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Humoral immune response of great lak...

Millard, Elena Virginia.

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Humoral immune response of great lakes fishes to viral hemorrhagic septicemia virus genotype IVb.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Humoral immune response of great lakes fishes to viral hemorrhagic septicemia virus genotype IVb./
Author:	Millard, Elena Virginia.
Description:	219 p.
Notes:	Source: Dissertation Abstracts International, Volume: 75-04(E), Section: B.
Contained By:	Dissertation Abstracts International75-04B(E).
Subject:	Aquatic sciences. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3606470
ISBN:	9781303636080

Humoral immune response of great lakes fishes to viral hemorrhagic septicemia virus genotype IVb.
Millard, Elena Virginia.

Humoral immune response of great lakes fishes to viral hemorrhagic septicemia virus genotype IVb. - 219 p.

Source: Dissertation Abstracts International, Volume: 75-04(E), Section: B.

Thesis (Ph.D.)--Michigan State University, 2013.

This item must not be sold to any third party vendors.

Viral hemorrhagic septicemia virus genotype IVb (VHSV-IVb) is a recently emerged pathogen of Great Lakes fishes in North America. Following initial fish kills in Lake St. Clair, Michigan (MI) in 2006, VHSV-IVb went undetected for nearly three years there until another die-off ensued in the summer of 2009. This prompted questions regarding host immunity to the virus as well as the usefulness of the virus isolation assay as a surveillance tool. Herein, I describe evidence of humoral immunity to VHSV-IVb in several populations of wild fishes and the development of antibodies by muskellunge ( Esox masquinongy) following experimental challenge and vaccination. Using a 50% plaque neutralization test (PNT) and a competitive enzyme-linked immunosorbent assay (cELISA), antibodies were detected in Lake St. Clair fish during all sampling years between 2004-2011 (six summers). In contrast, VHS virus was only detected during the summer of 2006 and 2009, and in both cases, fish were sampled during or immediately following mortality events. Neutralizing (PNT) and/or binding (cELISA) antibodies were detected in 5 of 13 fish species including muskellunge, northern pike (E. lucius), freshwater drum (Aplodinotus grunniens), smallmouth bass (Micropterus dolomieu) and channel catfish (Ictalurus punctatus). The greatest overall seroprevalence and antibody titers were detected in muskellunge. Antibodies were also detected by cELISA in muskellunge from Lower Fox River/Green Bay, Wisconsin and from Thornapple Lake, Michigan.

ISBN: 9781303636080Subjects--Topical Terms:

3174300
Aquatic sciences.

Humoral immune response of great lakes fishes to viral hemorrhagic septicemia virus genotype IVb.
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100 1 $a Millard, Elena Virginia. $3 3175819
245 1 0 $a Humoral immune response of great lakes fishes to viral hemorrhagic septicemia virus genotype IVb.
300 $a 219 p.
500 $a Source: Dissertation Abstracts International, Volume: 75-04(E), Section: B.
500 $a Adviser: Mohamed Faisal.
502 $a Thesis (Ph.D.)--Michigan State University, 2013.
506 $a This item must not be sold to any third party vendors.
520 $a Viral hemorrhagic septicemia virus genotype IVb (VHSV-IVb) is a recently emerged pathogen of Great Lakes fishes in North America. Following initial fish kills in Lake St. Clair, Michigan (MI) in 2006, VHSV-IVb went undetected for nearly three years there until another die-off ensued in the summer of 2009. This prompted questions regarding host immunity to the virus as well as the usefulness of the virus isolation assay as a surveillance tool. Herein, I describe evidence of humoral immunity to VHSV-IVb in several populations of wild fishes and the development of antibodies by muskellunge ( Esox masquinongy) following experimental challenge and vaccination. Using a 50% plaque neutralization test (PNT) and a competitive enzyme-linked immunosorbent assay (cELISA), antibodies were detected in Lake St. Clair fish during all sampling years between 2004-2011 (six summers). In contrast, VHS virus was only detected during the summer of 2006 and 2009, and in both cases, fish were sampled during or immediately following mortality events. Neutralizing (PNT) and/or binding (cELISA) antibodies were detected in 5 of 13 fish species including muskellunge, northern pike (E. lucius), freshwater drum (Aplodinotus grunniens), smallmouth bass (Micropterus dolomieu) and channel catfish (Ictalurus punctatus). The greatest overall seroprevalence and antibody titers were detected in muskellunge. Antibodies were also detected by cELISA in muskellunge from Lower Fox River/Green Bay, Wisconsin and from Thornapple Lake, Michigan.
520 $a Muskellunge experimentally infected with VHSV-IVb developed neutralizing antibodies by 5 -- 7 weeks (385 -- 539 degree days) post-challenge. The development of neutralizing antibodies corresponded to a decrease in virus presence and titers in sera, indicating their role in limiting VHSV-IVb infection. A reduced neutralizing antibody response was mounted by fish exposed with the lowest dose of the virus, suggesting a threshold level of infection is necessary for induction. In surviving fish, neutralizing antibodies were detectable for a longer period of time after infection then was the virus in tissues (assessed in a parallel study). To better understand host immune response in this species, a DNA vaccine encoding the glycoprotein (G) gene of VHSV-IVb was developed. DNA vaccination primed the adaptive immune response to respond to VHSV-IVb as evident by high levels of neutralizing antibodies in 100% of surviving muskellunge by only 4 weeks (310 degree days) after challenge. Compared to plasmid control fish, pVHSivb-G-vaccinated fish were significantly protected (relative percent survival = 45.2%) following lethal virus challenge 7 weeks (539 degree days) postvaccination. Surviving fish from the pVHSivb-G vaccinated group also had significantly lower infection prevalence and tissue viral loads compared to control fish. The cELISA, developed as part of this dissertation, and the PNT are non-lethal immunological tools that will find multiple applications in future studies for assessment of adaptive immunity to VHSV-IVb.
590 $a School code: 0128.
650 4 $a Aquatic sciences. $3 3174300
650 4 $a Virology. $3 642304
650 4 $a Veterinary science. $3 3172798
650 4 $a Immunology. $3 611031
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710 2 $a Michigan State University. $b Pathobiology - Doctor of Philosophy. $3 3175820
773 0 $t Dissertation Abstracts International $g 75-04B(E).
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856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3606470