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The oligoguanidine transport system:...

Wright, Lee Robert.

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The oligoguanidine transport system: A novel approach to drug delivery.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The oligoguanidine transport system: A novel approach to drug delivery./
Author:	Wright, Lee Robert.
Description:	220 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-03, Section: B, page: 1255.
Contained By:	Dissertation Abstracts International64-03B.
Subject:	Chemistry, Organic. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3085243

The oligoguanidine transport system: A novel approach to drug delivery.
Wright, Lee Robert.

The oligoguanidine transport system: A novel approach to drug delivery. - 220 p.

Source: Dissertation Abstracts International, Volume: 64-03, Section: B, page: 1255.

Thesis (Ph.D.)--Stanford University, 2003.

The oligoguanidine transport system (OGTS) is a highly conserved system for enabling cellular entry into a wide variety of cell types and tissues. Uptake by this mechanism is apparently energy-dependent and appears to require at least six arginine residues or guanidinium groups. Until recently, the utility of this transport system was virtually unexplored in regard to promoting cellular uptake of therapeutic agents. The benefit of such a vehicle for drug delivery is two-fold; first, the nature of the transport peptide imparts on the attached cargo a dramatically enhanced aqueous solubility that enables drug administrations without the use of adjuvants or excipients; second it provides a non-invasive method for the delivery of potential therapeutic agents that could not otherwise cross the plasma membrane to interact with their subcellular targets.Subjects--Topical Terms:

516206
Chemistry, Organic.

The oligoguanidine transport system: A novel approach to drug delivery.
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035 $a (UnM)AAI3085243
035 $a AAI3085243
040 $a UnM $c UnM
100 1 $a Wright, Lee Robert. $3 1948128
245 1 4 $a The oligoguanidine transport system: A novel approach to drug delivery.
300 $a 220 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-03, Section: B, page: 1255.
500 $a Adviser: Paul A. Wender.
502 $a Thesis (Ph.D.)--Stanford University, 2003.
520 $a The oligoguanidine transport system (OGTS) is a highly conserved system for enabling cellular entry into a wide variety of cell types and tissues. Uptake by this mechanism is apparently energy-dependent and appears to require at least six arginine residues or guanidinium groups. Until recently, the utility of this transport system was virtually unexplored in regard to promoting cellular uptake of therapeutic agents. The benefit of such a vehicle for drug delivery is two-fold; first, the nature of the transport peptide imparts on the attached cargo a dramatically enhanced aqueous solubility that enables drug administrations without the use of adjuvants or excipients; second it provides a non-invasive method for the delivery of potential therapeutic agents that could not otherwise cross the plasma membrane to interact with their subcellular targets.
520 $a Application the OGTS transporters to Taxol provides an example of how this technology can be used to ameliorate issues related to drug formulation by increasing the compound's native water solubility without compromising its cellular uptake. In addition, the OGTS has been employed to enable the uptake of a water soluble, cell-impermeable PKC-epsilon peptide agonist, epsilonV1-7. Conjugation of the peptide drug to the transport peptide was accomplished through a readily reducible cysteine-cysteine disulfide bond. As anticipated, the cargo efficiently delivered and released both as evidenced by a dramatic reduction in hypoxia-related cell death.
520 $a Finally, attention was turned to the transport system itself and on how it might be further improved. In an effort to increase the number of head groups in contact with the membrane, perturbations were introduced to the peptide's primary structure in the form of proline residues. Molecular modeling of the resultant construct, RRPRRPRR, suggested that four or possibly five of the six head groups were capable of simultaneously interacting with the surface phosphate groups, a putative action required for uptake. Analysis of this compound in a FACS analysis showed that the proline substituted structure was not only capable of entering cells, but that it was superior to the parent heptamer.
590 $a School code: 0212.
650 4 $a Chemistry, Organic. $3 516206
650 4 $a Biology, Cell. $3 1017686
650 4 $a Chemistry, Pharmaceutical. $3 550957
690 $a 0490
690 $a 0379
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710 2 0 $a Stanford University. $3 754827
773 0 $t Dissertation Abstracts International $g 64-03B.
790 1 0 $a Wender, Paul A., $e advisor
790 $a 0212
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3085243