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The organization of mitochondrial qu...

Devireddy, Swathi.

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The organization of mitochondrial quality control and life cycle in the nervous system in vivo in the absence of PINK1.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The organization of mitochondrial quality control and life cycle in the nervous system in vivo in the absence of PINK1./
作者:	Devireddy, Swathi.
面頁冊數:	84 p.
附註:	Source: Dissertation Abstracts International, Volume: 77-08(E), Section: B.
Contained By:	Dissertation Abstracts International77-08B(E).
標題:	Cellular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10096843
ISBN:	9781339609300

The organization of mitochondrial quality control and life cycle in the nervous system in vivo in the absence of PINK1.
Devireddy, Swathi.

The organization of mitochondrial quality control and life cycle in the nervous system in vivo in the absence of PINK1. - 84 p.

Source: Dissertation Abstracts International, Volume: 77-08(E), Section: B.

Thesis (Ph.D.)--Purdue University, 2015.

Maintenance of healthy mitochondria is crucial in cells, such as neurons, with high metabolic demands, and dysfunctional mitochondria are thought to be selectively degraded. Studies of chemically uncoupled cells have implicated PINK1 mitochondrial kinase, and Parkin E3 ubiquitin ligase in targeting depolarized mitochondria for degradation. However, the role of the PINK1/Parkin pathway in mitochondrial turnover is unclear in the nervous system under normal physiological conditions, and we understand little about the changes that occur in the mitochondrial life cycle when turnover is disrupted. Here, I evaluated the nature, location and regulation of quality control in vivo using quantitative measurements of mitochondria in Drosophila nervous system, with deletion and overexpression of genes in the PINK1/Parkin pathway. I tested the hypotheses that impairment of mitochondrial quality control via suppression of PINK1 function should produce failures of turnover, accumulation of senescent mitochondria in the axon, defects in mitochondrial traffic, and a significant shift in the mitochondrial fission-fusion steady state. Although mitochondrial membrane potential was diminished by PINK1 deletion, I did not observe the predicted increases in mitochondrial density or length in axons. Loss of PINK1 also produced specific, directionally-balanced defects in mitochondrial transport, without altering the balance between stationary and moving mitochondria. Somatic mitochondrial morphology was also compromised. These results strongly circumscribe the possible mechanisms of PINK1 action in the mitochondrial life cycle, and also raise the possibility that mitochondrial turnover events that occur in cultured embryonic axons might be restricted to the cell body in vivo, in the intact nervous system.

ISBN: 9781339609300Subjects--Topical Terms:

3172791
Cellular biology.

The organization of mitochondrial quality control and life cycle in the nervous system in vivo in the absence of PINK1.
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