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Sablefish (Anoplopoma fimbria) sperm...

Immerman, Douglas A.

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Sablefish (Anoplopoma fimbria) sperm: The physiology of activation and the development of a cryopreservation protocol.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Sablefish (Anoplopoma fimbria) sperm: The physiology of activation and the development of a cryopreservation protocol./
作者:	Immerman, Douglas A.
面頁冊數:	53 p.
附註:	Source: Masters Abstracts International, Volume: 52-06.
Contained By:	Masters Abstracts International52-06(E).
標題:	Aquatic sciences. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1554949
ISBN:	9781303864247

Sablefish (Anoplopoma fimbria) sperm: The physiology of activation and the development of a cryopreservation protocol.
Immerman, Douglas A.

Sablefish (Anoplopoma fimbria) sperm: The physiology of activation and the development of a cryopreservation protocol. - 53 p.

Source: Masters Abstracts International, Volume: 52-06.

Thesis (Master's)--University of Washington, 2014.

This item is not available from ProQuest Dissertations & Theses.

Sablefish (Anoplopoma fimbria) is a deep-water marine species in the Pacific Ocean with high commercial value. Limited information is available about male reproductive development and sperm physiology in sablefish or how to optimize fertilization and sperm storage in this species for aquaculture. In the present study the conditions required for sablefish sperm activation were investigated and an optimized regime for cryopreservation of sablefish sperm was developed.

ISBN: 9781303864247Subjects--Topical Terms:

3174300
Aquatic sciences.

Sablefish (Anoplopoma fimbria) sperm: The physiology of activation and the development of a cryopreservation protocol.
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245 1 0 $a Sablefish (Anoplopoma fimbria) sperm: The physiology of activation and the development of a cryopreservation protocol.
300 $a 53 p.
500 $a Source: Masters Abstracts International, Volume: 52-06.
500 $a Adviser: Steven Roberts.
502 $a Thesis (Master's)--University of Washington, 2014.
506 $a This item is not available from ProQuest Dissertations & Theses.
506 $a This item must not be sold to any third party vendors.
506 $a This item must not be added to any third party search indexes.
520 $a Sablefish (Anoplopoma fimbria) is a deep-water marine species in the Pacific Ocean with high commercial value. Limited information is available about male reproductive development and sperm physiology in sablefish or how to optimize fertilization and sperm storage in this species for aquaculture. In the present study the conditions required for sablefish sperm activation were investigated and an optimized regime for cryopreservation of sablefish sperm was developed.
520 $a In all experiments, the assessment of sablefish sperm was conducted using a computer assisted sperm analysis (CASA) system that determined the velocity and linearity of motile sperm as well as the percent motility. A modified Cortland's solution was used as an extender in all activation and cryopreservation tests. Sperm were activated with ionic (NaCl, KCl, MgSO4 and CaCl 2) and non-ionic (urea and glucose) solutions of varying osmolalities. KCl and NaCl activated sperm at the lowest osmolalities, followed by MgSO 4, urea, glucose, and CaCl2. Compared to NaCl and KCl, non-ionic solutions required higher osmolalities for activation. The results indicate that the primary stimulus for sablefish sperm activation is increased osmolality and not the presence/absence of a specific ion.
520 $a The effects of several cryoprotectants including dimethyl sulfoxide, propylene glycol and glycerol at two concentrations (5% and 10%) and three freezing rates (-2.5, -5 and -7.5 °C/min) were tested on the cryopreservation of sperm using a Crysalys cryogenic programmable freezing system. There were differences observed in the motility maintained following freezing between all cryoprotectants, but the highest motility after freezing was observed with 10% dimethyl sulfoxide at all freezing rates.
590 $a School code: 0250.
650 4 $a Aquatic sciences. $3 3174300
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773 0 $t Masters Abstracts International $g 52-06(E).
790 $a 0250
791 $a Master's
792 $a 2014
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1554949