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DNA from dirt: Molecular analysis of...

Chandler, Darrell P.

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DNA from dirt: Molecular analysis of a deep subsurface microbial community and its response to environmental perturbation.

Record Type:	Electronic resources : Monograph/item
Title/Author:	DNA from dirt: Molecular analysis of a deep subsurface microbial community and its response to environmental perturbation./
Author:	Chandler, Darrell P.
Description:	96 p.
Notes:	Source: Dissertation Abstracts International, Volume: 58-08, Section: B, page: 4032.
Contained By:	Dissertation Abstracts International58-08B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9806715
ISBN:	0591569515

DNA from dirt: Molecular analysis of a deep subsurface microbial community and its response to environmental perturbation.
Chandler, Darrell P.

DNA from dirt: Molecular analysis of a deep subsurface microbial community and its response to environmental perturbation. - 96 p.

Source: Dissertation Abstracts International, Volume: 58-08, Section: B, page: 4032.

Thesis (Ph.D.)--Washington State University, 1996.

Culture-independent nucleic acid-based methods were used to identify members of a deep subsurface microbial community from the Department of Energy's Hanford site and the response of this community to environmental perturbation. Two contiguous core sections from a buried paleosol (

ISBN: 0591569515Subjects--Topical Terms:

1017734
Biology, Microbiology.

DNA from dirt: Molecular analysis of a deep subsurface microbial community and its response to environmental perturbation.
LDR:03106nmm 2200277 4500 001 1861997
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020 $a 0591569515
035 $a (UnM)AAI9806715
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040 $a UnM $c UnM
100 1 $a Chandler, Darrell P. $3 1949570
245 1 0 $a DNA from dirt: Molecular analysis of a deep subsurface microbial community and its response to environmental perturbation.
300 $a 96 p.
500 $a Source: Dissertation Abstracts International, Volume: 58-08, Section: B, page: 4032.
500 $a Chair: Michael L. Kahn.
502 $a Thesis (Ph.D.)--Washington State University, 1996.
520 $a Culture-independent nucleic acid-based methods were used to identify members of a deep subsurface microbial community from the Department of Energy's Hanford site and the response of this community to environmental perturbation. Two contiguous core sections from a buried paleosol ( $\ sim $1 88 m below the surface) were incubated as an intact core or homogenized sediment at the in situ temperature for up to 21 weeks. Total DNA and RNA was isolated from these low-biomass ( $\ sim $1 0 $\ sp{4.5} $ cells g $\ sp{-1}) $ sediments. Over 2800 16S rDNA clones were recovered; 865 were characterized by RFLP analysis. Analysis of clone libraries derived from undiluted or diluted templates suggest that the composition and distribution of clones within a 16S rDNA library can be affected by starting template concentration. A successional response of microbial types was observed in clone libraries from the two sediment treatments, with 98 different eubacterial RFLP signatures identified. Only 12 clone types appeared in both libraries. Results suggest that clone libraries can be qualitative and semi-quantitative indicators of community structure and dynamics. Clone-specific RFLP patterns were compared to 16S rDNA RFLP profiles of 100 morphologically distinct isolates recovered from the same sediment and treatments. 64% of the isolates were observed in the clone libraries, suggesting that the clone libraries are fairly representative of known microbial diversity in these sediments. The molecular phylogeny of 278 clones was also examined. Most clones represent undescribed relatives of common soil organisms. A novel clade of Thiobacillus clones was discovered, along with 2 eubacterial sequences most closely associated with Thermus sp. and Deinococcus radiodurans. A deeply-branching, novel group of Crenarchaeota was also identified and shown to be active members of the microbial community, calling into question the notion that all Crenarchaea derive from thermophilic ancestry. In summary, this dissertation provides a much-needed framework for future analyses of microbial communities impacted by contaminant plumes and other deep subsurface environmental perturbations.
590 $a School code: 0251.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Ecology. $3 1017726
690 $a 0410
690 $a 0329
710 2 0 $a Washington State University. $3 678588
773 0 $t Dissertation Abstracts International $g 58-08B.
790 1 0 $a Kahn, Michael L., $e advisor
790 $a 0251
791 $a Ph.D.
792 $a 1996
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9806715