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Biosensor development for environmen...

Liang, Pei-Shih.

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Biosensor development for environmental monitoring, food safety, and secondary education applications.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Biosensor development for environmental monitoring, food safety, and secondary education applications./
Author:	Liang, Pei-Shih.
Description:	116 p.
Notes:	Source: Dissertation Abstracts International, Volume: 75-04(E), Section: B.
Contained By:	Dissertation Abstracts International75-04B(E).
Subject:	Engineering, Agricultural. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3605082
ISBN:	9781303611124

Biosensor development for environmental monitoring, food safety, and secondary education applications.
Liang, Pei-Shih.

Biosensor development for environmental monitoring, food safety, and secondary education applications. - 116 p.

Source: Dissertation Abstracts International, Volume: 75-04(E), Section: B.

Thesis (Ph.D.)--The University of Arizona, 2013.

This dissertation develops biosensors for rapid detection of pathogens for environmental monitoring and food safety applications and utilizes the multidisciplinary and multi-application characteristics of biosensors to develop a lesson plan that can be implemented in secondary education classrooms. The detection methods evolve from particle immunoagglutination assay, PDMS optofluidic lab-on-a-chip, and spectrum analysis to smartphone and image analysis without any reagent; the potential application in secondary education also underlines the extended value of biosensors.

ISBN: 9781303611124Subjects--Topical Terms:

1019504
Engineering, Agricultural.

Biosensor development for environmental monitoring, food safety, and secondary education applications.
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020 $a 9781303611124
035 $a (MiAaPQ)AAI3605082
035 $a AAI3605082
040 $a MiAaPQ $c MiAaPQ
100 1 $a Liang, Pei-Shih. $3 2101298
245 1 0 $a Biosensor development for environmental monitoring, food safety, and secondary education applications.
300 $a 116 p.
500 $a Source: Dissertation Abstracts International, Volume: 75-04(E), Section: B.
500 $a Adviser: Jeong-Yeol Yoon.
502 $a Thesis (Ph.D.)--The University of Arizona, 2013.
520 $a This dissertation develops biosensors for rapid detection of pathogens for environmental monitoring and food safety applications and utilizes the multidisciplinary and multi-application characteristics of biosensors to develop a lesson plan that can be implemented in secondary education classrooms. The detection methods evolve from particle immunoagglutination assay, PDMS optofluidic lab-on-a-chip, and spectrum analysis to smartphone and image analysis without any reagent; the potential application in secondary education also underlines the extended value of biosensors.
520 $a In the first paper presented here, an optofluidic lab-on-a-chip system and subsequent sampling procedure were developed for detecting bacteria from soil samples utilizing Mie scattering detection of particle immunoagglutination assay. This system and protocol detected the presence of Escherichia coli K12 from soil particles in near real-time (10 min) with a detection limit down to 1 CFU mL-1 and has the potential to be implemented in the field. We also compared the interaction between E. coli and soil particles to the two-step protein-surface interaction.
520 $a In the second paper, a smartphone-utilized biosensor consisting of a near-infrared (NIR) LED (wavelength of 880 nm) and a digital camera of a smartphone was developed for detecting microbial spoilage on ground beef, without using any reagents. The method was further improved by programming a smartphone application that allows the user to position the smartphone at an optimum distance and a range of angles utilizing its internal gyro sensor to measure a series of scatter intensities against the detection angle. This handheld device can be used as a preliminary screening tool to monitor microbial contamination on meat products.
520 $a In the third paper, we designed a lesson plan for secondary education classrooms using biosensors as a core and branching out to different applications and fields of study with the goal of heightening students' interest and motivation toward attaining degrees and careers in STEM fields. Results revealed that the lesson was more effective in affecting younger students than older students, and more effective in teaching about the applications of biosensors than about the techniques of biosensor development.
590 $a School code: 0009.
650 4 $a Engineering, Agricultural. $3 1019504
650 4 $a Engineering, Environmental. $3 783782
650 4 $a Agriculture, Food Science and Technology. $3 1017813
690 $a 0539
690 $a 0775
690 $a 0359
710 2 $a The University of Arizona. $b Agricultural & Biosystems Engineering. $3 1019524
773 0 $t Dissertation Abstracts International $g 75-04B(E).
790 $a 0009
791 $a Ph.D.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3605082