東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

AN ULTRASTRUCTURAL AND BIOCHEMICAL S...

THOMPSON, BRADLEY GEORGE.

Linked to FindBook

Google Book

Amazon

博客來

AN ULTRASTRUCTURAL AND BIOCHEMICAL STUDY OF THE CELL ENVELOPE OF DEINOCOCCUS RADIODURANS STRAIN SARK.

Record Type:	Electronic resources : Monograph/item
Title/Author:	AN ULTRASTRUCTURAL AND BIOCHEMICAL STUDY OF THE CELL ENVELOPE OF DEINOCOCCUS RADIODURANS STRAIN SARK./
Author:	THOMPSON, BRADLEY GEORGE.
Notes:	Source: Dissertation Abstracts International, Volume: 43-08, Section: B, page: 2457.
Contained By:	Dissertation Abstracts International43-08B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NK52991
ISBN:	0315050977

AN ULTRASTRUCTURAL AND BIOCHEMICAL STUDY OF THE CELL ENVELOPE OF DEINOCOCCUS RADIODURANS STRAIN SARK.
THOMPSON, BRADLEY GEORGE.

AN ULTRASTRUCTURAL AND BIOCHEMICAL STUDY OF THE CELL ENVELOPE OF DEINOCOCCUS RADIODURANS STRAIN SARK.

Source: Dissertation Abstracts International, Volume: 43-08, Section: B, page: 2457.

Thesis (Ph.D.)--The University of Western Ontario (Canada), 1982.

A descriptive study of the cell envelope of Deinococcus radiodurans strain Sark and an exploration of the major components of the cell envelope was undertaken. The cell envelope consisted of six layers as seen in thin sections: a plasma membrane, 8.0nm thick; a peptidoglycan layer, 30nm thick; a compartmentalized layer, 20 - 75nm thick; an outer membrane, 6.5nm thick; an electron transparent zone, 10nm thick, and a hexagonal surface array, 2 - 3nm thick.

ISBN: 0315050977Subjects--Topical Terms:

1017734
Biology, Microbiology.

AN ULTRASTRUCTURAL AND BIOCHEMICAL STUDY OF THE CELL ENVELOPE OF DEINOCOCCUS RADIODURANS STRAIN SARK.
LDR:03340nmm 2200253 4500 001 1861975
005 20041215074543.5
008 130614s1982 d
020 $a 0315050977
035 $a (UnM)AAINK52991
035 $a AAINK52991
040 $a UnM $c UnM
100 1 $a THOMPSON, BRADLEY GEORGE. $3 1949550
245 1 3 $a AN ULTRASTRUCTURAL AND BIOCHEMICAL STUDY OF THE CELL ENVELOPE OF DEINOCOCCUS RADIODURANS STRAIN SARK.
500 $a Source: Dissertation Abstracts International, Volume: 43-08, Section: B, page: 2457.
502 $a Thesis (Ph.D.)--The University of Western Ontario (Canada), 1982.
520 $a A descriptive study of the cell envelope of Deinococcus radiodurans strain Sark and an exploration of the major components of the cell envelope was undertaken. The cell envelope consisted of six layers as seen in thin sections: a plasma membrane, 8.0nm thick; a peptidoglycan layer, 30nm thick; a compartmentalized layer, 20 - 75nm thick; an outer membrane, 6.5nm thick; an electron transparent zone, 10nm thick, and a hexagonal surface array, 2 - 3nm thick.
520 $a The outer cell envelope layers formed blebs throughout the growth cycle, which were shed as large vesicles (0.5(mu) to 3.5(mu) in diameter) from 5% of the cell population. Instability of the compartmentalized layer immediately beneath the outer membrane was the cause of the vesicle production. This instability was accentuated by treatment with 10% NaCl, which released the outer cell envelope layers as vesicles from all of the cell population without disrupting the peptidoglycan layer. Any remaining portions of the compartmentalized layer could be removed from these vesicles by 3M urea, which yielded purified outer membrane vesicles. The outer membrane vesicles were 0.5(mu) to 3.5(mu) in diameter, had a density of 1.27g/cm('3), and were 16% (w/w) lipid, 80% (w/w) protein, and 3.9% (w/w) non chloroform-methanol extractable (non-lipid) carbohydrate. There were 43 protein bands present on polyacrylamide gels of the outer membrane; two were identified as the hexagonal surface array proteins. No lipopolysaccharide-like compound was found in the outer membrane.
520 $a The hexagonal surface array was isolated by digesting isolated outer membrane with 2% sodium dodecyl sulphate (SDS), which dissolved all non-array components. The isolated array consisted of a major 115,000 Dalton (D) protein and a minor 108,000D protein. The array could not be separated from the outer membrane by reagents that disrupt ionic bonds (pH, NaCl, LiCl, KCl), disulphid bonds (mercaptoethanol, dithiothreitol), hydrogen bonds (urea, guandine HCl), or by chelating agents (EDTA). Only disruption of the outer membrane with SDS, separated the outer membrane and the hexagonal surface array. This suggested that the hexagonal surface array was held onto the bacterial surface largely by non-specific (hydrophobic) interactions. The external surface of the hexagonal surface array was also found to be more hydrophilic than the surface of the outer membrane. . . . (Author's abstract exceeds stipulated maximum length. Discontinued here with permission of school.) UMI
590 $a School code: 0784.
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0410
710 2 0 $a The University of Western Ontario (Canada). $3 1017622
773 0 $t Dissertation Abstracts International $g 43-08B.
790 $a 0784
791 $a Ph.D.
792 $a 1982
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NK52991