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A non-invasive fluorescence-based ox...

Buchapudi, Koutilya Reddy.

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A non-invasive fluorescence-based oxygen sensor and platform for studying cell responses to metabolic agents in real-time.

Record Type:	Electronic resources : Monograph/item
Title/Author:	A non-invasive fluorescence-based oxygen sensor and platform for studying cell responses to metabolic agents in real-time./
Author:	Buchapudi, Koutilya Reddy.
Description:	93 p.
Notes:	Source: Dissertation Abstracts International, Volume: 77-04(E), Section: B.
Contained By:	Dissertation Abstracts International77-04B(E).
Subject:	Biomedical engineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3664523
ISBN:	9781339283135

A non-invasive fluorescence-based oxygen sensor and platform for studying cell responses to metabolic agents in real-time.
Buchapudi, Koutilya Reddy.

A non-invasive fluorescence-based oxygen sensor and platform for studying cell responses to metabolic agents in real-time. - 93 p.

Source: Dissertation Abstracts International, Volume: 77-04(E), Section: B.

Thesis (Ph.D.)--Louisiana Tech University, 2015.

A fluorescence-based sensor in a transverse flow/stop measurement platform has been developed to determine real-time changes in oxygen consumption rates for cell metabolic studies. The oxygen sensitive fluorophore platinum octaethylporphyrin was embedded in a cellulose acetate matrix and affixed to a fiber optic bundle, which provided for transmission of the excitation and emission wavelengths of the film. The fiber optic bundle was sealed in a sensor head that can be used in standard 24-well plates common to research labs. The utility of the sensor and sensing platform were determined by measuring the changes in oxygen consumption rates of Candida albicans during 90/30 s flow/stop cycles. Exposure of these cells to metabolic antagonists and an enhancer showed the expected decrease and increase in oxygen consumption rates in real time. The applicability of the platform to biological studies is illustrated by determination of synergistic activities between antifungal drugs and fluoride exposure in Candida albicans. The robustness of the fluorophore film is demonstrated by perfusion with different media and analyte conditions in the absence of cells. For stop cycle time intervals less than 1 minute the sensor exhibited a rapid and fairly linear change in fluorescence intensity to changing oxygen concentrations in the measurement chamber. Flow cycle fluorescence intensities were used as a baseline correction for treating the stop cycle fluorescence peaks.

ISBN: 9781339283135Subjects--Topical Terms:

535387
Biomedical engineering.

A non-invasive fluorescence-based oxygen sensor and platform for studying cell responses to metabolic agents in real-time.
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245 1 2 $a A non-invasive fluorescence-based oxygen sensor and platform for studying cell responses to metabolic agents in real-time.
300 $a 93 p.
500 $a Source: Dissertation Abstracts International, Volume: 77-04(E), Section: B.
500 $a Adviser: Sven Eklund.
502 $a Thesis (Ph.D.)--Louisiana Tech University, 2015.
520 $a A fluorescence-based sensor in a transverse flow/stop measurement platform has been developed to determine real-time changes in oxygen consumption rates for cell metabolic studies. The oxygen sensitive fluorophore platinum octaethylporphyrin was embedded in a cellulose acetate matrix and affixed to a fiber optic bundle, which provided for transmission of the excitation and emission wavelengths of the film. The fiber optic bundle was sealed in a sensor head that can be used in standard 24-well plates common to research labs. The utility of the sensor and sensing platform were determined by measuring the changes in oxygen consumption rates of Candida albicans during 90/30 s flow/stop cycles. Exposure of these cells to metabolic antagonists and an enhancer showed the expected decrease and increase in oxygen consumption rates in real time. The applicability of the platform to biological studies is illustrated by determination of synergistic activities between antifungal drugs and fluoride exposure in Candida albicans. The robustness of the fluorophore film is demonstrated by perfusion with different media and analyte conditions in the absence of cells. For stop cycle time intervals less than 1 minute the sensor exhibited a rapid and fairly linear change in fluorescence intensity to changing oxygen concentrations in the measurement chamber. Flow cycle fluorescence intensities were used as a baseline correction for treating the stop cycle fluorescence peaks.
590 $a School code: 0109.
650 4 $a Biomedical engineering. $3 535387
650 4 $a Optics. $3 517925
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773 0 $t Dissertation Abstracts International $g 77-04B(E).
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856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3664523