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Structural analysis of carbohydrates...

Konda, Chiharu.

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Structural analysis of carbohydrates by mass spectrometry.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Structural analysis of carbohydrates by mass spectrometry./
作者:	Konda, Chiharu.
面頁冊數:	170 p.
附註:	Source: Dissertation Abstracts International, Volume: 75-06(E), Section: B.
Contained By:	Dissertation Abstracts International75-06B(E).
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3613165
ISBN:	9781303752933

Structural analysis of carbohydrates by mass spectrometry.
Konda, Chiharu.

Structural analysis of carbohydrates by mass spectrometry. - 170 p.

Source: Dissertation Abstracts International, Volume: 75-06(E), Section: B.

Thesis (Ph.D.)--Purdue University, 2013.

Protein glycosylation is a highly frequent post-translational modification. Glycosylation is actively involved in intermolecular and intercellular binding events that are important to a wide range of biological functions such as immunity and fertility. The unique functions of glycans are directly related to their structures. In order to fully characterize the structure of an unknown glycan, 5 levels of structural information is needed: sugar unit identity, anomeric configuration, linkage types, sequence, and branching location. One of the grand challenges limiting the advance of glycosciences is the lack of sensitive tools for detailed glycan structural analysis. Tandem mass spectrometry (MS/MS) is a powerful tool for structural analysis of carbohydrates with its high sensitivity and selectivity. MS2 (two-stage tandem mass spectrometry) is most often adopted due to the wide availability of commercial instruments; however, glycan structural determination typically stops at the level of topology (relative location of sugar unites). The goal of this research is to develop a multiple stage tandem mass spectrometry approach toward a sensitive and full level structural analysis of linear oligosaccharides by obtaining information of individual sugar unit identity, anomeric configuration, linkage types, and sequence. This approach roots in the discovery of diagnostic ions, which are sub-structures of disaccharides and the fragmentation patterns of which can be linked to a specific level of structural information. An MS 3 screening method was developed and a new diagnostic ion (Z 1) for linkage determination was established for the first time. With the use of another diagnostic ion (non-reducing sugar glycosidically linked to glycolaldehyde, m/z 221) discovered by Bendiak group, identity, anomeric information, and linkage position of the non-reducing sugar within a disaccharide can be determined from MS3 experiments. By utilizing pattern comparison algorithm, spectral similarity score, highly confident structural information can be obtained automatically. The strategy of diagnostic ions was further demonstrated on model linear oligosaccharides using MSn (n =3-5) for full level structural determination.

ISBN: 9781303752933Subjects--Topical Terms:

586156
Chemistry, Analytical.

Structural analysis of carbohydrates by mass spectrometry.
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