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RNA methodologies (RDA 研究方法) : = lab...

Farrell, Robert E.

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RNA methodologies (RDA 研究方法) : = laboratory guide for isolation and characterization /

Record Type:	Language materials, printed : Monograph/item
Title/Author:	RNA methodologies (RDA 研究方法) :/ Robert E. Farrell Jr. ; 劉定干導讀.
Reminder of title:	laboratory guide for isolation and characterization /
Author:	Farrell, Robert E.
Published:	北京 :科學出版社, : 2011.,
Description:	xxiii, 717 p. :ill ;
Notes:	Previous ed.: 2005.
Subject:	RNA - Laboratory Manuals. - isolation & purification -
ISBN:	9787030306234 (hbk.) :

RNA methodologies (RDA 研究方法) : = laboratory guide for isolation and characterization /
Farrell, Robert E.

RNA methodologies (RDA 研究方法) :laboratory guide for isolation and characterization /Robert E. Farrell Jr. ; 劉定干導讀. - 4th ed. - 北京 :科學出版社,2011. - xxiii, 717 p. :ill ; - 實驗室解決方案.

Previous ed.: 2005.

Includes bibliographical references and index.

重溫RNA和細胞生物化學(RNA and the cellular biochemistry revisited) --

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary.

ISBN: 9787030306234 (hbk.) :RMB168.00

LCCN: 2005041012Subjects--Topical Terms:

1362168
RNA
--isolation & purification--Laboratory Manuals.

LC Class. No.: QP623 / .F37 2010

Dewey Class. No.: 572.88

RNA methodologies (RDA 研究方法) : = laboratory guide for isolation and characterization /
LDR:03874cam a22003374a 4500 001 1893134
003 OCoLC
005 20131114205038.0
008 131115s2010 ne a b 001 0 eng
010 $a 2005041012
020 $a 9787030306234 (hbk.) : $c RMB168.00
020 $a 7030306236 (hbk.)
020 $a 9780123747273 (hbk.)
020 $a 0123747279 (hbk.)
035 $a LIB-BE-100-035
040 $a NLM $b eng
050 0 4 $a QP623 $b .F37 2010
082 0 4 $a 572.88 $2 22
100 1 $a Farrell, Robert E. $3 1010557
245 1 0 $a RNA methodologies (RDA 研究方法) : $b laboratory guide for isolation and characterization / $c Robert E. Farrell Jr. ; 劉定干導讀.
250 $a 4th ed.
260 # $a 北京 : $b 科學出版社, $c 2011.
300 $a xxiii, 717 p. : $b ill ; $2 6 cm.
490 0 2 $a 實驗室解決方案
490 1 0 $a 科愛傳播導讀版
500 $a Previous ed.: 2005.
500 $a Elsevier原版引選 ; 導讀及目錄 chiefly 為中文簡體字.
504 $a Includes bibliographical references and index.
505 0 # $t 重溫RNA和細胞生物化學(RNA and the cellular biochemistry revisited) -- $t 分離RNA的策略(RNA isolation strategies) -- $t 關於組織的真相(The truth about tissues) -- $t 走向綠色:植物RNA及其分子生物學(Going green : RNA and the molecular biology of plants) -- $t 帶Poly A尾的RNA的分離(Isolation of polyadenylated RNA) -- $t RNA製品的質量控制(Quality control for RNA preparations) -- $t 棘手的核糖核酸酶(Resilient ribonucleases) -- $t 嚴謹度:影响核酸結構的條件(Stringency : conditions that influence nucleic acid structure) -- $t RNA電泳(Electrophoresis of RNA) -- $t 照相記錄和影像分析(Photodocumentation and image analysis) -- $t Northern分析(Northern analysis) -- $t 核酸探針技術(Nucleic acid probe technology) -- $t 核酸杂交的實踐(Practical nucleic acid hybridization) -- $t 檢出的原理(Principles of detection) -- $t 用抗核酸酶技定量特定的RNA(Quantifications of specific mRNAs by nuclease protection) -- $t 核RNA的分析(Analysis of nuclear RNA) -- $t cDNA合成(cDNA synthesis) -- $t 逆轉錄PCR:一種科學和技術形式(RT-PCR : a science and an art form) -- $t 定量PCR技術(Quantitative PCR techniques) -- $t 功能基因組學和轉錄物總體分析(Functional genomics and transcript profiling) -- $t 基因表速的高通量分析(High-troughput analysis of gene expression) -- $t 整體分析基因表速的非陣列方法(Non-array methods for global analysis of gene expression) -- $t RNA干擾:Dicer酶在RISC複合物起作用(RNAi : take a RISC-role the dicer) -- $t 基因組,轉錄組,蛋白質組和生物信息學(Genomes, transcriptomes, proteomes, and bioinformatics) -- $t RNA一例(An RNA paradigm).
520 # $a This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary.
650 1 2 $a RNA $x isolation & purification $v Laboratory Manuals. $3 1362168
650 2 2 $a Molecular Biology $x methods $v Laboratory Manuals. $3 1362169
650 2 2 $a RNA $x physiology $v Laboratory Manuals. $3 1362170
650 # 0 $a RNA $x Analysis $v Laboratory manuals. $3 1362171
740 0 2 $a RDA 研究方法
740 0 2 $a RDA 研究方法 = RDA methodologies
740 0 2 $a RDA 研究方法(實驗室解決方案)