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Estrogen regulation of gene expressi...

Park, Seongeun.

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Estrogen regulation of gene expression and analysis of the role of the coregulator, repressor of estrogen receptor activity (REA).

Record Type:	Electronic resources : Monograph/item
Title/Author:	Estrogen regulation of gene expression and analysis of the role of the coregulator, repressor of estrogen receptor activity (REA)./
Author:	Park, Seongeun.
Description:	148 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-04, Section: B, page: 1621.
Contained By:	Dissertation Abstracts International65-04B.
Subject:	Biology, Animal Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3131003
ISBN:	0496782517

Estrogen regulation of gene expression and analysis of the role of the coregulator, repressor of estrogen receptor activity (REA).
Park, Seongeun.

Estrogen regulation of gene expression and analysis of the role of the coregulator, repressor of estrogen receptor activity (REA). - 148 p.

Source: Dissertation Abstracts International, Volume: 65-04, Section: B, page: 1621.

Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004.

To understand estrogen regulation of gene expression and the role of the coregulator, repressor of estrogen receptor activity (REA), I analyzed the 5'-regulatory region of the Na+/H + exchanger regulatory factor/ezrin-radixin moesin binding protein 50 (NHE-RF/EBP50) gene and developed REA knockout mice to characterize the role of REA in vivo.

ISBN: 0496782517Subjects--Topical Terms:

1017835
Biology, Animal Physiology.

Estrogen regulation of gene expression and analysis of the role of the coregulator, repressor of estrogen receptor activity (REA).
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020 $a 0496782517
035 $a (UnM)AAI3131003
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040 $a UnM $c UnM
100 1 $a Park, Seongeun. $3 1935470
245 1 0 $a Estrogen regulation of gene expression and analysis of the role of the coregulator, repressor of estrogen receptor activity (REA).
300 $a 148 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-04, Section: B, page: 1621.
500 $a Adviser: Benita S. Katzenellenbogen.
502 $a Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004.
520 $a To understand estrogen regulation of gene expression and the role of the coregulator, repressor of estrogen receptor activity (REA), I analyzed the 5'-regulatory region of the Na+/H + exchanger regulatory factor/ezrin-radixin moesin binding protein 50 (NHE-RF/EBP50) gene and developed REA knockout mice to characterize the role of REA in vivo.
520 $a NHE-RF (EBP50) is a primary response gene under estrogen receptor (ER) control that may provide a link between estrogen action and the regulation of cytoskeletal and cell signaling pathways. The 5'-regulatory region of the human NHE-RF gene contains many (13) half-estrogen response elements (EREs), but no palindromic full EREs. Transfection assays and electrophoretic mobility and competitive gel shift assays demonstrated direct ER interaction with the multiple half-ERE sites and the importance of the one proximal half-ERE and the multiple upstream half-EREs for eliciting the robust transcription activation of the NHE-RF gene by the estrogen-ER complex. These findings highlight a paradigm for gene regulation via numerous half-ERE sites that expands the range of modes by which DNA recognition sites mediate the actions of this nuclear receptor.
520 $a To elucidate the functional activities of REA in diverse estrogen target tissues in vivo, I have used targeted disruption to ablate the REA gene in mice. Genotyping revealed that homozygous animals are not viable, suggesting a crucial role for REA in early development. The viability of heterozygous animals is similar to that of wild type, and female heterozygous animals have an increased body weight relative to age-matched wild-type animals beginning after puberty. Studies in immature heterozygous animals revealed a greater uterine weight gain in response to estradiol (E2) and a greater stimulation of E2 up-regulated genes and a loss of down regulation in genes normally suppressed by E2 in the uterus. Analysis of the histology of the uterus and mammary gland in REA wild type and heterozygous mice revealed gene dosage developmental effects of REA and changes in E2-responsiveness. Studies using mouse embryo fibroblasts (MEFs) revealed that REA heterozygous MEFs displayed a greater transcriptional response to E2. These studies demonstrate that REA is a significant modulator of estrogen responsiveness in vivo.
590 $a School code: 0090.
650 4 $a Biology, Animal Physiology. $3 1017835
650 4 $a Biology, Molecular. $3 1017719
690 $a 0433
690 $a 0307
710 2 0 $a University of Illinois at Urbana-Champaign. $3 626646
773 0 $t Dissertation Abstracts International $g 65-04B.
790 1 0 $a Katzenellenbogen, Benita S., $e advisor
790 $a 0090
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3131003