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Developing white ash with resistance...

Palla, Kaitlin J.

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Developing white ash with resistance to the emerald ash borer.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Developing white ash with resistance to the emerald ash borer./
Author:	Palla, Kaitlin J.
Description:	124 p.
Notes:	Source: Masters Abstracts International, Volume: 52-03.
Contained By:	Masters Abstracts International52-03(E).
Subject:	Agriculture, Forestry and Wildlife. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1546993
ISBN:	9781303477003

Developing white ash with resistance to the emerald ash borer.
Palla, Kaitlin J.

Developing white ash with resistance to the emerald ash borer. - 124 p.

Source: Masters Abstracts International, Volume: 52-03.

Thesis (M.S.)--Purdue University, 2013.

Hypocotyls isolated from mature embryos germinated on Murashige and Skoog (MS) medium supplemented with 22.2 microM 6-benzyladenine (BA) and 0.5 microM thidiazuron (TDZ) were transformed using Agrobacterium tumefaciens strain EHA105 harboring the pBI121-8D2 vector containing the neomycin phosphotransferase (nptII) gene and a full length, codon-optimized Bacillus thuringiensis Cry8Da protoxin gene (cry8D2). Explants were transformed in a bacterial suspension with 100 microM acetosyringone using 90 s sonication and 10 min vacuum-infiltration. Four independent lines of transformed shoots were selectively regenerated on MS medium with 22.2 microM BA, 0.5 microM TDZ, 50 mg L-1 adenine sulfate, 10% coconut water, 30 mg L-1 kanamycin, and 500 mg L-1 timentin. The presence of cry8D2 and nptII were confirmed by polymerase chain reaction for all four transgenic lines. Transgenic white ash microshoots are being multiplied for use in future feeding trials to determine potential efficacy of the cry8D2 gene against emerald ash borer attack.

ISBN: 9781303477003Subjects--Topical Terms:

783690
Agriculture, Forestry and Wildlife.

Developing white ash with resistance to the emerald ash borer.
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020 $a 9781303477003
035 $a (MiAaPQ)AAI1546993
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245 1 0 $a Developing white ash with resistance to the emerald ash borer.
300 $a 124 p.
500 $a Source: Masters Abstracts International, Volume: 52-03.
500 $a Adviser: Paula M. Pijut.
502 $a Thesis (M.S.)--Purdue University, 2013.
520 $a Hypocotyls isolated from mature embryos germinated on Murashige and Skoog (MS) medium supplemented with 22.2 microM 6-benzyladenine (BA) and 0.5 microM thidiazuron (TDZ) were transformed using Agrobacterium tumefaciens strain EHA105 harboring the pBI121-8D2 vector containing the neomycin phosphotransferase (nptII) gene and a full length, codon-optimized Bacillus thuringiensis Cry8Da protoxin gene (cry8D2). Explants were transformed in a bacterial suspension with 100 microM acetosyringone using 90 s sonication and 10 min vacuum-infiltration. Four independent lines of transformed shoots were selectively regenerated on MS medium with 22.2 microM BA, 0.5 microM TDZ, 50 mg L-1 adenine sulfate, 10% coconut water, 30 mg L-1 kanamycin, and 500 mg L-1 timentin. The presence of cry8D2 and nptII were confirmed by polymerase chain reaction for all four transgenic lines. Transgenic white ash microshoots are being multiplied for use in future feeding trials to determine potential efficacy of the cry8D2 gene against emerald ash borer attack.
590 $a School code: 0183.
650 4 $a Agriculture, Forestry and Wildlife. $3 783690
650 4 $a Biology, Botany. $3 1017825
650 4 $a Agriculture, Plant Culture. $3 1018669
650 4 $a Natural Resource Management. $3 676989
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690 $a 0309
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710 2 $a Purdue University. $b Forestry and Natural Resources. $3 2093037
773 0 $t Masters Abstracts International $g 52-03(E).
790 $a 0183
791 $a M.S.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1546993