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Regulation of genome-wide transcript...

Cook, Kristen Elizabeth.

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Regulation of genome-wide transcriptional stress responses in Saccharomyces cerevisiae.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Regulation of genome-wide transcriptional stress responses in Saccharomyces cerevisiae./
Author:	Cook, Kristen Elizabeth.
Description:	129 p.
Notes:	Source: Dissertation Abstracts International, Volume: 73-06, Section: B, page: 3391.
Contained By:	Dissertation Abstracts International73-06B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3495606
ISBN:	9781267193384

Regulation of genome-wide transcriptional stress responses in Saccharomyces cerevisiae.
Cook, Kristen Elizabeth.

Regulation of genome-wide transcriptional stress responses in Saccharomyces cerevisiae. - 129 p.

Source: Dissertation Abstracts International, Volume: 73-06, Section: B, page: 3391.

Thesis (Ph.D.)--Harvard University, 2012.

In response to osmotic shock in Saccharomyces cerevisiae the MAP kinase Hog1 coordinates a large-scale transcriptional stress response, rapidly producing hundreds of copies of specified transcripts. Many of the most highly induced genes are bound and regulated by a transcription factor, Sko1, but lack the canonical binding site for this factor. We use ChIP-seq to demonstrate a stress-specific binding mode of Sko1. In stress, Sko1 binds to promoters in close proximity to Hog1, and another Hog1-regulated transcription factor, Hot1. This mode of Sko1 binding requires the physical presence of Hog1, but not Hog1 phosphorylation of Sko1. We identify candidate Sko1 and Hot1 binding motifs that predict co-localization of Sko1, Hot1, and Hog1 at promoters. We then demonstrate a role for Sko1 and Hot1 in directing Hog1-associated RNA Pol II to target genes, where Hog1 is present with the elongating polymerase. We suggest a possible model for Hog1 reprogramming of transcription in the early stages of the osmotic stress response. We then determine the extent and structure of the Hog1 controlled transcriptional program in a related stress, damage to the cell wall. We find that Sko1 and Hot1 have different apparent thresholds for activation by Hog1. In addition, in cell wall damage, Hog1 regulates an additional transcription factor, Rlm1, that is not involved in other Hog1 regulated stress responses. This factor is activated by the coincidence of a signal from Hog1 with that of another MAP kinase, Slt2.

ISBN: 9781267193384Subjects--Topical Terms:

1017734
Biology, Microbiology.

Regulation of genome-wide transcriptional stress responses in Saccharomyces cerevisiae.
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020 $a 9781267193384
035 $a (MiAaPQ)AAI3495606
035 $a AAI3495606
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100 1 $a Cook, Kristen Elizabeth. $3 2102382
245 1 0 $a Regulation of genome-wide transcriptional stress responses in Saccharomyces cerevisiae.
300 $a 129 p.
500 $a Source: Dissertation Abstracts International, Volume: 73-06, Section: B, page: 3391.
500 $a Includes supplementary digital materials.
500 $a Adviser: Erin K. O'Shea.
502 $a Thesis (Ph.D.)--Harvard University, 2012.
520 $a In response to osmotic shock in Saccharomyces cerevisiae the MAP kinase Hog1 coordinates a large-scale transcriptional stress response, rapidly producing hundreds of copies of specified transcripts. Many of the most highly induced genes are bound and regulated by a transcription factor, Sko1, but lack the canonical binding site for this factor. We use ChIP-seq to demonstrate a stress-specific binding mode of Sko1. In stress, Sko1 binds to promoters in close proximity to Hog1, and another Hog1-regulated transcription factor, Hot1. This mode of Sko1 binding requires the physical presence of Hog1, but not Hog1 phosphorylation of Sko1. We identify candidate Sko1 and Hot1 binding motifs that predict co-localization of Sko1, Hot1, and Hog1 at promoters. We then demonstrate a role for Sko1 and Hot1 in directing Hog1-associated RNA Pol II to target genes, where Hog1 is present with the elongating polymerase. We suggest a possible model for Hog1 reprogramming of transcription in the early stages of the osmotic stress response. We then determine the extent and structure of the Hog1 controlled transcriptional program in a related stress, damage to the cell wall. We find that Sko1 and Hot1 have different apparent thresholds for activation by Hog1. In addition, in cell wall damage, Hog1 regulates an additional transcription factor, Rlm1, that is not involved in other Hog1 regulated stress responses. This factor is activated by the coincidence of a signal from Hog1 with that of another MAP kinase, Slt2.
590 $a School code: 0084.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Systematic. $3 1676856
690 $a 0410
690 $a 0369
690 $a 0423
710 2 $a Harvard University. $b Biology, Molecular and Cellular. $3 2093132
773 0 $t Dissertation Abstracts International $g 73-06B.
790 $a 0084
791 $a Ph.D.
792 $a 2012
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3495606