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The effect of increased mitochondria...

Dillon, Lloye M.

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The effect of increased mitochondrial biogenesis and activation of PPAR pathways in a mouse model of aging.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	The effect of increased mitochondrial biogenesis and activation of PPAR pathways in a mouse model of aging./
Author:	Dillon, Lloye M.
Description:	139 p.
Notes:	Source: Dissertation Abstracts International, Volume: 74-02(E), Section: B.
Contained By:	Dissertation Abstracts International74-02B(E).
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3539081
ISBN:	9781267628183

The effect of increased mitochondrial biogenesis and activation of PPAR pathways in a mouse model of aging.
Dillon, Lloye M.

The effect of increased mitochondrial biogenesis and activation of PPAR pathways in a mouse model of aging. - 139 p.

Source: Dissertation Abstracts International, Volume: 74-02(E), Section: B.

Thesis (Ph.D.)--University of Miami, 2012.

Aging is the progressive decline in cellular, tissue and organ function. The mitochondrial theory of aging suggests that the accumulation of mitochondrial DNA (mtDNA) mutations leads to mitochondrial dysfunction, loss of organ function and consequently a decrease in lifespan. This theory is appealing as there is a correlation between age-dependent alterations in mtDNA and an increased risk for developing cardiovascular diseases, neurodegenerative disorders and myopathy. To further investigate the role of mtDNA mutations in aging, the mtDNA mutator mouse, a mouse model with a proof-reading deficient mtDNA polymerase gamma (POLG) was created. These mice have a premature aging phenotype and develop hair loss, anemia, kyphosis, sarcopenia, cardiomyopathy and decreased lifespan. This phenotype was associated with an accumulation of mtDNA mutations and mitochondrial dysfunction, suggesting that there is a link between mtDNA mutations, mitochondrial dysfunction and the aging phenotype in mammals.

ISBN: 9781267628183Subjects--Topical Terms:

1017686
Biology, Cell.

The effect of increased mitochondrial biogenesis and activation of PPAR pathways in a mouse model of aging.
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100 1 $a Dillon, Lloye M. $3 2105086
245 1 4 $a The effect of increased mitochondrial biogenesis and activation of PPAR pathways in a mouse model of aging.
300 $a 139 p.
500 $a Source: Dissertation Abstracts International, Volume: 74-02(E), Section: B.
500 $a Adviser: Carlos T. Moraes.
502 $a Thesis (Ph.D.)--University of Miami, 2012.
520 $a Aging is the progressive decline in cellular, tissue and organ function. The mitochondrial theory of aging suggests that the accumulation of mitochondrial DNA (mtDNA) mutations leads to mitochondrial dysfunction, loss of organ function and consequently a decrease in lifespan. This theory is appealing as there is a correlation between age-dependent alterations in mtDNA and an increased risk for developing cardiovascular diseases, neurodegenerative disorders and myopathy. To further investigate the role of mtDNA mutations in aging, the mtDNA mutator mouse, a mouse model with a proof-reading deficient mtDNA polymerase gamma (POLG) was created. These mice have a premature aging phenotype and develop hair loss, anemia, kyphosis, sarcopenia, cardiomyopathy and decreased lifespan. This phenotype was associated with an accumulation of mtDNA mutations and mitochondrial dysfunction, suggesting that there is a link between mtDNA mutations, mitochondrial dysfunction and the aging phenotype in mammals.
520 $a The work presented in this dissertation demonstrates three strategies employed to compensate for mitochondrial dysfunction in aging using the mutator mouse as a model system. We illustrate that increased mitochondrial biogenesis and activation of peroxisome proliferator-activated receptor (PPAR) pathways can improve some aging phenotypes in the mutator mouse. In chapter 2, we show that increased expression of PPAR gamma coactivator-1 alpha (PGC-1alpha), a crucial regulator of mitochondrial biogenesis and function, in muscle of mutator mice increased mitochondrial biogenesis and function, and also improved the skeletal muscle and heart phenotypes of the mice. However, deep sequencing analysis of mtDNA showed that the increased mitochondrial biogenesis did not reduce the accumulation of mtDNA mutations in the mutator mouse but rather caused a small increase. Therefore, our results indicate that increased muscle PGC-1alpha expression is able to improve some premature aging phenotypes in the mutator mice without reverting the accumulation of mtDNA mutations.
520 $a Bezafibrate is pharmacological agent that activates peroxisome proliferator-activated receptors (PPARs) and PGC-1alpha pathways that has been shown to improve mitochondrial function and energy metabolism. In chapter 3 we show that mutator mice treated with bezafibrate for 8-months had delayed hair loss and improved skin and spleen phenotypes. Bezafibrate did not induce global mitochondrial biogenesis/function in mutator mice; instead it increased mostly markers of fatty acid oxidation. Although we observed positive effects, bezafibrate induced hepatomegaly and did not slow the development of sarcopenia or increased the lifespan of the mutator mice. Our results show that despite its toxic effects, bezafibrate improved some aging phenotypes in the mutator mouse.
520 $a Because increased PGC-1alpha expression in muscle conferred benefits to mutator mice, in chapter 4 we created wild-type and mutator mice that inducibly and ubiquitously express either PGC-1alpha or its family member PGC-1beta. We found that increased systemic expression of PGC-1beta was toxic and caused lethality, however, ubiquitous induction of PGC-1alpha did not appear to be deleterious. These animals are valuable tools for studying the effects of systemic increases in mitochondrial biogenesis during aging.
590 $a School code: 0125.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Health Sciences, Aging. $3 1669845
690 $a 0379
690 $a 0307
690 $a 0493
710 2 $a University of Miami. $b Molecular Cell and Developmental Biology (Medicine). $3 2105087
773 0 $t Dissertation Abstracts International $g 74-02B(E).
790 $a 0125
791 $a Ph.D.
792 $a 2012
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3539081