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Decellularized porcine bone marrow-d...

Mueller, Laura Mara.

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Decellularized porcine bone marrow-derived extracellular matrix supports in vitro cultivation of mouse mesenchymal stem cells.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Decellularized porcine bone marrow-derived extracellular matrix supports in vitro cultivation of mouse mesenchymal stem cells./
Author:	Mueller, Laura Mara.
Description:	103 p.
Notes:	Source: Masters Abstracts International, Volume: 54-05.
Contained By:	Masters Abstracts International54-05(E).
Subject:	Biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1526440
ISBN:	9781321795639

Decellularized porcine bone marrow-derived extracellular matrix supports in vitro cultivation of mouse mesenchymal stem cells.
Mueller, Laura Mara.

Decellularized porcine bone marrow-derived extracellular matrix supports in vitro cultivation of mouse mesenchymal stem cells. - 103 p.

Source: Masters Abstracts International, Volume: 54-05.

Thesis (M.S.)--University of Houston-Clear Lake, 2015.

Mesenchymal stem cells (MSCs) are multipotent adult stem cells, typically found in the bone marrow (BM). They have become of great interest due to their capacity to differentiate into a variety of cell types and their ability to modulate the immune response. However, therapeutic applications require large numbers of cells; therefore, MSCs have to be expanded in vitro. Despite recognizable success, the lack of a suitable substrate for MSCs that would support proliferation while maintaining an undifferentiated state has hampered the use of MSCs for important applications. A profound influence on determining stem cell fate, either towards self-renewal or differentiation into a specific cell type, is the extracellular matrix (ECM)--the cell-free environment surrounding cells. Researchers have been developing cell culture substrates composed of ECM components or tried to reconstruct the ECM in vitro. Since these approaches showed limited improvements, the purpose of this research is to investigate the use of minimally altered, decellularized BM-derived ECM as an effective cell culture substrate for mouse MSCs. In the present study, it was found that BM-smeared cultivation plates support in vitro cultivation of MSCs while maintaining a homogenous, undifferentiated and genomically stable cell population. Analyses of BM smear composition demonstrate the presence of major BM ECM proteins and glycosaminoglycans (GAGs). Furthermore, double immunolabeling indicates a co-localization between the ECM protein fibronectin and MSCs. The results of this research will establish a BM-derived, lyophilized ECM substrate with application potential in high fidelity cultivation techniques for stem cells and provide new insight into the interaction between cells and ECM.

ISBN: 9781321795639Subjects--Topical Terms:

522710
Biology.

Decellularized porcine bone marrow-derived extracellular matrix supports in vitro cultivation of mouse mesenchymal stem cells.
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245 1 0 $a Decellularized porcine bone marrow-derived extracellular matrix supports in vitro cultivation of mouse mesenchymal stem cells.
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500 $a Source: Masters Abstracts International, Volume: 54-05.
500 $a Adviser: Richard Puzdrowski.
502 $a Thesis (M.S.)--University of Houston-Clear Lake, 2015.
520 $a Mesenchymal stem cells (MSCs) are multipotent adult stem cells, typically found in the bone marrow (BM). They have become of great interest due to their capacity to differentiate into a variety of cell types and their ability to modulate the immune response. However, therapeutic applications require large numbers of cells; therefore, MSCs have to be expanded in vitro. Despite recognizable success, the lack of a suitable substrate for MSCs that would support proliferation while maintaining an undifferentiated state has hampered the use of MSCs for important applications. A profound influence on determining stem cell fate, either towards self-renewal or differentiation into a specific cell type, is the extracellular matrix (ECM)--the cell-free environment surrounding cells. Researchers have been developing cell culture substrates composed of ECM components or tried to reconstruct the ECM in vitro. Since these approaches showed limited improvements, the purpose of this research is to investigate the use of minimally altered, decellularized BM-derived ECM as an effective cell culture substrate for mouse MSCs. In the present study, it was found that BM-smeared cultivation plates support in vitro cultivation of MSCs while maintaining a homogenous, undifferentiated and genomically stable cell population. Analyses of BM smear composition demonstrate the presence of major BM ECM proteins and glycosaminoglycans (GAGs). Furthermore, double immunolabeling indicates a co-localization between the ECM protein fibronectin and MSCs. The results of this research will establish a BM-derived, lyophilized ECM substrate with application potential in high fidelity cultivation techniques for stem cells and provide new insight into the interaction between cells and ECM.
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