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Molecular marker variation and disea...

Westman, Anne Louise.

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Molecular marker variation and disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris in Brassica nigra and other crucifer species.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Molecular marker variation and disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris in Brassica nigra and other crucifer species./
Author:	Westman, Anne Louise.
Description:	168 p.
Notes:	Source: Dissertation Abstracts International, Volume: 58-12, Section: B, page: 6373.
Contained By:	Dissertation Abstracts International58-12B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9818280
ISBN:	0591698595

Molecular marker variation and disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris in Brassica nigra and other crucifer species.
Westman, Anne Louise.

Molecular marker variation and disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris in Brassica nigra and other crucifer species. - 168 p.

Source: Dissertation Abstracts International, Volume: 58-12, Section: B, page: 6373.

Thesis (Ph.D.)--Cornell University, 1998.

Weedy crucifer taxa may be sources of useful traits for Brassica crop improvement, but they have seldom been evaluated, and many are thought to contain little genetic variation. Few molecular markers have been developed to describe variation in these taxa, and little is known about its distribution. In greenhouse trials, 190 entries (genebank accessions and weed populations) of 24 crucifer species were evaluated for disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris. Brassica nigra, a weed and minor crop, was represented by entries from four regions: Europe/North Africa (its native range), India, Ethiopia, and North America. To assess the potential for using molecular markers across crucifer taxa, 30 primer pairs designed for simple sequence repeats (SSRs) in Arabidopsis thaliana were evaluated for amplification and polymorphism in six Brassica species. Five markers based on crucifer SSRs were used to describe variation in 32 B. nigra entries from the four regions.

ISBN: 0591698595Subjects--Topical Terms:

1017730
Biology, Genetics.

Molecular marker variation and disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris in Brassica nigra and other crucifer species.
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008 130614s1998 eng d
020 $a 0591698595
035 $a (UnM)AAI9818280
035 $a AAI9818280
040 $a UnM $c UnM
100 1 $a Westman, Anne Louise. $3 1943504
245 1 0 $a Molecular marker variation and disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris in Brassica nigra and other crucifer species.
300 $a 168 p.
500 $a Source: Dissertation Abstracts International, Volume: 58-12, Section: B, page: 6373.
500 $a Adviser: William Ronnie Coffman.
502 $a Thesis (Ph.D.)--Cornell University, 1998.
520 $a Weedy crucifer taxa may be sources of useful traits for Brassica crop improvement, but they have seldom been evaluated, and many are thought to contain little genetic variation. Few molecular markers have been developed to describe variation in these taxa, and little is known about its distribution. In greenhouse trials, 190 entries (genebank accessions and weed populations) of 24 crucifer species were evaluated for disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris. Brassica nigra, a weed and minor crop, was represented by entries from four regions: Europe/North Africa (its native range), India, Ethiopia, and North America. To assess the potential for using molecular markers across crucifer taxa, 30 primer pairs designed for simple sequence repeats (SSRs) in Arabidopsis thaliana were evaluated for amplification and polymorphism in six Brassica species. Five markers based on crucifer SSRs were used to describe variation in 32 B. nigra entries from the four regions.
520 $a Disease reaction was highly variable between and within species. Resistance to A. brassicicola was variable in Camelina sativa, a species reported as highly resistant to Alternaria pathogens. In B. nigra, regional differences in disease reaction, plant phenology, and leaf trichomes were apparent. Most B. nigra entries rated as disease resistant were North American weed populations. In Brassica, 17 A. thaliana SSR primer pairs generated PCR products that were selected as likely to contain SSRs. However, not all of the products hybridized to SSR probes, and some that hybridized may have been single-primer products. Markers based on A. thaliana SSRs may be applicable across taxa, but tests are necessary to determine the nature of the products. Five SSR-based markers were informative descriptors of variation in B. nigra. Differences between regions and entries within regions were significant, but they explained less than half of the total variation. Ethiopian entries formed a distinct group. European and North American entries contained the most variation and were generally grouped together. Unique variants were present in North American weed populations. Between the populations, neither disease reaction nor marker variation were correlated with geographic distance. In summary, noncrop crucifers contained considerable variation for disease reaction and molecular markers. Markers based on SSRs can be applied across crucifer taxa, and patterns of variation they describe can help us more effectively conserve and use crucifer genetic resources.
590 $a School code: 0058.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Agriculture, Plant Culture. $3 1018669
650 4 $a Biology, Botany. $3 1017825
650 4 $a Agriculture, Plant Pathology. $3 1028950
690 $a 0369
690 $a 0479
690 $a 0309
690 $a 0480
710 2 0 $a Cornell University. $3 530586
773 0 $t Dissertation Abstracts International $g 58-12B.
790 1 0 $a Coffman, William Ronnie, $e advisor
790 $a 0058
791 $a Ph.D.
792 $a 1998
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9818280