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Determination of lactose by reversed...

Sexton, Danessa.

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Determination of lactose by reversed-phase high-performance liquid chromatography.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Determination of lactose by reversed-phase high-performance liquid chromatography./
Author:	Sexton, Danessa.
Description:	94 p.
Notes:	Source: Masters Abstracts International, Volume: 42-05, page: 1713.
Contained By:	Masters Abstracts International42-05.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1418655
ISBN:	0496234285

Determination of lactose by reversed-phase high-performance liquid chromatography.
Sexton, Danessa.

Determination of lactose by reversed-phase high-performance liquid chromatography. - 94 p.

Source: Masters Abstracts International, Volume: 42-05, page: 1713.

Thesis (M.S.)--East Tennessee State University, 2004.

Lactose is the common disaccharide found in dairy products. It can, however, be a source of discomfort for those whom are lactose intolerant. Therefore, it is necessary to accurately, reliably, and efficiently determine the lactose content of foods. The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300--360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile phase. A linear response of peak area to lactose concentration of 0.018 mg/mL to 0.36 mg/mL was obtained with an estimated detection limit of 0.0036 mg/mL lactose concentration. The reproducibility was established with a 4.95% relative standard deviation. The average recovery was 107%, and results were in strong agreement with the standard alkaline ferricyanide method, establishing accuracy.

ISBN: 0496234285Subjects--Topical Terms:

586156
Chemistry, Analytical.

Determination of lactose by reversed-phase high-performance liquid chromatography.
LDR:01807nmm 2200265 4500 001 1848770
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020 $a 0496234285
035 $a (UnM)AAI1418655
035 $a AAI1418655
040 $a UnM $c UnM
100 1 $a Sexton, Danessa. $3 1936768
245 1 0 $a Determination of lactose by reversed-phase high-performance liquid chromatography.
300 $a 94 p.
500 $a Source: Masters Abstracts International, Volume: 42-05, page: 1713.
500 $a Chair: Chu-Ngi Ho.
502 $a Thesis (M.S.)--East Tennessee State University, 2004.
520 $a Lactose is the common disaccharide found in dairy products. It can, however, be a source of discomfort for those whom are lactose intolerant. Therefore, it is necessary to accurately, reliably, and efficiently determine the lactose content of foods. The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300--360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile phase. A linear response of peak area to lactose concentration of 0.018 mg/mL to 0.36 mg/mL was obtained with an estimated detection limit of 0.0036 mg/mL lactose concentration. The reproducibility was established with a 4.95% relative standard deviation. The average recovery was 107%, and results were in strong agreement with the standard alkaline ferricyanide method, establishing accuracy.
590 $a School code: 0069.
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0486
710 2 0 $a East Tennessee State University. $3 1017802
773 0 $t Masters Abstracts International $g 42-05.
790 1 0 $a Ho, Chu-Ngi, $e advisor
790 $a 0069
791 $a M.S.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1418655