東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Development of whole genome optical ...

Lin, Jieyi.

Linked to FindBook

Google Book

Amazon

博客來

Development of whole genome optical mapping and its application to the analysis of Deinococcus radiodurans, Escherichia coli and Homo sapiens genomes.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Development of whole genome optical mapping and its application to the analysis of Deinococcus radiodurans, Escherichia coli and Homo sapiens genomes./
Author:	Lin, Jieyi.
Description:	213 p.
Notes:	Source: Dissertation Abstracts International, Volume: 60-05, Section: B, page: 1967.
Contained By:	Dissertation Abstracts International60-05B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9930204
ISBN:	0599301821

Development of whole genome optical mapping and its application to the analysis of Deinococcus radiodurans, Escherichia coli and Homo sapiens genomes.
Lin, Jieyi.

Development of whole genome optical mapping and its application to the analysis of Deinococcus radiodurans, Escherichia coli and Homo sapiens genomes. - 213 p.

Source: Dissertation Abstracts International, Volume: 60-05, Section: B, page: 1967.

Thesis (Ph.D.)--New York University, 1999.

Optical Mapping (OM) is an approach for the rapid construction of ordered restriction maps from individual surface-mounted DNA molecules following endonuclease digestion. Whole genome OM was developed and applied to the analysis of E. coli K-12, D. radiodurans, pathogenic E. coli O157:H7 and Homo sapiens genomes. An Nhe I map of E. coli K12 M1665 (4.6 Mb) was constructed by OM. The 150-fragment optical map had a relative sizing error of 5 per cent for fragments >5 kb. An Nhe I map of Deinococcus radiodurans (3.1 Mb) genome was generated both by a manual map alignment process and by Gentig, a map construction program based on Bayesian inference. A second Deinococcus radiodurans chromosome (416 kb) was discovered using OM. An approach was developed to link sequences to Optical Maps mediated by Southern blotting, which can be used to place any sequence, including a single sequence read on an Optical Map. Whole genome Optical Maps provided scaffolds for facilitated sequence assembly, verification and gap closure in whole genome sequencing projects of Deinococcus radiodurans and E. coli O157:H7. The comparison of the Nhe I Map of E. coli O157:H7 with that of E. coli K-12 facilitated strain variation studies. A pilot project for the construction of a high-resolution reference map of the whole human genome was carried out. The preliminary data corresponded well with the statistical model proposed by Lander and Waterman. Sample preparation methods for OM was applied in Scanning Force Microscopy (SFM) to examine surface mounted DNA molecules and combine OM with SFM. Finally, Scanning Electron Microscopy was used to study electronic charge distribution on OM surfaces to provide a theoretical basis for improving surface treatment conditions and engineering OM surfaces by nano-fabrication.

ISBN: 0599301821Subjects--Topical Terms:

1017730
Biology, Genetics.

Development of whole genome optical mapping and its application to the analysis of Deinococcus radiodurans, Escherichia coli and Homo sapiens genomes.
LDR:02795nmm 2200289 4500 001 1862001
005 20041215074546.5
008 130614s1999 eng d
020 $a 0599301821
035 $a (UnM)AAI9930204
035 $a AAI9930204
040 $a UnM $c UnM
100 1 $a Lin, Jieyi. $3 1949574
245 1 0 $a Development of whole genome optical mapping and its application to the analysis of Deinococcus radiodurans, Escherichia coli and Homo sapiens genomes.
300 $a 213 p.
500 $a Source: Dissertation Abstracts International, Volume: 60-05, Section: B, page: 1967.
500 $a Adviser: David C. Schwartz.
502 $a Thesis (Ph.D.)--New York University, 1999.
520 $a Optical Mapping (OM) is an approach for the rapid construction of ordered restriction maps from individual surface-mounted DNA molecules following endonuclease digestion. Whole genome OM was developed and applied to the analysis of E. coli K-12, D. radiodurans, pathogenic E. coli O157:H7 and Homo sapiens genomes. An Nhe I map of E. coli K12 M1665 (4.6 Mb) was constructed by OM. The 150-fragment optical map had a relative sizing error of 5 per cent for fragments >5 kb. An Nhe I map of Deinococcus radiodurans (3.1 Mb) genome was generated both by a manual map alignment process and by Gentig, a map construction program based on Bayesian inference. A second Deinococcus radiodurans chromosome (416 kb) was discovered using OM. An approach was developed to link sequences to Optical Maps mediated by Southern blotting, which can be used to place any sequence, including a single sequence read on an Optical Map. Whole genome Optical Maps provided scaffolds for facilitated sequence assembly, verification and gap closure in whole genome sequencing projects of Deinococcus radiodurans and E. coli O157:H7. The comparison of the Nhe I Map of E. coli O157:H7 with that of E. coli K-12 facilitated strain variation studies. A pilot project for the construction of a high-resolution reference map of the whole human genome was carried out. The preliminary data corresponded well with the statistical model proposed by Lander and Waterman. Sample preparation methods for OM was applied in Scanning Force Microscopy (SFM) to examine surface mounted DNA molecules and combine OM with SFM. Finally, Scanning Electron Microscopy was used to study electronic charge distribution on OM surfaces to provide a theoretical basis for improving surface treatment conditions and engineering OM surfaces by nano-fabrication.
590 $a School code: 0146.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
690 $a 0369
690 $a 0410
690 $a 0307
710 2 0 $a New York University. $3 515735
773 0 $t Dissertation Abstracts International $g 60-05B.
790 1 0 $a Schwartz, David C., $e advisor
790 $a 0146
791 $a Ph.D.
792 $a 1999
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9930204