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Mass Spectral Analysis of Crustacean...

The University of Wisconsin - Madison., Chemistry.

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Mass Spectral Analysis of Crustacean Signaling Peptides Using a Multi-dimensional Strategy.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Mass Spectral Analysis of Crustacean Signaling Peptides Using a Multi-dimensional Strategy./
Author:	Woodards, Nicole Adelita.
Description:	338 p.
Notes:	Source: Dissertation Abstracts International, Volume: 74-12(E), Section: B.
Contained By:	Dissertation Abstracts International74-12B(E).
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3592400
ISBN:	9781303334306

Mass Spectral Analysis of Crustacean Signaling Peptides Using a Multi-dimensional Strategy.
Woodards, Nicole Adelita.

Mass Spectral Analysis of Crustacean Signaling Peptides Using a Multi-dimensional Strategy. - 338 p.

Source: Dissertation Abstracts International, Volume: 74-12(E), Section: B.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2013.

By definition, neuropeptides are a class of signaling molecules either secreted by or that have an effect on neurons. They are present at minute concentrations in complex biological matrices. Their expression levels vary both temporally and spatially as they regulate several biological functions. Our limited knowledge of their regulatory processes has resulted in the creation or understanding of several therapeutic treatments with more advancements hinging on future neuropeptidomic breakthroughs.

ISBN: 9781303334306Subjects--Topical Terms:

586156
Chemistry, Analytical.

Mass Spectral Analysis of Crustacean Signaling Peptides Using a Multi-dimensional Strategy.
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100 1 $a Woodards, Nicole Adelita. $3 2100496
245 1 0 $a Mass Spectral Analysis of Crustacean Signaling Peptides Using a Multi-dimensional Strategy.
300 $a 338 p.
500 $a Source: Dissertation Abstracts International, Volume: 74-12(E), Section: B.
500 $a Adviser: Lingjun Li.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2013.
520 $a By definition, neuropeptides are a class of signaling molecules either secreted by or that have an effect on neurons. They are present at minute concentrations in complex biological matrices. Their expression levels vary both temporally and spatially as they regulate several biological functions. Our limited knowledge of their regulatory processes has resulted in the creation or understanding of several therapeutic treatments with more advancements hinging on future neuropeptidomic breakthroughs.
520 $a A multi-dimensional strategy has been implemented for the analysis of neuropeptides in the crustacean model system. Due to their dynamic nature, biological mass spectrometry is the instrumental platform used for neuropeptide analysis. It is coupled with analytical methodologies primarily focused on improving neuropeptide detection, identification, and quantitation.
520 $a Simple protocols for rapid, robust, and reproducible analysis have been developed by modifying previous techniques for novel application on newer technologies. Several protocols were developed by acknowledging both the benefits and limitations of the techniques implemented. While this work utilizes both soft ionization techniques, more emphasis is given to the development of MALDI analysis.
520 $a Relative quantitative analysis by 4-plex N,N-dimethyl leucine (DiLeu) labels was applied to study the neuropeptide expression level changes in relation to feeding. A neuropeptide database was compiled for automated analysis of large datasets using popular protein identification and quantitation software.
520 $a Improving mass spectrometry de novo sequencing was achieved by modifying a simple acid-driven deamidation protocol to determine the presence and location of amides. Also, the formation of multiply charged peptides by MALDI ionization was explored to improve fragmentation spectra.
520 $a Finally, to further analysis by MALDI ionization, proof-of-principle projects were initiated. The first was established to improve detection of hemolymph samples on newer MALDI instruments to determine expression level changes of circulating neuropeptides in relation to the circadian rhythm cycle. The second was the application of isotopic DiLeu (iDiLeu) labels to generate a five point standard curve for absolute quantitation. Primary interests of these labels are in their application for imaging experiments. In conclusion, all projects were developed by implementing a multi-dimensional strategy to strengthen the mass spectrometric platform for advancing neuropeptidomic research.
590 $a School code: 0262.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0486
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710 2 $a The University of Wisconsin - Madison. $b Chemistry. $3 2049906
773 0 $t Dissertation Abstracts International $g 74-12B(E).
790 $a 0262
791 $a Ph.D.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3592400