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Ultrastable Atomic Force Microscopy ...

Churnside, Allison B.

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Ultrastable Atomic Force Microscopy for Biophysics.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Ultrastable Atomic Force Microscopy for Biophysics./
Author:	Churnside, Allison B.
Description:	177 p.
Notes:	Source: Dissertation Abstracts International, Volume: 74-09(E), Section: B.
Contained By:	Dissertation Abstracts International74-09B(E).
Subject:	Nanotechnology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3561940
ISBN:	9781303093524

Ultrastable Atomic Force Microscopy for Biophysics.
Churnside, Allison B.

Ultrastable Atomic Force Microscopy for Biophysics. - 177 p.

Source: Dissertation Abstracts International, Volume: 74-09(E), Section: B.

Thesis (Ph.D.)--University of Colorado at Boulder, 2013.

Atomic force microscopy (AFM) is a multifunctional workhorse of nanoscience and molecular biophysics, but instrumental drift remains a critical issue that limits the precision and duration of experiments. We have significantly reduced the two most important types of drift: in position and in force. The first, position drift, is defined as uncontrolled motion between the tip and the sample, which occurs in all three dimensions. By scattering a laser off the apex of a commercial AFM tip, we locally measured and thereby actively controlled its three-dimensional position above a sample surface to <0.4 A (Deltaf = 0.01--10 Hz) in air at room temperature. With this enhanced stability, we demonstrated atomic-scale (&sim;1 A) tip-sample stability and registration over tens of minutes with a series of AFM images. The second type of drift is force drift. We found that the primary source of force drift for a popular class of soft cantilevers is their gold coating, even though they are coated on both sides to minimize drift. When the gold coating was removed through a simple chemical etch, this drift in deflection was reduced by more than an order of magnitude over the first 2 hours after wetting the tip. Removing the gold also led to &sim; 10-fold reduction in reflected light, yet short-term (0.1--10 s) force precision improved. With both position and force drift greatly reduced, the utility of the AFM is enhanced. These improvements led to several new AFM abilities, including a five-fold increase in the image signal-to-noise ratio; tip-registered, label-free optical imaging; registered tip return to a particular point on the sample; and dual-detection force spectroscopy, which enables a new extension clamp mode. We have applied these abilities to folding of both membrane and soluble proteins. In principle, the techniques we describe can be fully incorporated into many types of scanning probe microscopy, making this work a general improvement to scanning probe techniques.

ISBN: 9781303093524Subjects--Topical Terms:

526235
Nanotechnology.

Ultrastable Atomic Force Microscopy for Biophysics.
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020 $a 9781303093524
035 $a (MiAaPQ)AAI3561940
035 $a AAI3561940
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100 1 $a Churnside, Allison B. $3 2101194
245 1 0 $a Ultrastable Atomic Force Microscopy for Biophysics.
300 $a 177 p.
500 $a Source: Dissertation Abstracts International, Volume: 74-09(E), Section: B.
500 $a Advisers: Thomas T. Perkins; Kyle McElroy.
502 $a Thesis (Ph.D.)--University of Colorado at Boulder, 2013.
520 $a Atomic force microscopy (AFM) is a multifunctional workhorse of nanoscience and molecular biophysics, but instrumental drift remains a critical issue that limits the precision and duration of experiments. We have significantly reduced the two most important types of drift: in position and in force. The first, position drift, is defined as uncontrolled motion between the tip and the sample, which occurs in all three dimensions. By scattering a laser off the apex of a commercial AFM tip, we locally measured and thereby actively controlled its three-dimensional position above a sample surface to <0.4 A (Deltaf = 0.01--10 Hz) in air at room temperature. With this enhanced stability, we demonstrated atomic-scale (&sim;1 A) tip-sample stability and registration over tens of minutes with a series of AFM images. The second type of drift is force drift. We found that the primary source of force drift for a popular class of soft cantilevers is their gold coating, even though they are coated on both sides to minimize drift. When the gold coating was removed through a simple chemical etch, this drift in deflection was reduced by more than an order of magnitude over the first 2 hours after wetting the tip. Removing the gold also led to &sim; 10-fold reduction in reflected light, yet short-term (0.1--10 s) force precision improved. With both position and force drift greatly reduced, the utility of the AFM is enhanced. These improvements led to several new AFM abilities, including a five-fold increase in the image signal-to-noise ratio; tip-registered, label-free optical imaging; registered tip return to a particular point on the sample; and dual-detection force spectroscopy, which enables a new extension clamp mode. We have applied these abilities to folding of both membrane and soluble proteins. In principle, the techniques we describe can be fully incorporated into many types of scanning probe microscopy, making this work a general improvement to scanning probe techniques.
590 $a School code: 0051.
650 4 $a Nanotechnology. $3 526235
650 4 $a Biophysics, General. $3 1019105
650 4 $a Nanoscience. $3 587832
690 $a 0652
690 $a 0786
690 $a 0565
710 2 $a University of Colorado at Boulder. $b Physics. $3 1019557
773 0 $t Dissertation Abstracts International $g 74-09B(E).
790 $a 0051
791 $a Ph.D.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3561940