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Mass spectrometry of glycoproteins :...

Kohler, Jennifer, (1972-)

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Mass spectrometry of glycoproteins : = methods and protocols /

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Mass spectrometry of glycoproteins :/ edited by Jennifer J. Kohler, Steven M. Patrie.
其他題名:	methods and protocols /
其他作者:	Kohler, Jennifer,
出版者:	New York :Humana Press, : 2013.,
面頁冊數:	xi, 339 p. :ill. ;27 cm.
標題:	Glycoproteins - Spectra. -
ISBN:	9781627031455 (hbk.) :
ISSN:	10643745

Mass spectrometry of glycoproteins : = methods and protocols /
Mass spectrometry of glycoproteins :methods and protocols /edited by Jennifer J. Kohler, Steven M. Patrie. - New York :Humana Press,2013. - xi, 339 p. :ill. ;27 cm. - Methods in molecular biology,9511064-3745 ;. - Methods in molecular biology (Clifton, N.J.) ;v. 654..

Includes bibliographical references and index.

Introduction to glycosylation and mass spectrometry -- Enrichment and isolation methods --

This comprehensive new resource in the 'Methods in Molecular Biology' series features contributions from leading researchers who provide expert advice and reproducible, cutting-edge protocols for examining glycoproteins through mass spectrometry.

ISBN: 9781627031455 (hbk.) :EUR109.95

ISSN: 10643745

LCCN: 2012950354Subjects--Topical Terms:

2070249
Glycoproteins
--Spectra.

LC Class. No.: QP552.G59 / M37 2013

Dewey Class. No.: 572.6/8

Mass spectrometry of glycoproteins : = methods and protocols /
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504 $a Includes bibliographical references and index.
505 0 # $t Introduction to glycosylation and mass spectrometry -- $g Enrichment and isolation methods -- $t Tandem lectin weak affinity chromatography for glycoprotein enrichment -- $t CSC technology: Selective labeling of glycoproteins by mild oxidation to phenotype cells -- $t Use of boronic acid nanoparticles in glycoprotein enrichment -- $t Incorporation of unnatural sugars for the identification of glycoproteins -- $t Characterization of membrane-associated glycoproteins using lectin affinity chromatography and mass spectrometry -- $t Sialic acid capture-and-release and LC-MS(n) analysis of glycopeptides -- $g Sample preparation -- $t In-solution digestion of glycoproteins for glycopeptide-based mass analysis -- $t Nano-HPLC-MS of glycopeptides obtained after nonspecific proteolysis -- $g Separation methods -- $t Glycopeptide enrichment for MALDI-TOF mass spectrometry analysis by hydrophilic interaction liquid chromatography solid phase extraction (HILIC SPE) -- $t Separation and identification of glycoforms by capillary electrophoresis with electrospray ionization mass spectrometric detection -- $t Structural separations by ion mobility-MS for glycomics and glycoproteomics -- $g Quantitation methods -- $t Quantitative analysis of glycoprotein glycans -- $t Stable isotope labeling of N-glycosylated peptides by enzymatic deglycosylation for mass spectrometry-based glycoproteomics -- $t Approaches for site mapping and quantification of o-linked glycopeptides -- $t Glycan profiling : Label-free analysis of glycoproteins -- $g Computational tools -- $t Introduction to informatics in glycoprotein analysis -- $t Software tools for glycan profiling -- $g Case studies in mass spectrometary of Glycoproteins -- $t Quantitative characterization of glycoproteins in neurodegenerative disorders using iTRAQ -- $t Quantitative proteomic analysis of N-linked glycoproteins in human tear fluid -- $t Elucidation of N-glycosites within human plasma glycoproteins for cancer biomarker discovery -- $t Characterizing the glycosylation state of therapeutic recombinant glycoproteins.
520 8 $a This comprehensive new resource in the 'Methods in Molecular Biology' series features contributions from leading researchers who provide expert advice and reproducible, cutting-edge protocols for examining glycoproteins through mass spectrometry.
650 # 0 $a Glycoproteins $x Spectra. $3 2070249
650 # 0 $a Mass spectrometry. $3 551172
650 1 2 $a Glycoproteins $x chemistry. $3 2070250
650 2 2 $a Mass Spectrometry $x methods. $3 931459
700 1 # $a Kohler, Jennifer, $d 1972- $3 2070246
700 1 # $a Patrie, Steven M. $3 2070247
830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 654. $x 1064-3745 $3 1280414
830 0 $a Springer protocols (Series) $x 1949-2448 $3 2070248