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Recombinant expression of Lassa vira...

Zovanyi, Ashley M.

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Recombinant expression of Lassa viral proteins for diagnostic assays.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Recombinant expression of Lassa viral proteins for diagnostic assays./
Author:	Zovanyi, Ashley M.
Description:	116 p.
Notes:	Source: Masters Abstracts International, Volume: 49-02, page: 1094.
Contained By:	Masters Abstracts International49-02.
Subject:	Health Sciences, Immunology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1486970
ISBN:	9781124307442

Recombinant expression of Lassa viral proteins for diagnostic assays.
Zovanyi, Ashley M.

Recombinant expression of Lassa viral proteins for diagnostic assays. - 116 p.

Source: Masters Abstracts International, Volume: 49-02, page: 1094.

Thesis (M.S.)--Hood College, 2010.

Lassa virus is the cause of a hemorrhagic fever endemic to West Africa and Nigeria. Lassa virus affects 500,000 people annually and causes 5,000-10,000 deaths. With no vaccine and limited treatment for Lassa fever, rapid and accurate diagnosis is essential. Diagnostic methods which utilize molecular and immune techniques are preferred for early detection. Preparation of diagnostic reagents for Lassa virus requires the use of high level biocontainment which is expensive, dangerous and laborious. Recombinant proteins produced in a bacterial expression system provide a safer alternative to live or inactivated reagents.

ISBN: 9781124307442Subjects--Topical Terms:

1017716
Health Sciences, Immunology.

Recombinant expression of Lassa viral proteins for diagnostic assays.
LDR:01919nam a2200301 4500 001 1965976
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020 $a 9781124307442
035 $a (MiAaPQ)AAI1486970
035 $a AAI1486970
040 $a MiAaPQ $c MiAaPQ
100 1 $a Zovanyi, Ashley M. $3 2102740
245 1 0 $a Recombinant expression of Lassa viral proteins for diagnostic assays.
300 $a 116 p.
500 $a Source: Masters Abstracts International, Volume: 49-02, page: 1094.
500 $a Adviser: Janice Gilsdorf.
502 $a Thesis (M.S.)--Hood College, 2010.
520 $a Lassa virus is the cause of a hemorrhagic fever endemic to West Africa and Nigeria. Lassa virus affects 500,000 people annually and causes 5,000-10,000 deaths. With no vaccine and limited treatment for Lassa fever, rapid and accurate diagnosis is essential. Diagnostic methods which utilize molecular and immune techniques are preferred for early detection. Preparation of diagnostic reagents for Lassa virus requires the use of high level biocontainment which is expensive, dangerous and laborious. Recombinant proteins produced in a bacterial expression system provide a safer alternative to live or inactivated reagents.
520 $a Lassa nucleoprotein (NP) and glycoproteins (GP) 1 and 2 were expressed in E. coli and characterized with Western blot and Coomassie stained gels. The recombinant proteins were evaluated for antigenic authenticity and reactivity in enzyme-linked immunosorbent assays (ELISA). All three recombinant proteins were positive in direct ELISAs and the recombinant NP was successful in detecting anti-Lassa IgM and IgG antibodies.
590 $a School code: 0917.
650 4 $a Health Sciences, Immunology. $3 1017716
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, General. $3 1018625
690 $a 0982
690 $a 0307
690 $a 0306
710 2 $a Hood College. $3 1680845
773 0 $t Masters Abstracts International $g 49-02.
790 $a 0917
791 $a M.S.
792 $a 2010
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1486970