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Conversion of woody biomass to polyh...

Dai, Jing.

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Conversion of woody biomass to polyhydroxybutyrate.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Conversion of woody biomass to polyhydroxybutyrate./
Author:	Dai, Jing.
Description:	161 p.
Notes:	Source: Dissertation Abstracts International, Volume: 75-11(E), Section: B.
Contained By:	Dissertation Abstracts International75-11B(E).
Subject:	Plastics Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3627719
ISBN:	9781321031478

Conversion of woody biomass to polyhydroxybutyrate.
Dai, Jing.

Conversion of woody biomass to polyhydroxybutyrate. - 161 p.

Source: Dissertation Abstracts International, Volume: 75-11(E), Section: B.

Thesis (Ph.D.)--University of Idaho, 2014.

This project focused on the conversion of wood to the bioplastic polyhydroxybutyrate (PHB) that was successfully achieved. The first step of the conversion of wood involved a hot-water pretreatment process followed by enzymatic hydrolysis to release the monosaccharides (hydrolysates) from the lignocellulosic structure. The process was optimized using a response surface methodology (RSM). This method was proved to be efficient to convert 95% of total carbohydrates into monosaccharides (sugars) which also generated limited amount of fermentation inhibitors such as acetic acid (<1.5 g/L), furfural (<0.1 g/L) and hydroxymethylfufural (HMF) (<0.1 g/L).

ISBN: 9781321031478Subjects--Topical Terms:

1023683
Plastics Technology.

Conversion of woody biomass to polyhydroxybutyrate.
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020 $a 9781321031478
035 $a (MiAaPQ)AAI3627719
035 $a AAI3627719
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100 1 $a Dai, Jing. $3 1676916
245 1 0 $a Conversion of woody biomass to polyhydroxybutyrate.
300 $a 161 p.
500 $a Source: Dissertation Abstracts International, Volume: 75-11(E), Section: B.
500 $a Adviser: Armando G. McDonald.
502 $a Thesis (Ph.D.)--University of Idaho, 2014.
520 $a This project focused on the conversion of wood to the bioplastic polyhydroxybutyrate (PHB) that was successfully achieved. The first step of the conversion of wood involved a hot-water pretreatment process followed by enzymatic hydrolysis to release the monosaccharides (hydrolysates) from the lignocellulosic structure. The process was optimized using a response surface methodology (RSM). This method was proved to be efficient to convert 95% of total carbohydrates into monosaccharides (sugars) which also generated limited amount of fermentation inhibitors such as acetic acid (<1.5 g/L), furfural (<0.1 g/L) and hydroxymethylfufural (HMF) (<0.1 g/L).
520 $a The bioconversion of wood hydrolysates to PHB was successfully performed using mixed microbial cultures (MMCs). Through the optimization of fed-batch experiments using a statistical design of experiments, several bioreactors could finally reach the steady states with reasonable PHB yields. The maximum PHB accumulated was 27% cell dry weight with a yield of 0.32 g/g (g PHB produced per g sugars consumed). Microbial community analysis was done at genus level, by next generation genomic sequencing, and the results showed that MMCs presented a community evolution in different bioreactors and during operational period. Several characterized genera belonged to Alphaproteobacteria and Betaproteobacteria classes such as Rhizobium, Sphingobium, Paracoccus, and Amaricoccus which have been characterized as PHA-storing microorganisms.
520 $a PHB Polymer characterization (chemical structure, PHB content modelling by FTIR, molecular weight, thermal and mechanical properties) had been evaluated for isolated PHB derived from MMC and compared with isolated pure culture PHB and a commercial PHB. Results showed that the properties of MMC PHB were similar to isolated pure culture and commercial PHB, which proved that MMC PHB was a good replacement for pure culture PHB.
590 $a School code: 0089.
650 4 $a Plastics Technology. $3 1023683
650 4 $a Chemistry, Polymer. $3 1018428
650 4 $a Agriculture, Wood Technology. $3 1031154
690 $a 0795
690 $a 0495
690 $a 0746
710 2 $a University of Idaho. $b Natural Resources. $3 3169373
773 0 $t Dissertation Abstracts International $g 75-11B(E).
790 $a 0089
791 $a Ph.D.
792 $a 2014
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3627719