東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Viruses and metals in ocean food web...

Cunningham, Brady Robert.

FindBook

Google Book

Amazon

博客來

Viruses and metals in ocean food webs: Top-down and bottom-up effects of marine viruses and trace elements on marine picophytoplankton.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Viruses and metals in ocean food webs: Top-down and bottom-up effects of marine viruses and trace elements on marine picophytoplankton./
作者:	Cunningham, Brady Robert.
面頁冊數:	112 p.
附註:	Source: Dissertation Abstracts International, Volume: 77-06(E), Section: B.
Contained By:	Dissertation Abstracts International77-06B(E).
標題:	Aquatic sciences. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10008830
ISBN:	9781339455341

Viruses and metals in ocean food webs: Top-down and bottom-up effects of marine viruses and trace elements on marine picophytoplankton.
Cunningham, Brady Robert.

Viruses and metals in ocean food webs: Top-down and bottom-up effects of marine viruses and trace elements on marine picophytoplankton. - 112 p.

Source: Dissertation Abstracts International, Volume: 77-06(E), Section: B.

Thesis (Ph.D.)--University of South Carolina, 2015.

Viruses are the most numerous biological entities in the ocean, playing a key role in microbial ecology and biogeochemical cycling. In order to replicate, viruses must first infect a host and then use the host's cellular machinery to produce new viral particles. Some of the most abundant viral hosts in the subtropical open ocean are picophytoplankton. However, picophytoplankton growth is typically constrained by nutrient availability. In many regions of the ocean, iron (Fe) is only available in very low concentrations causing growth-limitation of picophytoplankton. Interestingly, the interactions between Fe-limitation and viral infection of picophytoplankton have not been extensively studied. This dissertation adds to the field by producing novel interdisciplinary research between chemical oceanography and viral ecology. The first project in this dissertation describes a new method to count DNA-stained viruses, which is drastically less expensive than commonly used methods. The new method uses epifluorescence microscopy to enumerate fluorescently stained viruses. The virus sample is combined with a known concentration of silica beads and wet mounted directly onto a slide. Viral concentration is then determined by the relative abundance of viruses and silica beads in the sample. The second project describes one-step growth curves of viruses Syn9, S-SM1, and S-PM2 infecting host Synechococcus WH7803 grown under both nutrient-replete and Fe-limited conditions. The results indicate that for these virus strains, decreased host growth rate from Fe-limitation seems to have no effect on viral reproduction. The third project presents data on the internal cellular concentrations of nutrients and trace elements when picophytoplankton are grown under varying conditions. Determining internal cellular concentrations are important when attempting to understand what nutrients are absorbed and then released by the host during viral infection and lysis. We find that Fe-limitation of cyanobacteria cells elicits a similar response to that of P-limitation, causing an overall decrease in cellular P concentration. Overall, this dissertation provides a more complete understanding of differences in viral and cellular production of Fe-limited hosts and suggests a possible role for Fe in the efficiency of the marine microbial loop, carbon, and nitrogen cycle.

ISBN: 9781339455341Subjects--Topical Terms:

3174300
Aquatic sciences.

Viruses and metals in ocean food webs: Top-down and bottom-up effects of marine viruses and trace elements on marine picophytoplankton.
LDR:03327nmm a2200277 4500 001 2074671
005 20161020134815.5
008 170521s2015 ||||||||||||||||| ||eng d
020 $a 9781339455341
035 $a (MiAaPQ)AAI10008830
035 $a AAI10008830
040 $a MiAaPQ $c MiAaPQ
100 1 $a Cunningham, Brady Robert. $3 3190008
245 1 0 $a Viruses and metals in ocean food webs: Top-down and bottom-up effects of marine viruses and trace elements on marine picophytoplankton.
300 $a 112 p.
500 $a Source: Dissertation Abstracts International, Volume: 77-06(E), Section: B.
500 $a Advisers: Seth John; Tammi Richardson.
502 $a Thesis (Ph.D.)--University of South Carolina, 2015.
520 $a Viruses are the most numerous biological entities in the ocean, playing a key role in microbial ecology and biogeochemical cycling. In order to replicate, viruses must first infect a host and then use the host's cellular machinery to produce new viral particles. Some of the most abundant viral hosts in the subtropical open ocean are picophytoplankton. However, picophytoplankton growth is typically constrained by nutrient availability. In many regions of the ocean, iron (Fe) is only available in very low concentrations causing growth-limitation of picophytoplankton. Interestingly, the interactions between Fe-limitation and viral infection of picophytoplankton have not been extensively studied. This dissertation adds to the field by producing novel interdisciplinary research between chemical oceanography and viral ecology. The first project in this dissertation describes a new method to count DNA-stained viruses, which is drastically less expensive than commonly used methods. The new method uses epifluorescence microscopy to enumerate fluorescently stained viruses. The virus sample is combined with a known concentration of silica beads and wet mounted directly onto a slide. Viral concentration is then determined by the relative abundance of viruses and silica beads in the sample. The second project describes one-step growth curves of viruses Syn9, S-SM1, and S-PM2 infecting host Synechococcus WH7803 grown under both nutrient-replete and Fe-limited conditions. The results indicate that for these virus strains, decreased host growth rate from Fe-limitation seems to have no effect on viral reproduction. The third project presents data on the internal cellular concentrations of nutrients and trace elements when picophytoplankton are grown under varying conditions. Determining internal cellular concentrations are important when attempting to understand what nutrients are absorbed and then released by the host during viral infection and lysis. We find that Fe-limitation of cyanobacteria cells elicits a similar response to that of P-limitation, causing an overall decrease in cellular P concentration. Overall, this dissertation provides a more complete understanding of differences in viral and cellular production of Fe-limited hosts and suggests a possible role for Fe in the efficiency of the marine microbial loop, carbon, and nitrogen cycle.
590 $a School code: 0202.
650 4 $a Aquatic sciences. $3 3174300
650 4 $a Environmental science. $3 677245
690 $a 0792
690 $a 0768
710 2 $a University of South Carolina. $b Marine Science. $3 1019496
773 0 $t Dissertation Abstracts International $g 77-06B(E).
790 $a 0202
791 $a Ph.D.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10008830