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Microfluidic platforms for one- and ...

Donoghue, Margaret A.

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Microfluidic platforms for one- and two-dimensional protein separations.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Microfluidic platforms for one- and two-dimensional protein separations./
作者:	Donoghue, Margaret A.
面頁冊數:	179 p.
附註:	Source: Dissertation Abstracts International, Volume: 71-07, Section: B, page: 4219.
Contained By:	Dissertation Abstracts International71-07B.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3409097
ISBN:	9781124061085

Microfluidic platforms for one- and two-dimensional protein separations.
Donoghue, Margaret A.

Microfluidic platforms for one- and two-dimensional protein separations. - 179 p.

Source: Dissertation Abstracts International, Volume: 71-07, Section: B, page: 4219.

Thesis (Ph.D.)--Indiana University, 2010.

Two-dimensional separations provide the high peak capacities necessary to analyze complex biological samples, and advantages of microfluidic separations include the ability to handle small samples on short time scales. We have been working towards developing microfluidic devices for two-dimensional separations with the goal of achieving the high peak capacities required for a wide variety of biological samples. The devices have a planar format, where the first dimension of a separation runs along one axis, and the second dimension is conducted orthogonally. Critical to device function is the ability to transfer sample efficiently from the first dimension to the second dimension. Initial efforts focused on device designs that would minimize band broadening upon the transfer of sample between dimensions. Several device variations were tested and optimized. Flow from four control channels adjacent to the first dimension and waste channels was used to confine sample into a narrow band within the open transfer region. Additionally, the incorporation of an array of parallel channels as a second dimension improved sample confinement. The narrowest sample streams were generated when a single control channel was combined with the close proximity of the parallel channel array. Sample handling was demonstrated on these chips, and sample could be routed into both the first and second dimensions easily. After the microfluidic device had been fully designed, capillary electrophoresis and capillary gel electrophoresis (CGE) were chosen as the separation methods to pursue. Prior to incorporation in the planar device, separations were optimized independently in single-channel devices. In this work, a standard molecular weight ladder and the soluble protein fraction of Caulobacter crescentus were analyzed on 10-cm long serpentine channels. These separations produced some of the highest plate numbers obtained for microchip CGE and were the longest analysis lengths to be used. Future modifications to the chip design will allow for the incorporation a wider variety of separation techniques, such as electrochromatography with gradient elution, and electrospray deposition onto plates for analysis by mass spectrometry with matrix-assisted laser desorption ionization.

ISBN: 9781124061085Subjects--Topical Terms:

586156
Chemistry, Analytical.

Microfluidic platforms for one- and two-dimensional protein separations.
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