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Quantitative analysis of aminophosph...

Liu, Sichang.

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Quantitative analysis of aminophospholipids by chemical derivatization and tandem mass spectrometry.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Quantitative analysis of aminophospholipids by chemical derivatization and tandem mass spectrometry./
Author:	Liu, Sichang.
Description:	120 p.
Notes:	Source: Masters Abstracts International, Volume: 49-05, page: 3189.
Contained By:	Masters Abstracts International49-05.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1493382
ISBN:	9781124655512

Quantitative analysis of aminophospholipids by chemical derivatization and tandem mass spectrometry.
Liu, Sichang.

Quantitative analysis of aminophospholipids by chemical derivatization and tandem mass spectrometry. - 120 p.

Source: Masters Abstracts International, Volume: 49-05, page: 3189.

Thesis (M.S.)--Michigan State University, 2010.

Aminophospholipid metabolism and cellular signaling are highly integrated processes that regulate various cellular functions. Abnormalities in lipid composition have established roles in human diseases, for example hepatocarcinogenesis---a kind of liver cancer. Thus, a comprehensive comparative analysis of changes in aminophospholipid profiles that occur between normal and diseased cells or tissues may enable the in-depth characterization of specific lipids that can serve as epigenetic factors and metabolomic biomarkers. In an effort to gain a precise and accurate relative quantification of aminophospholipids, one-step isotopic derivatization assay coupled with tandem mass spectrometry analysis was carried out. Heavy and light isotopic forms of a DMBNHS reagents which contain a positively charged sulfonium ion, were synthesized to efficiently and specifically derivatized amine-containing lipids. Aminophospholipids were subjected to such chemical derivatization in order to enhance ionization efficiency, assist structure elucidation and improved the precision of relative quantification by minimizing or negating errors associated with run-to-run irreproducibility. By mixing heavy- and light- labeled control and diseased mouse liver lipid extracts in 1 to 1 ratio's and performing a series of single-run neutral loss and precursor ion scan mode MS/MS experiments, we demonstrate significant alteration of aminophospholipid distributions of double mutant mice liver compared that of control mice.

ISBN: 9781124655512Subjects--Topical Terms:

586156
Chemistry, Analytical.

Quantitative analysis of aminophospholipids by chemical derivatization and tandem mass spectrometry.
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020 $a 9781124655512
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040 $a MiAaPQ $c MiAaPQ
100 1 $a Liu, Sichang. $3 2102746
245 1 0 $a Quantitative analysis of aminophospholipids by chemical derivatization and tandem mass spectrometry.
300 $a 120 p.
500 $a Source: Masters Abstracts International, Volume: 49-05, page: 3189.
500 $a Adviser: Gavin E. Reid.
502 $a Thesis (M.S.)--Michigan State University, 2010.
520 $a Aminophospholipid metabolism and cellular signaling are highly integrated processes that regulate various cellular functions. Abnormalities in lipid composition have established roles in human diseases, for example hepatocarcinogenesis---a kind of liver cancer. Thus, a comprehensive comparative analysis of changes in aminophospholipid profiles that occur between normal and diseased cells or tissues may enable the in-depth characterization of specific lipids that can serve as epigenetic factors and metabolomic biomarkers. In an effort to gain a precise and accurate relative quantification of aminophospholipids, one-step isotopic derivatization assay coupled with tandem mass spectrometry analysis was carried out. Heavy and light isotopic forms of a DMBNHS reagents which contain a positively charged sulfonium ion, were synthesized to efficiently and specifically derivatized amine-containing lipids. Aminophospholipids were subjected to such chemical derivatization in order to enhance ionization efficiency, assist structure elucidation and improved the precision of relative quantification by minimizing or negating errors associated with run-to-run irreproducibility. By mixing heavy- and light- labeled control and diseased mouse liver lipid extracts in 1 to 1 ratio's and performing a series of single-run neutral loss and precursor ion scan mode MS/MS experiments, we demonstrate significant alteration of aminophospholipid distributions of double mutant mice liver compared that of control mice.
590 $a School code: 0128.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Biology, Physiology. $3 1017816
690 $a 0486
690 $a 0487
690 $a 0719
710 2 $a Michigan State University. $3 676168
773 0 $t Masters Abstracts International $g 49-05.
790 $a 0128
791 $a M.S.
792 $a 2010
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1493382