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Development, testing and application...

Pan, Yan.

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Development, testing and application of an experimental system to study sensory neuron responses to tooth injury.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Development, testing and application of an experimental system to study sensory neuron responses to tooth injury./
Author:	Pan, Yan.
Description:	136 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-05, Section: B, page: 1967.
Contained By:	Dissertation Abstracts International64-05B.
Subject:	Biology, Anatomy. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3091792

Development, testing and application of an experimental system to study sensory neuron responses to tooth injury.
Pan, Yan.

Development, testing and application of an experimental system to study sensory neuron responses to tooth injury. - 136 p.

Source: Dissertation Abstracts International, Volume: 64-05, Section: B, page: 1967.

Thesis (Ph.D.)--The University of Mississippi Medical Center, 2003.

The dental pulp is a favorable model for studies of interactions between nociceptive sensory neurons and their peripheral target tissues. In the present study, we retrogradely labeled pulpal afferent neurons with an improved method that permits monitoring of changes in neuronal phenotype in response to controlled tooth injuries. The capacity of retrograde neuronal tracers to diffuse through dentinal tubules was exploited, thereby avoiding the severe injury to the pulp associated with previous tracer application methods. The strategy was to apply the durable fluorescent tracer, Fluoro-gold (FG), to exposed dentin in the floor of shallow cavities in molars in order to pre-label pulpal neurons in trigeminal ganglia of young adult Sprague-Dawley rats. A high percentage of pupal afferent neurons was retrogradely labeled by application of FG to exposed dentin. The FG fluorescent signal persisted in most labeled neurons for at least eight weeks, and these neurons maintained their peripheral projection to the dental pulp. Following tracer application to dentin, the pulp tissue appeared normal histologically, with the exception that a layer of reactive dentin was deposited at the pulp-dentin border beneath the shallow cavities. Thus, labeling of trigeminal neurons via dentinal tubules provides the basis for a useful model for precisely assessing properties of pulpal afferents in both quiescent and activated states. This new experimental system was used to demonstrate that the pulpal sensory innervation includes a population of NGF-responsive neurons that upregulate expression of CGRP and BDNF in response to tooth injury.Subjects--Topical Terms:

1021727
Biology, Anatomy.

Development, testing and application of an experimental system to study sensory neuron responses to tooth injury.
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035 $a AAI3091792
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100 1 $a Pan, Yan. $3 1949482
245 1 0 $a Development, testing and application of an experimental system to study sensory neuron responses to tooth injury.
300 $a 136 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-05, Section: B, page: 1967.
500 $a Adviser: John P. Naftel.
502 $a Thesis (Ph.D.)--The University of Mississippi Medical Center, 2003.
520 $a The dental pulp is a favorable model for studies of interactions between nociceptive sensory neurons and their peripheral target tissues. In the present study, we retrogradely labeled pulpal afferent neurons with an improved method that permits monitoring of changes in neuronal phenotype in response to controlled tooth injuries. The capacity of retrograde neuronal tracers to diffuse through dentinal tubules was exploited, thereby avoiding the severe injury to the pulp associated with previous tracer application methods. The strategy was to apply the durable fluorescent tracer, Fluoro-gold (FG), to exposed dentin in the floor of shallow cavities in molars in order to pre-label pulpal neurons in trigeminal ganglia of young adult Sprague-Dawley rats. A high percentage of pupal afferent neurons was retrogradely labeled by application of FG to exposed dentin. The FG fluorescent signal persisted in most labeled neurons for at least eight weeks, and these neurons maintained their peripheral projection to the dental pulp. Following tracer application to dentin, the pulp tissue appeared normal histologically, with the exception that a layer of reactive dentin was deposited at the pulp-dentin border beneath the shallow cavities. Thus, labeling of trigeminal neurons via dentinal tubules provides the basis for a useful model for precisely assessing properties of pulpal afferents in both quiescent and activated states. This new experimental system was used to demonstrate that the pulpal sensory innervation includes a population of NGF-responsive neurons that upregulate expression of CGRP and BDNF in response to tooth injury.
590 $a School code: 0805.
650 4 $a Biology, Anatomy. $3 1021727
650 4 $a Biology, Neuroscience. $3 1017680
650 4 $a Biology, Cell. $3 1017686
650 4 $a Engineering, Biomedical. $3 1017684
650 4 $a Health Sciences, Dentistry. $3 1019378
690 $a 0287
690 $a 0317
690 $a 0379
690 $a 0541
690 $a 0567
710 2 0 $a The University of Mississippi Medical Center. $3 1017852
773 0 $t Dissertation Abstracts International $g 64-05B.
790 1 0 $a Naftel, John P., $e advisor
790 $a 0805
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3091792