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REDOR NMR spectroscopy for the struc...

Cegelski, Lynette Susanne.

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REDOR NMR spectroscopy for the structural biologist: An investigation of whole cells, cell walls, and cell-surface proteins.

Record Type:	Electronic resources : Monograph/item
Title/Author:	REDOR NMR spectroscopy for the structural biologist: An investigation of whole cells, cell walls, and cell-surface proteins./
Author:	Cegelski, Lynette Susanne.
Description:	172 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-09, Section: B, page: 4589.
Contained By:	Dissertation Abstracts International65-09B.
Subject:	Chemistry, Physical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3147431
ISBN:	0496060252

REDOR NMR spectroscopy for the structural biologist: An investigation of whole cells, cell walls, and cell-surface proteins.
Cegelski, Lynette Susanne.

REDOR NMR spectroscopy for the structural biologist: An investigation of whole cells, cell walls, and cell-surface proteins. - 172 p.

Source: Dissertation Abstracts International, Volume: 65-09, Section: B, page: 4589.

Thesis (Ph.D.)--Washington University, 2004.

Solid-state NMR spectroscopy has been employed to address several important problems in structural biology that have remained unsolved despite the application of the full arsenal of modern analytical tools (x-ray crystallography, cryoelectron microscopy, radio-isotope labeling). NMR analysis of isotopically labeled S. aureus whole cells has demonstrated the mode of action of vancomycin. Furthermore, a molecular snapshot of a potent vancomycin analogue complexed to S. aureus whole cells and cell walls has emerged from REDOR NMR distance measurements performed in situ. A method for the efficient isotopic labeling of soybean suspension cells has been developed and, for the first time, tyrosine cross-links have been identified in intact plant cell walls. Thus, REDOR has been established as a tool to investigate cell-wall architecture, a complicated problem that has defied solution for decades, primarily because removal of components from the cell wall for identification often leads to degradative changes in their structure.

ISBN: 0496060252Subjects--Topical Terms:

560527
Chemistry, Physical.

REDOR NMR spectroscopy for the structural biologist: An investigation of whole cells, cell walls, and cell-surface proteins.
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100 1 $a Cegelski, Lynette Susanne. $3 1935814
245 1 0 $a REDOR NMR spectroscopy for the structural biologist: An investigation of whole cells, cell walls, and cell-surface proteins.
300 $a 172 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-09, Section: B, page: 4589.
500 $a Chairperson: Jacob Schaefer.
502 $a Thesis (Ph.D.)--Washington University, 2004.
520 $a Solid-state NMR spectroscopy has been employed to address several important problems in structural biology that have remained unsolved despite the application of the full arsenal of modern analytical tools (x-ray crystallography, cryoelectron microscopy, radio-isotope labeling). NMR analysis of isotopically labeled S. aureus whole cells has demonstrated the mode of action of vancomycin. Furthermore, a molecular snapshot of a potent vancomycin analogue complexed to S. aureus whole cells and cell walls has emerged from REDOR NMR distance measurements performed in situ. A method for the efficient isotopic labeling of soybean suspension cells has been developed and, for the first time, tyrosine cross-links have been identified in intact plant cell walls. Thus, REDOR has been established as a tool to investigate cell-wall architecture, a complicated problem that has defied solution for decades, primarily because removal of components from the cell wall for identification often leads to degradative changes in their structure.
520 $a In work with soybean cells, REDOR has also been employed to make the first whole-cell direct measure of atom-to-atom protein-RNA interfacial contact within the ribosome. The direct determination of photorespiration during photosynthesis has been made with REDOR on intact leaves of soybean plants labeled with 13CO2. Despite thirty years of work on this problem, the outlook for clear answers had remained "generally dismal." Finally, preliminary work has set the stage to obtain insights regarding the atomic-level details of receptor-binding interactions of the human adhesion protein L-selectin. A stable-transfectant capable of expressing more than ten milligrams per liter protein has been created to permit subsequent solid-state NMR characterization of L-selectin bound to ligands and inhibitors. REDOR is a tool that can be applied to any system, large and heterogeneous, irregular or regular, soluble or insoluble, and should be in the toolbox of every structural biologist.
590 $a School code: 0252.
650 4 $a Chemistry, Physical. $3 560527
650 4 $a Biophysics, General. $3 1019105
650 4 $a Biology, Microbiology. $3 1017734
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773 0 $t Dissertation Abstracts International $g 65-09B.
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790 $a 0252
791 $a Ph.D.
792 $a 2004
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