東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Effect of transcriptional parameters...

Koduvayur, Sujatha P.

Linked to FindBook

Google Book

Amazon

博客來

Effect of transcriptional parameters on the folding of the Tetrahymena group I intron.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Effect of transcriptional parameters on the folding of the Tetrahymena group I intron./
Author:	Koduvayur, Sujatha P.
Description:	126 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 5932.
Contained By:	Dissertation Abstracts International64-12B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3114753

Effect of transcriptional parameters on the folding of the Tetrahymena group I intron.
Koduvayur, Sujatha P.

Effect of transcriptional parameters on the folding of the Tetrahymena group I intron. - 126 p.

Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 5932.

Thesis (Ph.D.)--University of Maryland, College Park, 2004.

Differential elongation rates and pausing patterns of polymerases are known to affect cotranscriptional folding of RNAs. The mechanism of coupling is not well studied. This study evaluates whether these factors contribute to the 20--50 fold splicing enhancement seen in vivo for the Tetrahymena group I intron. The splicing rates of T7 and Escherichia coli (E. coli) RNA polymerase (RNAP) transcripts were compared in vitro and in E. coli. T7 RNAP is a rapid and highly processive polymerase, while E. coli RNAP elongates transcripts more slowly. In a collaborative study with Scott Jackson, the splicing of transcripts of eukaryotic polymerase I and II (pol I and pol II) were compared in Saccharomyces cerevisiae. As the stability of nascent structures can be altered by changing the sequence of transcribed RNA, I have also studied the effect of rRNA exons on the folding of this RNA by comparing the splicing rates of pre-RNAs with E. coli and Tetrahymena thermophila domain IV rRNA exons in vitro and E. coli. In yeast, S. cerevisiae and T. thermophila rRNA, and GFP mRNA exon sequences were compared.Subjects--Topical Terms:

1017719
Biology, Molecular.

Effect of transcriptional parameters on the folding of the Tetrahymena group I intron.
LDR:03200nmm 2200313 4500 001 1865002
005 20041216133910.5
008 130614s2004 eng d
035 $a (UnM)AAI3114753
035 $a AAI3114753
040 $a UnM $c UnM
100 1 $a Koduvayur, Sujatha P. $3 1952466
245 1 0 $a Effect of transcriptional parameters on the folding of the Tetrahymena group I intron.
300 $a 126 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 5932.
500 $a Chairs: Sarah A. Woodson; Douglas Julin.
502 $a Thesis (Ph.D.)--University of Maryland, College Park, 2004.
520 $a Differential elongation rates and pausing patterns of polymerases are known to affect cotranscriptional folding of RNAs. The mechanism of coupling is not well studied. This study evaluates whether these factors contribute to the 20--50 fold splicing enhancement seen in vivo for the Tetrahymena group I intron. The splicing rates of T7 and Escherichia coli (E. coli) RNA polymerase (RNAP) transcripts were compared in vitro and in E. coli. T7 RNAP is a rapid and highly processive polymerase, while E. coli RNAP elongates transcripts more slowly. In a collaborative study with Scott Jackson, the splicing of transcripts of eukaryotic polymerase I and II (pol I and pol II) were compared in Saccharomyces cerevisiae. As the stability of nascent structures can be altered by changing the sequence of transcribed RNA, I have also studied the effect of rRNA exons on the folding of this RNA by comparing the splicing rates of pre-RNAs with E. coli and Tetrahymena thermophila domain IV rRNA exons in vitro and E. coli. In yeast, S. cerevisiae and T. thermophila rRNA, and GFP mRNA exon sequences were compared.
520 $a My E. coli and in vitro comparative study reveals that co-transcriptional folding is the reason for the rate enhancement seen in vivo for this RNA. For E. coli RNAP transcripts this is effected by the template-dependent, site-specific pausing of the polymerase along the template. For the pre-RNA with Tetrahymena rRNA exons this is effected by the low stability of nascent structures that enable rapid rearrangement of non-native structures into native ones.
520 $a In yeast, we find that both the polymerase and the RNA processing events affect folding of this intron. Pol I transcripts splice 10-fold better than their pol II counterparts and mRNA processing events retard splicing of short pol II transcripts by 10-fold. Moreover, in yeast, only mutations that increase the fraction of misfolded intermediates are rescued but not those that destabilize the native structure. This folding facilitation is however not dependent on the presence of longer rRNA exons in yeast as it is in E. coli . This might be indicative of a different folding facilitatory mechanism in yeast from that seen in E. coli.
590 $a School code: 0117.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Biophysics, General. $3 1019105
690 $a 0307
690 $a 0487
690 $a 0786
710 2 0 $a University of Maryland, College Park. $3 657686
773 0 $t Dissertation Abstracts International $g 64-12B.
790 1 0 $a Woodson, Sarah A., $e advisor
790 1 0 $a Julin, Douglas, $e advisor
790 $a 0117
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3114753