution of cells to the heart from the second heart field (SHF). Interestingly, despite the great morphological differences in the structures derived from precursors in the SHF in mammals and zebrafish, the signals regulating SHF development are conserved. Cardiac neural crest cells (CNCCs), which also contribute to later stages of cardiac development are well studied in chick and mouse models, but very little is known about the role of CNCCs in zebrafish heart development. It is possible that as with the SHF the molecular mechanisms of CNCC development is conserved among all vertebrates. In this study I aim to better define the role of CNCCs in zebrafish and determine how well the role for CNCCs is conserved. I show that there are two waves of CNCCs which contribute to the heart in zebrafish. The first invades the developing heart tube between 24 hpf and 30 hpf and gives rise to CNC derived cardiomyocytes. CNCCs with a myocardial fate have an invasive morphology as they enter the heart, and disrupt local adhesion molecules in neighboring cells. The second wave of CNCCs migrates along aortic arch artery 6, onto the ventral aorta by 80 hpf, and ultimately invades the BA. I find that both populations of CNC derived cells persist to adulthood. These two populations are separated not only by developmental time, but also by their response to FGF signaling as they migrate to the heart. The first wave is independent of Tbx1, and FGF signaling, and the second wave relies on FGF signaling for migration to the ventral aorta and BA. Ablation of NC leads to a variety of cardiac defects including reduced heart rate, defects in myocardial maturation, development of the BA and aorta, and defects in SHF contribution to the heart, as well as a dramatic increase of bmp4 expression, and a reduction of tbx1 expression. Many of the cardiac phenotypes I observe in NC ablated embryos are similar to those reported in other species, making zebrafish an ideal model to study signaling which may be important for CNC development in vertebrates.
590 $a School code: 0031.
650 4 $a Developmental biology. $3 592588
690 $a 0758
710 2 $a University of California, Los Angeles. $b Molec, Cell,and Dev Biology. $3 3192917
773 0 $t Dissertation Abstracts International $g 76-08B(E).
790 $a 0031
791 $a Ph.D.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3688000