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Tissue remodeling and genomics of to...

Holliday, Sean R.

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Tissue remodeling and genomics of tooth eruption in the un-opposed mouse molar.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Tissue remodeling and genomics of tooth eruption in the un-opposed mouse molar./
Author:	Holliday, Sean R.
Description:	60 p.
Notes:	Source: Masters Abstracts International, Volume: 42-05, page: 1670.
Contained By:	Masters Abstracts International42-05.
Subject:	Health Sciences, Dentistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1418617
ISBN:	0496233904

Tissue remodeling and genomics of tooth eruption in the un-opposed mouse molar.
Holliday, Sean R.

Tissue remodeling and genomics of tooth eruption in the un-opposed mouse molar. - 60 p.

Source: Masters Abstracts International, Volume: 42-05, page: 1670.

Thesis (M.S.)--University of Illinois at Chicago, 2004.

Tooth eruption is a multifactorial process that involves the movement of existing tissues and the formation of new tissues coordinated by a complex set of genetic events. We have used the model of the un-opposed rodent molar to study morphologic and genetic mechanisms of tooth eruption. Following the extraction of left maxillary molar teeth in mice, mandibular molars were allowed to super-erupt. The mice were injected with fluorescent dyes. Mandible tissue blocks were prepared for ultrathin sections and fluorescent microscopy. Other specimens were prepared for RNA extraction, microarray analysis, and immunohistochemistry. Labeled tissue sections revealed significant differences in bone and cementum apposition between the experimental and the control side. Microarray analysis demonstrated significant increase in gene expression of collagen I, integrin beta5, and SPARC. The results from the microarray were verified using RT-PCR. The immunohistochemistry revealed increased levels of expression in the periodontal ligament of the un-opposed molar.

ISBN: 0496233904Subjects--Topical Terms:

1019378
Health Sciences, Dentistry.

Tissue remodeling and genomics of tooth eruption in the un-opposed mouse molar.
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100 1 $a Holliday, Sean R. $3 1935524
245 1 0 $a Tissue remodeling and genomics of tooth eruption in the un-opposed mouse molar.
300 $a 60 p.
500 $a Source: Masters Abstracts International, Volume: 42-05, page: 1670.
500 $a Adviser: Thomas Diekwisch.
502 $a Thesis (M.S.)--University of Illinois at Chicago, 2004.
520 $a Tooth eruption is a multifactorial process that involves the movement of existing tissues and the formation of new tissues coordinated by a complex set of genetic events. We have used the model of the un-opposed rodent molar to study morphologic and genetic mechanisms of tooth eruption. Following the extraction of left maxillary molar teeth in mice, mandibular molars were allowed to super-erupt. The mice were injected with fluorescent dyes. Mandible tissue blocks were prepared for ultrathin sections and fluorescent microscopy. Other specimens were prepared for RNA extraction, microarray analysis, and immunohistochemistry. Labeled tissue sections revealed significant differences in bone and cementum apposition between the experimental and the control side. Microarray analysis demonstrated significant increase in gene expression of collagen I, integrin beta5, and SPARC. The results from the microarray were verified using RT-PCR. The immunohistochemistry revealed increased levels of expression in the periodontal ligament of the un-opposed molar.
590 $a School code: 0799.
650 4 $a Health Sciences, Dentistry. $3 1019378
650 4 $a Biology, Anatomy. $3 1021727
650 4 $a Biology, Molecular. $3 1017719
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690 $a 0287
690 $a 0307
710 2 0 $a University of Illinois at Chicago. $3 1020478
773 0 $t Masters Abstracts International $g 42-05.
790 1 0 $a Diekwisch, Thomas, $e advisor
790 $a 0799
791 $a M.S.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1418617