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Dissection of the11q13 amplicon in o...

Huang, Xin.

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Dissection of the11q13 amplicon in oral cancer cells.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Dissection of the11q13 amplicon in oral cancer cells./
Author:	Huang, Xin.
Description:	138 p.
Notes:	Source: Dissertation Abstracts International, Volume: 63-10, Section: B, page: 4606.
Contained By:	Dissertation Abstracts International63-10B.
Subject:	Health Sciences, Oncology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3066957
ISBN:	0493863664

Dissection of the11q13 amplicon in oral cancer cells.
Huang, Xin.

Dissection of the11q13 amplicon in oral cancer cells. - 138 p.

Source: Dissertation Abstracts International, Volume: 63-10, Section: B, page: 4606.

Thesis (Ph.D.)--University of Pittsburgh, 2002.

Amplification of chromosomal band 11q13 is a common event in human cancer. It has been reported in approximately 45% of head and neck carcinomas, as well as in other malignancies including esophagus, breast, liver, and bladder cancer. In an effort to further characterize the 11q13 amplicon in oral cancer, a new technique called quantitative microsatellite analysis was utilized to fine map the 11q13 amplicon in 30 oral squamous cell carcinoma cell lines. 11q13 amplification was observed in 14/30 (47%) of cell lines examined. A 5-Mb physical map covering the 11q13 amplicon core was constructed in silico and the full DNA sequence of the mapped region is available. The core of the amplicon maps to a 1.5-Mb region containing the CCND1 gene as well as other seven known genes. Furthermore, the proximal boundary of the 11q13 amplicon is located to two intervals, 550 kb and 160 kb in size, respectively; the distal boundary of the 11q13 amplicon is mapped to a 250 kb interval. The clustering of the boundary breakpoints suggests the presence of as yet unidentified breakage hotspots or fragile sites and is consistent with a breakage-fusion-bridge cycle model for 11q13 amplification. In addition, three new EST clusters that are located at the peak of the amplicon close to CCND1 are identified. The expression level of three new ESTs, as well as CCND1 and OCIM (a candidate oncogene mapping to 11q13 amplicon core) are examined, using Northern blots and/or TaqMan QRT-PCR. It is found that CCND1, OCIM and one of the ESTs are frequently overexpressed in cell lines with 11q13 amplification. The full-length gene, TAOS1 (tumor amplified and overexpressed sequence 1), which is corresponding to the overexpressed EST, is cloned. TAOS1 encodes a 137 amino acid protein with a molecular weight of 15.4 kDa. TAOS1 amino acid sequence shares 77% homology with a previously described mouse gene, but the function of this gene is currently unknown. The coding region of the TAOS1 gene is cloned into a mammalian gene expression vector and is successfully expressed in the 293 cells. Immunostaining shows that the TAOS1 protein is located in cytoplasmic vesicles.

ISBN: 0493863664Subjects--Topical Terms:

1018566
Health Sciences, Oncology.

Dissection of the11q13 amplicon in oral cancer cells.
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520 $a Amplification of chromosomal band 11q13 is a common event in human cancer. It has been reported in approximately 45% of head and neck carcinomas, as well as in other malignancies including esophagus, breast, liver, and bladder cancer. In an effort to further characterize the 11q13 amplicon in oral cancer, a new technique called quantitative microsatellite analysis was utilized to fine map the 11q13 amplicon in 30 oral squamous cell carcinoma cell lines. 11q13 amplification was observed in 14/30 (47%) of cell lines examined. A 5-Mb physical map covering the 11q13 amplicon core was constructed in silico and the full DNA sequence of the mapped region is available. The core of the amplicon maps to a 1.5-Mb region containing the CCND1 gene as well as other seven known genes. Furthermore, the proximal boundary of the 11q13 amplicon is located to two intervals, 550 kb and 160 kb in size, respectively; the distal boundary of the 11q13 amplicon is mapped to a 250 kb interval. The clustering of the boundary breakpoints suggests the presence of as yet unidentified breakage hotspots or fragile sites and is consistent with a breakage-fusion-bridge cycle model for 11q13 amplification. In addition, three new EST clusters that are located at the peak of the amplicon close to CCND1 are identified. The expression level of three new ESTs, as well as CCND1 and OCIM (a candidate oncogene mapping to 11q13 amplicon core) are examined, using Northern blots and/or TaqMan QRT-PCR. It is found that CCND1, OCIM and one of the ESTs are frequently overexpressed in cell lines with 11q13 amplification. The full-length gene, TAOS1 (tumor amplified and overexpressed sequence 1), which is corresponding to the overexpressed EST, is cloned. TAOS1 encodes a 137 amino acid protein with a molecular weight of 15.4 kDa. TAOS1 amino acid sequence shares 77% homology with a previously described mouse gene, but the function of this gene is currently unknown. The coding region of the TAOS1 gene is cloned into a mammalian gene expression vector and is successfully expressed in the 293 cells. Immunostaining shows that the TAOS1 protein is located in cytoplasmic vesicles.
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