東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Study of model lung surfactant: Pro...

Vickery, Sarah Ann.

FindBook

Google Book

Amazon

博客來

Study of model lung surfactant: Probing membrane structure from the macroscopic to the single molecule level.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Study of model lung surfactant: Probing membrane structure from the macroscopic to the single molecule level./
作者:	Vickery, Sarah Ann.
面頁冊數:	170 p.
附註:	Source: Dissertation Abstracts International, Volume: 63-11, Section: B, page: 5212.
Contained By:	Dissertation Abstracts International63-11B.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3071142
ISBN:	0493907750

Study of model lung surfactant: Probing membrane structure from the macroscopic to the single molecule level.
Vickery, Sarah Ann.

Study of model lung surfactant: Probing membrane structure from the macroscopic to the single molecule level. - 170 p.

Source: Dissertation Abstracts International, Volume: 63-11, Section: B, page: 5212.

Thesis (Ph.D.)--University of Kansas, 2002.

The high resolution techniques of confocal microscopy, atomic force microscopy (AFM), and near-field scanning optical microscopy (NSOM) are utilized to probe the structure of model lung surfactant (LS) from the macroscopic scale down to the single molecule level. Research has highlighted the particular importance of a surfactant-associated protein, SP-B, in stabilizing the pulmonary surfactant monolayer during respiration through a mechanism not fully understood. The aforementioned high resolution techniques are used, therefore, to provide insights into this mechanism through the study of Langmuir-Blodgett (LB) films of relevant systems. Background studies on palmitic acid (PA) are presented which provide insight into the phase behavior of the fatty acid and to elucidate factors contributing to the final film structure of supported two-dimensional films. These studies are then extended to model systems containing both PA and a truncated form of natural SP-B (SP-B1--25). Confocal microscopy studies are conducted on these films to provide a macroscopic scale picture of peptide concentration dependence. Evidence is presented indicating a bimodal dependence in the phase behavior of the films with peptide concentration. Further, the results suggest the addition of 11 wt.% SP-B1--25 to monolayers of PA induces large density fluctuations in the membrane over macroscopic length scales, a signature of critical behavior. These experiments on the macroscopic scale are extended to the single molecule level by exploiting the unique nature of the electric fields present at the NSOM tip aperture. Single molecule fluorescence NSOM measurements reveal the orientation distribution of single fluorescent probe molecules doped into LB films of DPPC. The data indicate that the environment of the probe molecules becomes more ordered as surface pressure is increased, as would be expected for lipid films of DPPC. Finally, these measurements will be further supplemented through a new technique presented here which combines attributes from NSOM and fluorescence resonance energy transfer (FRET) to increase optical imaging capabilities in both the lateral and axial directions. (Abstract shortened by UMI.)

ISBN: 0493907750Subjects--Topical Terms:

586156
Chemistry, Analytical.

Study of model lung surfactant: Probing membrane structure from the macroscopic to the single molecule level.
LDR:03167nmm 2200301 4500 001 1865179
005 20041216142220.5
008 130614s2002 eng d
020 $a 0493907750
035 $a (UnM)AAI3071142
035 $a AAI3071142
040 $a UnM $c UnM
100 1 $a Vickery, Sarah Ann. $3 1952638
245 1 0 $a Study of model lung surfactant: Probing membrane structure from the macroscopic to the single molecule level.
300 $a 170 p.
500 $a Source: Dissertation Abstracts International, Volume: 63-11, Section: B, page: 5212.
500 $a Adviser: Robert Dunn.
502 $a Thesis (Ph.D.)--University of Kansas, 2002.
520 $a The high resolution techniques of confocal microscopy, atomic force microscopy (AFM), and near-field scanning optical microscopy (NSOM) are utilized to probe the structure of model lung surfactant (LS) from the macroscopic scale down to the single molecule level. Research has highlighted the particular importance of a surfactant-associated protein, SP-B, in stabilizing the pulmonary surfactant monolayer during respiration through a mechanism not fully understood. The aforementioned high resolution techniques are used, therefore, to provide insights into this mechanism through the study of Langmuir-Blodgett (LB) films of relevant systems. Background studies on palmitic acid (PA) are presented which provide insight into the phase behavior of the fatty acid and to elucidate factors contributing to the final film structure of supported two-dimensional films. These studies are then extended to model systems containing both PA and a truncated form of natural SP-B (SP-B1--25). Confocal microscopy studies are conducted on these films to provide a macroscopic scale picture of peptide concentration dependence. Evidence is presented indicating a bimodal dependence in the phase behavior of the films with peptide concentration. Further, the results suggest the addition of 11 wt.% SP-B1--25 to monolayers of PA induces large density fluctuations in the membrane over macroscopic length scales, a signature of critical behavior. These experiments on the macroscopic scale are extended to the single molecule level by exploiting the unique nature of the electric fields present at the NSOM tip aperture. Single molecule fluorescence NSOM measurements reveal the orientation distribution of single fluorescent probe molecules doped into LB films of DPPC. The data indicate that the environment of the probe molecules becomes more ordered as surface pressure is increased, as would be expected for lipid films of DPPC. Finally, these measurements will be further supplemented through a new technique presented here which combines attributes from NSOM and fluorescence resonance energy transfer (FRET) to increase optical imaging capabilities in both the lateral and axial directions. (Abstract shortened by UMI.)
590 $a School code: 0099.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Physical. $3 560527
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Animal Physiology. $3 1017835
690 $a 0486
690 $a 0494
690 $a 0307
690 $a 0433
710 2 0 $a University of Kansas. $3 626626
773 0 $t Dissertation Abstracts International $g 63-11B.
790 1 0 $a Dunn, Robert, $e advisor
790 $a 0099
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3071142