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Generating CRISPR-Cas9 genome-engine...

McManus, Sean J.

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Generating CRISPR-Cas9 genome-engineered human embryonic stem cells to model a genetic mechanism of asthma.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Generating CRISPR-Cas9 genome-engineered human embryonic stem cells to model a genetic mechanism of asthma./
Author:	McManus, Sean J.
Description:	57 p.
Notes:	Source: Masters Abstracts International, Volume: 54-05.
Contained By:	Masters Abstracts International54-05(E).
Subject:	Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1591792
ISBN:	9781321837445

Generating CRISPR-Cas9 genome-engineered human embryonic stem cells to model a genetic mechanism of asthma.
McManus, Sean J.

Generating CRISPR-Cas9 genome-engineered human embryonic stem cells to model a genetic mechanism of asthma. - 57 p.

Source: Masters Abstracts International, Volume: 54-05.

Thesis (M.S.)--Boston University, 2015.

This item must not be sold to any third party vendors.

Asthma is a major public health epidemic that presents a heavy burden on those who suffer from the disease. Little is currently understood about the genetic signature that distinguishes one type of asthma from another. Recently, the single nucleotide polymorphism (SNP) rs968567 was found to have a high degree of association in asthmatic patients (Sharma et al., 2014). This particular SNP is in the promoter region of the FADS2 gene that synthesizes the enzyme delta-6-desaturase (D6D). D6D mediates the formation of pro-inflammatory factors that lead to exacerbation of asthmatic symptoms. We engineered a novel, customized CRISPR-Cas9 construct to induce the SNP rs968567 in the HUES9 human embryonic stem cell (hESC) line. Our results show success in generating the custom CRISPR-Cas9 construct for use in stem cells, while efficiency in expressing the desired mutation in our cell line is currently being optimized. Disease modeling in the genomic era of medicine provides an opportunity for the development of personalized medical treatment. Future projects aim to differentiate stem cell lines edited with our CRISPR-Cas9 construct to lung progenitor cells to study the cellular phenotype of this mutation in context of asthma pathogenesis.

ISBN: 9781321837445Subjects--Topical Terms:

530508
Genetics.

Generating CRISPR-Cas9 genome-engineered human embryonic stem cells to model a genetic mechanism of asthma.
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245 1 0 $a Generating CRISPR-Cas9 genome-engineered human embryonic stem cells to model a genetic mechanism of asthma.
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500 $a Source: Masters Abstracts International, Volume: 54-05.
500 $a Advisers: Jean L. Spencer; Paul H. Lerou.
502 $a Thesis (M.S.)--Boston University, 2015.
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520 $a Asthma is a major public health epidemic that presents a heavy burden on those who suffer from the disease. Little is currently understood about the genetic signature that distinguishes one type of asthma from another. Recently, the single nucleotide polymorphism (SNP) rs968567 was found to have a high degree of association in asthmatic patients (Sharma et al., 2014). This particular SNP is in the promoter region of the FADS2 gene that synthesizes the enzyme delta-6-desaturase (D6D). D6D mediates the formation of pro-inflammatory factors that lead to exacerbation of asthmatic symptoms. We engineered a novel, customized CRISPR-Cas9 construct to induce the SNP rs968567 in the HUES9 human embryonic stem cell (hESC) line. Our results show success in generating the custom CRISPR-Cas9 construct for use in stem cells, while efficiency in expressing the desired mutation in our cell line is currently being optimized. Disease modeling in the genomic era of medicine provides an opportunity for the development of personalized medical treatment. Future projects aim to differentiate stem cell lines edited with our CRISPR-Cas9 construct to lung progenitor cells to study the cellular phenotype of this mutation in context of asthma pathogenesis.
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