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Fast quantitative analysis of metabo...

Yang, Qian.

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Fast quantitative analysis of metabolic biomarkers for clinical screening using mass spectrometry.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Fast quantitative analysis of metabolic biomarkers for clinical screening using mass spectrometry./
作者:	Yang, Qian.
面頁冊數:	122 p.
附註:	Source: Dissertation Abstracts International, Volume: 74-12(E), Section: B.
Contained By:	Dissertation Abstracts International74-12B(E).
標題:	Engineering, Biomedical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3592157
ISBN:	9781303330247

Fast quantitative analysis of metabolic biomarkers for clinical screening using mass spectrometry.
Yang, Qian.

Fast quantitative analysis of metabolic biomarkers for clinical screening using mass spectrometry. - 122 p.

Source: Dissertation Abstracts International, Volume: 74-12(E), Section: B.

Thesis (Ph.D.)--Purdue University, 2013.

The utilization of mass spectrometry (MS) in clinical biomarker screening is undergoing considerable expansion. MS is powerful in identifying different compounds in biological samples with high specificity and high sensitivity in a high-throughput manner. Sampling and ionization is the most diverse and key process that affect the efficiency, sensitivity and practicability for analyzing biological samples using mass spectrometry.

ISBN: 9781303330247Subjects--Topical Terms:

1017684
Engineering, Biomedical.

Fast quantitative analysis of metabolic biomarkers for clinical screening using mass spectrometry.
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245 1 0 $a Fast quantitative analysis of metabolic biomarkers for clinical screening using mass spectrometry.
300 $a 122 p.
500 $a Source: Dissertation Abstracts International, Volume: 74-12(E), Section: B.
500 $a Adviser: Zheng Ouyang.
502 $a Thesis (Ph.D.)--Purdue University, 2013.
520 $a The utilization of mass spectrometry (MS) in clinical biomarker screening is undergoing considerable expansion. MS is powerful in identifying different compounds in biological samples with high specificity and high sensitivity in a high-throughput manner. Sampling and ionization is the most diverse and key process that affect the efficiency, sensitivity and practicability for analyzing biological samples using mass spectrometry.
520 $a Collection of blood samples using cellulose-based paper card is a standard and effective process in neonatal screening and is being explored for therapeutic drug analysis. In comparison with liquid blood sample, dried blood spots (DBS) on paper cards are convenient for transportation and analytes are shown to be more stable for a longer storage. In addition, a much smaller volume of blood is sufficient for several replicate analyses; this is of significant advantage for infants and critically ill patients as blood collected by finger pricks or heel pricks, instead of venipuncture, can fulfill the requirement. The analysis of DBS on paper cards using MS still require a complex multi-step procedure of sample treatment, involving punching a paper disk containing the samples, extracting samples using solvent, purifying the analytes, and chromatography separation.
520 $a Paper spray as an integrated sampling and ionization technique was invented recently in our group. Paper spray generates ions directly from the samples on the paper substrate by applying solvent and high voltage, which serves as a simple way for direct clinical biomarker screening using MS without the need for complex sample preparation and separation. This single step technique can be applied for quantitatively analyzing multiple compounds and is expected to become a fast, simple, effective and in-expensive route for clinical biomarker screening. The research and development includes a series of steps, including a systematical study of the paper spray ionization mechanism, design of disposable sample cartridge, selection of the proper biomarkers, and improvement of the method for quantitative analysis. The methodology developed is to be applied clinical screening of biomarkers for newborn screening. Finally, a proof-of-concept prototype of high-throughput paper spray device was built for direct and fast analysis of raw biological samples.
590 $a School code: 0183.
650 4 $a Engineering, Biomedical. $3 1017684
650 4 $a Chemistry, Analytical. $3 586156
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710 2 $a Purdue University. $b Biomedical Engineering. $3 1019122
773 0 $t Dissertation Abstracts International $g 74-12B(E).
790 $a 0183
791 $a Ph.D.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3592157