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Low-cost methods for molecular chara...

Till, Bradley J.

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Low-cost methods for molecular characterization of mutant plants = tissue desiccation, DNA extraction and mutation discovery: protocols /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Low-cost methods for molecular characterization of mutant plants/ by Bradley J. Till ... [et al.].
Reminder of title:	tissue desiccation, DNA extraction and mutation discovery: protocols /
other author:	Till, Bradley J.
Published:	Cham :Springer International Publishing : : 2015.,
Description:	x, 35 p. :ill. (some col.), digital ;24 cm.
[NT 15003449]:	Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.
Contained By:	Springer eBooks
Subject:	Plant molecular genetics. -
Online resource:	http://dx.doi.org/10.1007/978-3-319-16259-1
ISBN:	9783319162591 (electronic bk.)

Low-cost methods for molecular characterization of mutant plants = tissue desiccation, DNA extraction and mutation discovery: protocols /
Low-cost methods for molecular characterization of mutant plantstissue desiccation, DNA extraction and mutation discovery: protocols /[electronic resource] :by Bradley J. Till ... [et al.]. - Cham :Springer International Publishing :2015. - x, 35 p. :ill. (some col.), digital ;24 cm.

Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

ISBN: 9783319162591 (electronic bk.)

Standard No.: 10.1007/978-3-319-16259-1doiSubjects--Topical Terms:

592577
Plant molecular genetics.

LC Class. No.: QK981.4

Dewey Class. No.: 572.82

Low-cost methods for molecular characterization of mutant plants = tissue desiccation, DNA extraction and mutation discovery: protocols /
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245 0 0 $a Low-cost methods for molecular characterization of mutant plants $h [electronic resource] : $b tissue desiccation, DNA extraction and mutation discovery: protocols / $c by Bradley J. Till ... [et al.].
260 $a Cham : $b Springer International Publishing : $b Imprint: Springer, $c 2015.
300 $a x, 35 p. : $b ill. (some col.), digital ; $c 24 cm.
505 0 $a Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.
520 $a This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
650 0 $a Plant molecular genetics. $3 592577
650 0 $a Plant hybridization $x Molecular aspects. $3 2152936
650 1 4 $a Life Sciences. $3 890838
650 2 4 $a Plant Breeding/Biotechnology. $3 899391
650 2 4 $a Biological Techniques. $3 900035
650 2 4 $a Nucleic Acid Chemistry. $3 1243992
700 1 $a Till, Bradley J. $3 2152935
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856 4 0 $u http://dx.doi.org/10.1007/978-3-319-16259-1
950 $a Biomedical and Life Sciences (Springer-11642)