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The role of Hsp90 in spermatogenesis...

Ross, Robert James.

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The role of Hsp90 in spermatogenesis and the Piwi-piRNA pathway.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The role of Hsp90 in spermatogenesis and the Piwi-piRNA pathway./
作者:	Ross, Robert James.
面頁冊數:	173 p.
附註:	Source: Dissertation Abstracts International, Volume: 76-11(E), Section: B.
Contained By:	Dissertation Abstracts International76-11B(E).
標題:	Cellular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3663574
ISBN:	9781321945560

The role of Hsp90 in spermatogenesis and the Piwi-piRNA pathway.
Ross, Robert James.

The role of Hsp90 in spermatogenesis and the Piwi-piRNA pathway. - 173 p.

Source: Dissertation Abstracts International, Volume: 76-11(E), Section: B.

Thesis (Ph.D.)--Yale University, 2015.

This item is not available from ProQuest Dissertations & Theses.

Canalization refers to developmental robustness, an organism's ability to develop the same phenotype despite changes in genotype or environment. In Drosophila, we and others previously outlined two major mechanisms by which Hsp90 and the Piwi-piRNA pathway function as critical canalizers: transposon silencing which prevents development of novel genetic variation and epigenetic silencing of existing variation. Despite this headway, many details of these processes remain elusive. Here we further examine the evolutionary conservation and tissue specific characteristics of these two major mechanisms. We find that in mice Hsp90alpha and Hsp90beta function independent of the piRNA pathway; localization and expression of piRNA pathway components, piRNA biogenesis, transposable element suppression, and DNA damage as assayed by yH2A.x foci formation remain unchanged despite complete knock-out of Hsp90alpha and chemical inhibition of both Hsp90alpha and Hsp90beta. Prompted by this result, we reexamine the function of Hsp90 in Drosophila. We show that the hsp83scratch mutant is competent to produce piRNAs and suppress transposable elements in the Drosophila ovary. Hsp83scratch testes show limited derepression of transposable elements which is likely due to depletion of a small number of piRNAs rather than global collapse of piRNA production. Instead, we identify an essential and conserved function of murine Hsp90alpha/beta in spermatogenesis. Genetic depletion of Hsp90alpha results in meiotic arrest of spermatogenesis and pharmacologic depletion of both Hsp90alpha/beta results in near total loss of the germline in an irreversible manner. Together, our results show that Hsp90 has a conserved function in male germline maintenance independent of the Piwi-piRNA pathway.

ISBN: 9781321945560Subjects--Topical Terms:

3172791
Cellular biology.

The role of Hsp90 in spermatogenesis and the Piwi-piRNA pathway.
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520 $a Canalization refers to developmental robustness, an organism's ability to develop the same phenotype despite changes in genotype or environment. In Drosophila, we and others previously outlined two major mechanisms by which Hsp90 and the Piwi-piRNA pathway function as critical canalizers: transposon silencing which prevents development of novel genetic variation and epigenetic silencing of existing variation. Despite this headway, many details of these processes remain elusive. Here we further examine the evolutionary conservation and tissue specific characteristics of these two major mechanisms. We find that in mice Hsp90alpha and Hsp90beta function independent of the piRNA pathway; localization and expression of piRNA pathway components, piRNA biogenesis, transposable element suppression, and DNA damage as assayed by yH2A.x foci formation remain unchanged despite complete knock-out of Hsp90alpha and chemical inhibition of both Hsp90alpha and Hsp90beta. Prompted by this result, we reexamine the function of Hsp90 in Drosophila. We show that the hsp83scratch mutant is competent to produce piRNAs and suppress transposable elements in the Drosophila ovary. Hsp83scratch testes show limited derepression of transposable elements which is likely due to depletion of a small number of piRNAs rather than global collapse of piRNA production. Instead, we identify an essential and conserved function of murine Hsp90alpha/beta in spermatogenesis. Genetic depletion of Hsp90alpha results in meiotic arrest of spermatogenesis and pharmacologic depletion of both Hsp90alpha/beta results in near total loss of the germline in an irreversible manner. Together, our results show that Hsp90 has a conserved function in male germline maintenance independent of the Piwi-piRNA pathway.
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