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Characterization of root stimulating...

Sharma, Anjana Rajen.

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Characterization of root stimulating bacterium and its root stimulating abilities.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterization of root stimulating bacterium and its root stimulating abilities./
Author:	Sharma, Anjana Rajen.
Description:	114 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4713.
Contained By:	Dissertation Abstracts International64-10B.
Subject:	Biology, Botany. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3107681
ISBN:	0496552295

Characterization of root stimulating bacterium and its root stimulating abilities.
Sharma, Anjana Rajen.

Characterization of root stimulating bacterium and its root stimulating abilities. - 114 p.

Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4713.

Thesis (Ph.D.)--The University of Tennessee, 2003.

In this study, the characterization of a novel root stimulating bacterium (RSB) is reported and the root stimulating ability of RSB is compared to Sphingomonas and other related bacteria. A morphological examination of RSB was conducted. Nutritional and biochemical characterization was performed in order to obtain a profile of RSB. Pigment analysis indicated the presence of nostoxanthin, a pigment commonly found in Sphingomonas bacteria. Lipid analysis of RSB revealed the presence of sphingolipids in addition to other fatty acids suggesting similarity with other Sphingomonas bacteria. Pulse-field gel electrophoresis of RSB and Sphingomonas echinoides showed a high level of difference in the DNA of these two organisms suggesting difference at least at the strain level.

ISBN: 0496552295Subjects--Topical Terms:

1017825
Biology, Botany.

Characterization of root stimulating bacterium and its root stimulating abilities.
LDR:02737nmm 2200313 4500 001 1845608
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020 $a 0496552295
035 $a (UnM)AAI3107681
035 $a AAI3107681
040 $a UnM $c UnM
100 1 $a Sharma, Anjana Rajen. $3 1933763
245 1 0 $a Characterization of root stimulating bacterium and its root stimulating abilities.
300 $a 114 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4713.
500 $a Adviser: Otto J. Schwarz.
502 $a Thesis (Ph.D.)--The University of Tennessee, 2003.
520 $a In this study, the characterization of a novel root stimulating bacterium (RSB) is reported and the root stimulating ability of RSB is compared to Sphingomonas and other related bacteria. A morphological examination of RSB was conducted. Nutritional and biochemical characterization was performed in order to obtain a profile of RSB. Pigment analysis indicated the presence of nostoxanthin, a pigment commonly found in Sphingomonas bacteria. Lipid analysis of RSB revealed the presence of sphingolipids in addition to other fatty acids suggesting similarity with other Sphingomonas bacteria. Pulse-field gel electrophoresis of RSB and Sphingomonas echinoides showed a high level of difference in the DNA of these two organisms suggesting difference at least at the strain level.
520 $a The root stimulating ability of RSB, Sphingomonas and other related bacteria were evaluated using mung bean bioassays. Results of these bioassays suggest that the spent medium used for culturing RSB and Sphingopyxis macrogoltabida contain acidic and neutral compounds that contribute to their promotion of adventitious root formation whereas the spent medium used to culture Novosphingobium capsulata, Sphingomonas echinoides and Sphingomonas paucimobilis contains root stimulating components of acidic nature. Thin layer chromatography of acidic fractions obtained from RSB, Sphingomonas echinoides, Sphingomonas paucimobilis, Sphingopyxis macrogoltabida and Novosphingobium capsulata spent growth medium showed Rf values close to those for the standards indolelactic acid (ILA) and indoleacetic acid (IAA).
520 $a Based on all these tests, RSB most closely resembles bacteria of the Sphingomonas group and the rooting ability observed for RSB is characteristic of some of the Sphingomonas and related bacteria.
590 $a School code: 0226.
650 4 $a Biology, Botany. $3 1017825
650 4 $a Biology, Plant Physiology. $3 1017865
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0309
690 $a 0817
690 $a 0410
710 2 0 $a The University of Tennessee. $3 1022026
773 0 $t Dissertation Abstracts International $g 64-10B.
790 1 0 $a Schwarz, Otto J., $e advisor
790 $a 0226
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3107681