東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Biomaterial-dependent neutrophil mob...

Zhou, Yue.

Linked to FindBook

Google Book

Amazon

博客來

Biomaterial-dependent neutrophil mobility and phagocytosis of bacteria in the mechanism of cardiovascular implant infection.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Biomaterial-dependent neutrophil mobility and phagocytosis of bacteria in the mechanism of cardiovascular implant infection./
Author:	Zhou, Yue.
Description:	200 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 6196.
Contained By:	Dissertation Abstracts International64-12B.
Subject:	Engineering, Biomedical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3118159

Biomaterial-dependent neutrophil mobility and phagocytosis of bacteria in the mechanism of cardiovascular implant infection.
Zhou, Yue.

Biomaterial-dependent neutrophil mobility and phagocytosis of bacteria in the mechanism of cardiovascular implant infection. - 200 p.

Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 6196.

Thesis (Ph.D.)--Case Western Reserve University, 2004.

Infection is a potentially serious complication and a major impediment to the long-term clinical success of implanted cardiovascular devices. This thesis examined the hypothesis that foreign-body biomaterials and surface-associated host proteins cause attenuations in neutrophil mobility and phagocytosis that increase the probability for cardiovascular implant-related infection. Reduced neutrophil mobility and phagocytosis may prevent access and effective killing of bacteria, giving the bacteria sufficient opportunity to produce a biofilm.Subjects--Topical Terms:

1017684
Engineering, Biomedical.

Biomaterial-dependent neutrophil mobility and phagocytosis of bacteria in the mechanism of cardiovascular implant infection.
LDR:03646nmm 2200301 4500 001 1865015
005 20041216133912.5
008 130614s2004 eng d
035 $a (UnM)AAI3118159
035 $a AAI3118159
040 $a UnM $c UnM
100 1 $a Zhou, Yue. $3 1952479
245 1 0 $a Biomaterial-dependent neutrophil mobility and phagocytosis of bacteria in the mechanism of cardiovascular implant infection.
300 $a 200 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 6196.
500 $a Adviser: Roger E. Marchant.
502 $a Thesis (Ph.D.)--Case Western Reserve University, 2004.
520 $a Infection is a potentially serious complication and a major impediment to the long-term clinical success of implanted cardiovascular devices. This thesis examined the hypothesis that foreign-body biomaterials and surface-associated host proteins cause attenuations in neutrophil mobility and phagocytosis that increase the probability for cardiovascular implant-related infection. Reduced neutrophil mobility and phagocytosis may prevent access and effective killing of bacteria, giving the bacteria sufficient opportunity to produce a biofilm.
520 $a To address the hypothesis, microscopic methods were used to provide quantitative comparisons of neutrophil mobility and phagocytic function on materials with different surface properties.
520 $a To test human neutrophil mobility, three materials were selected. These materials include a clinically relevant polyurethane (Chronoflex AR), a hydrophobic model surface consisting of an octadecyltrichlorosilane (OTS) self-assembled monolayer (SAM), and a glass reference material. Surface dependent neutrophil mobility was evaluated by time-lapse optical microscopy, confocal microscopy and atomic force microscopy (AFM). Our results show that material surface properties and surface adsorbed proteins are important in neutrophil mobility induced by N-formylmethionyl-leucyl-phenylalanine (fMLP) stimulation. Without plasma protein adsorption, neutrophil mobility increased with increasing material surface hydrophobicity from glass, to polyurethane to OTS. With plasma protein adsorption, neutrophil mobility decreased with increasing material surface hydrophobicity from glass, Polyurethane to OTS surfaces. The decrease in neutrophil mobility under stimulation was directly related to increased cell spreading on the material substrates.
520 $a Neutrophil phagocytosis of Staphylococcus epidermidis on the three materials was recorded using time-lapse optical microscopy and compared based on the rate of bacteria ingestion. The morphology of adherent neutrophils was analyzed by fluorescence confocal microscopy. Our results show that on the materials without adsorbed plasma protein, neutrophil phagocytic ability increases from hydrophilic glass to hydrophobic OTS. In the presence of adsorbed plasma protein, neutrophil phagocytic ability increased on all the three materials, but was highest on the polyurethane. The results suggest that material surface properties and the surface adsorbed plasma proteins significantly affect phagocytic ability of surface adherent neutrophils.
520 $a The results from this thesis may be of value in designing biomaterials surface that improve bactericidal function of neutrophil and reduce implant-associated infection.
590 $a School code: 0042.
650 4 $a Engineering, Biomedical. $3 1017684
690 $a 0541
710 2 0 $a Case Western Reserve University. $3 1017714
773 0 $t Dissertation Abstracts International $g 64-12B.
790 1 0 $a Marchant, Roger E., $e advisor
790 $a 0042
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3118159