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Method development for a chemical an...

Lozano, Maria del Carmen.

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Method development for a chemical and biological analysis of glucocorticoids in wastewater.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Method development for a chemical and biological analysis of glucocorticoids in wastewater./
Author:	Lozano, Maria del Carmen.
Description:	75 p.
Notes:	Source: Masters Abstracts International, Volume: 52-05.
Contained By:	Masters Abstracts International52-05(E).
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1552264
ISBN:	9781303715181

Method development for a chemical and biological analysis of glucocorticoids in wastewater.
Lozano, Maria del Carmen.

Method development for a chemical and biological analysis of glucocorticoids in wastewater. - 75 p.

Source: Masters Abstracts International, Volume: 52-05.

Thesis (M.S.)--The University of Texas at El Paso, 2013.

Glucocorticoids are a class of steroid hormones that can either be produced synthetically or naturally by the adrenal glands. The synthetic glucocorticoids are highly prescribed in the United States for their anti-inflammatory and immunosuppressive properties to treat a variety of ailments and diseases; however, these have been implicated in a number of adverse human conditions such as obesity, metabolic syndrome, immune-suppression, delayed puberty, adrenal insufficiency and Cushing Syndrome, among others.

ISBN: 9781303715181Subjects--Topical Terms:

586156
Chemistry, Analytical.

Method development for a chemical and biological analysis of glucocorticoids in wastewater.
LDR:04355nam a2200337 4500 001 1967588
005 20141124080557.5
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020 $a 9781303715181
035 $a (MiAaPQ)AAI1552264
035 $a AAI1552264
040 $a MiAaPQ $c MiAaPQ
100 1 $a Lozano, Maria del Carmen. $3 2104627
245 1 0 $a Method development for a chemical and biological analysis of glucocorticoids in wastewater.
300 $a 75 p.
500 $a Source: Masters Abstracts International, Volume: 52-05.
500 $a Adviser: Wen-Yee Lee.
502 $a Thesis (M.S.)--The University of Texas at El Paso, 2013.
520 $a Glucocorticoids are a class of steroid hormones that can either be produced synthetically or naturally by the adrenal glands. The synthetic glucocorticoids are highly prescribed in the United States for their anti-inflammatory and immunosuppressive properties to treat a variety of ailments and diseases; however, these have been implicated in a number of adverse human conditions such as obesity, metabolic syndrome, immune-suppression, delayed puberty, adrenal insufficiency and Cushing Syndrome, among others.
520 $a Given that synthetic and natural estrogens have been previously found in wastewater influent and effluent for the Paso Del Norte Region, we hypothesized that glucocorticoids would be detected at significant concentrations. The objective of this research was to develop and optimize a chemical and biological method for the detection of glucocorticoids using solid phase extraction (SPE) followed by high performance liquid chromatography mass spectrometry (HPLC-MS)--in addition to a biological analysis using a 4-hour yeast assay.
520 $a Four glucocorticoids were included in this study and they are cortisone, hydrocortisone, prednisone and prednisolone. The average glucocorticoid concentrations in influent and effluent ranged from 4.62 (prednisolone/cortisone) to 15.56 (prednisone) ng/L, and from 3.43 (prednisolone/cortisone) to 12.57 (prednisone) ng/L, respectively. Prednisone had the highest levels among the four glucocorticoids in all wastewater samples potentially reflecting a high consumption of prednisone for asthma and allergy treatments in our region. For the influent samples, the finding showed that the levels of glucocorticoid in the morning hour samples were higher than that in samples collected from the rest of the day. As for the effluent samples, glucocorticoids are higher in the 12 pm samples. A 17.9 (+/-9.7) % estimated removal for the Northwest Wastewater Treatment Plant was calculated. To date, research has not identified a specific technology or wastewater treatment process that has the potential to completely remove glucocorticoids from wastewater effluent however anaerobic treatment is highly recommended.
520 $a Two previously hGR transfected yeast cells were tested for detecting glucocorticoids in wastewater: one with a higher promoter activity (MCY-212) in comparison to a lower promoter activity yeast cell (DSY-1345). The half maximal effective concentrations, EC50, for the glucocorticoids of interest were found to be in the &mgr;M range which is equivalent to high mg/L concentration. The results indicated that the biological analysis is not a suitable method for analyzing glucocorticoid activity in wastewater since the sensitivity of the assay was not sufficient for samples with concentrations of ng/L levels.
520 $a Even though this research was focused on the Paso Del Norte region, the presence of glucocorticoids in the environment is a worldwide problem. Semi-arid and arid regions represent 30% of the world's continental area. Limited water resources have the potential to be highly impacted by anthropogenic activities. It is important to target the removal of glucocorticoids and other environmental endocrine disruptors during wastewater treatment processes to avoid further dispersal of these contaminants of emerging concern to freshwater systems and protect the water resources for the safety of public health. (Abstract shortened by UMI.).
590 $a School code: 0459.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Biology, Endocrinology. $3 1682242
650 4 $a Engineering, Environmental. $3 783782
690 $a 0486
690 $a 0409
690 $a 0775
710 2 $a The University of Texas at El Paso. $b Geology. $3 1057878
773 0 $t Masters Abstracts International $g 52-05(E).
790 $a 0459
791 $a M.S.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1552264