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Biocontrol of foodborne bacterial pa...

Anany, Hany El-Said Mohamad.

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Biocontrol of foodborne bacterial pathogens using immobilized bacteriophages.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Biocontrol of foodborne bacterial pathogens using immobilized bacteriophages./
Author:	Anany, Hany El-Said Mohamad.
Description:	284 p.
Notes:	Source: Dissertation Abstracts International, Volume: 72-01, Section: B, page: 8000.
Contained By:	Dissertation Abstracts International72-01B.
Subject:	Agriculture, Food Science and Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NR67847
ISBN:	9780494678473

Biocontrol of foodborne bacterial pathogens using immobilized bacteriophages.
Anany, Hany El-Said Mohamad.

Biocontrol of foodborne bacterial pathogens using immobilized bacteriophages. - 284 p.

Source: Dissertation Abstracts International, Volume: 72-01, Section: B, page: 8000.

Thesis (Ph.D.)--University of Guelph (Canada), 2010.

The goal of the present research was to develop a simple technique to immobilize isolated lytic phages and explore the potential use of these immobilized phages to control certain foodborne pathogens in a real food system. More than one hundred phages were isolated from different environmental samples against different strains of four major foodborne pathogens; E. coli O157:H7, Salmonella, Listeria monocytogenes and Shigella. A turbidimetric method in high throughput format using the Bioscreen C was used to monitor phage lytic activity and determine the host range of the isolated phages. This enabled identification of isolated phages with similar characteristics and the selection of twelve good phage candidates for biocontrol purposes. These phages were characterized by TEM, restriction endonuclease pattern, one-step growth curve, BIM (bacteriphage insensitve mutant) development, cross infectivity and determination of their stability and infectivity under different conditions. A novel Shigella phage, &PHgr;SboM-AG3, was isolated and its genome sequenced. Its genome did not show any homology to any reported virulent or lysogenic genes and it was considered as a member of the "T4 superfamily". Phage cocktails made from the isolated phages were very effective for specific control of the four target pathogens in both broth media and contaminated food stored under different environmental conditions. The phages were immobilized using positively charged carrier substrates, which allowed specific 'binding of phages through their heads and leaving tail fibers free to interact with bacteria. These immobilized phages retained infectivity and immobilized Listeria and E. coli phage cocktails were able to control the growth of L. monocytogenes and E. coli O157:H7, respectively, in food under different temperatures and packaging conditions.

ISBN: 9780494678473Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

Biocontrol of foodborne bacterial pathogens using immobilized bacteriophages.
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100 1 $a Anany, Hany El-Said Mohamad. $3 2096839
245 1 0 $a Biocontrol of foodborne bacterial pathogens using immobilized bacteriophages.
300 $a 284 p.
500 $a Source: Dissertation Abstracts International, Volume: 72-01, Section: B, page: 8000.
502 $a Thesis (Ph.D.)--University of Guelph (Canada), 2010.
520 $a The goal of the present research was to develop a simple technique to immobilize isolated lytic phages and explore the potential use of these immobilized phages to control certain foodborne pathogens in a real food system. More than one hundred phages were isolated from different environmental samples against different strains of four major foodborne pathogens; E. coli O157:H7, Salmonella, Listeria monocytogenes and Shigella. A turbidimetric method in high throughput format using the Bioscreen C was used to monitor phage lytic activity and determine the host range of the isolated phages. This enabled identification of isolated phages with similar characteristics and the selection of twelve good phage candidates for biocontrol purposes. These phages were characterized by TEM, restriction endonuclease pattern, one-step growth curve, BIM (bacteriphage insensitve mutant) development, cross infectivity and determination of their stability and infectivity under different conditions. A novel Shigella phage, &PHgr;SboM-AG3, was isolated and its genome sequenced. Its genome did not show any homology to any reported virulent or lysogenic genes and it was considered as a member of the "T4 superfamily". Phage cocktails made from the isolated phages were very effective for specific control of the four target pathogens in both broth media and contaminated food stored under different environmental conditions. The phages were immobilized using positively charged carrier substrates, which allowed specific 'binding of phages through their heads and leaving tail fibers free to interact with bacteria. These immobilized phages retained infectivity and immobilized Listeria and E. coli phage cocktails were able to control the growth of L. monocytogenes and E. coli O157:H7, respectively, in food under different temperatures and packaging conditions.
590 $a School code: 0081.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0359
690 $a 0410
710 2 $a University of Guelph (Canada). $3 1018650
773 0 $t Dissertation Abstracts International $g 72-01B.
790 $a 0081
791 $a Ph.D.
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793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NR67847